Hulme P, Raviglione M, Currier J, Hanff P; American Society for Microbiology. Meeting.
Abstr Annu Meet Am Soc Microbiol. 1991 May 5-9; 91: 349 (abstract no. C-41).
Beth Israel Hospital, Harvard Medical School, Boston, MA.
Recent data suggest that the detection of Pneumocystis carinil (Pc) is more difficult in patients on aerosolized pentamidine (AP) prophyiaxis. To examine this issue, we quantitated the number of Pc cysts, trrophozoites, and matrix on positive slides made May 1989-November 1990 using a direct immunofluorescent monoclonal antibody (DFA) test. 2ml of processed induced sputum were used to prepare slides by the cytospin technique. Slides were stained by DFA and the entire smear was examined. The number of individual cysts and trophozoites and the number of clusters and organisms within each cluster was noted. Twenty-five specimens were from patients on AP and 36 were from patients not on prophylaxis. The results are as follows. TABULAR DATA, SEE ABSTRACT VOLUME. Further, 28% of patients on AP had less than 10 matrix present in contrast to 8.3% for those not on AP. In conclusion, by using the DFA, prior prophylaxis with AP did not affect the ability to detect Pc in poistive induced sputa. Moreover, patients on AP tended to have a higher burden of cysts, trophozoites, and matrix when compared to those who had not received prophylaxis.
Publication Types:
Keywords:
- Alkaline Phosphatase
- Animals
- Antibodies, Monoclonal
- Coloring Agents
- Cysts
- Humans
- Pentamidine
- Pneumocystis
- Pneumocystis Infections
- Pneumonia, Pneumocystis
- Protozoa
- Specimen Handling
- Sputum
- Staining and Labeling
- parasitology
Other ID:
UI: 102181554
From Meeting Abstracts