Project Number: 3607-22000-011-08
Project Type: Specific Cooperative Agreement

Start Date: Jun 30, 2006
End Date: Apr 14, 2010

Objective:
The objective of this cooperative research project is to develop a transient expression vector based on the negative stranded RNA genomes of the nucleorhabdovirus Maize mosaic virus (MMV).

Approach:
1. Construction a of transient expression vector based on MMV genome in yeast; 2. Analysis of yeast cells and cell lines from diverse organisms for susceptibility to MMV invasion and replication; and, 3. Optimization of the vascular puncture inoculation (VPI) for introduction of wild type and recombinant MMV into maize plants.