Zhu YS, Stricker R, Gong Y, Kiprov D, Isaacs S, Cimino G; International Conference on AIDS.
Int Conf AIDS. 1992 Jul 19-24; 8: B194 (abstract no. PoB 3623).
HRI Research Inc., Concord.
OBJECTIVE: To assess the range and variability of plasma viremia in HIV seropositive individuals, we developed a quantitative RT-PCR assay for HIV and applied this assay to plasma samples of asymptomatic, ARC and AIDS patients. METHODS: The plasma RNA from 38 HIV-1 seropositive patients (5 asymptomatic, 13 ARC and 20 AIDS) and 15 HIV-1 seronegative individuals was extracted with a guanidinium solution. These samples were reverse transcribed, amplified by PCR, and detected by oligomer hybridization to a 32P probe. Quantitation was achieved by the concurred amplification of an HIV cRNA standard. The cRNA standard was synthesized by T7 RNA polymerase using a plasmid pBKBH10S DNA as the template. Each patient's plasma was diluted appropriately to be within the quantitative range of the assay. RESULTS: The HIV-1 RNA assay was quantitative over a linear range from 50 to 1000 input copies. All 38 HIV-1 seropositive patients were tested as positive by RT-PCR. The HIV-1 RNA copies in plasma varied widely among the patients from 800 to 500,000 copies/ml plasma. We have found that the increase in HIV-1 RNA in plasma is related to a progressive decline in CD4+ lymphocytes and deteriorating clinical course in HIV-1-infected patients. Among 15 HIV-1 seronegative individuals one high risk person was treated as positive by RT-PCR. CONCLUSION: Quantitation of HIV-1 RNA by RT-PCR may provide useful tool for understanding HIV pathogenesis and monitoring antiviral drug therapy.
Publication Types:
Keywords:
- AIDS-Related Complex
- Acquired Immunodeficiency Syndrome
- CD4-Positive T-Lymphocytes
- HIV Infections
- HIV Seropositivity
- HIV-1
- Humans
- Nucleic Acid Hybridization
- Plasma
- Polymerase Chain Reaction
- RNA
- RNA, Viral
- Reverse Transcriptase Polymerase Chain Reaction
- Viremia
- reverse transcriptase, Human immunodeficiency virus 1
Other ID:
UI: 102199063
From Meeting Abstracts