Viswanath R, Jacob M, Samuel NM, Ahamed S, Joshi PL; International Conference on AIDS.

Int Conf AIDS. 2002 Jul 7-12; 14: abstract no. WePeB5939.

Tn.dr.mgr.medical university, Chennai, India

BACKGROUND: Qualitative DNA PCR is used to monitor HIV-1 status in infants. Earlier detection of HIV-1 in infants will help in deciding breast feeding a child. In India risk of vertical transmission of HIV-1 is 48%(NACO).To reduce this risk of transmission National AIDS Control Organisation has conducted Fesibility study of Administering short term AZT intervention amoung HIV infected mothers to prevent mother to child transmission of HIV at 11 centers. Samples for DNA PCR is sent to our laboratory from 5 centers. OBJECTIVE: Importance of Qualitative DNA PCR for infant diagnosis. METHOD: Whole blood Samples for DNA PCR are sent from 5 medical institutions in south India to our PCR referral laboratory. Samples are collected and sent to us at two intervals one at 48hrs after birth and another at 2 months of age.DNA is extracted from whole blood using standard procedure. Nested PCR is performed on these samples using HIV-1 gag primers. RESULTS: The results of 5 MTCT centers are out of 225 babies delivered 30 were found to be positive at 48 hrs. of this 30 positive babies, only 10 turned up for follow up at 2nd month and diagnosed positive. All the 10 positives were positive at birth. Positive 30(13.3%) - Day Zero 12(13.4%) - 2nd Month Negative 195(86.7%)- Day Zero 78(86.6%) - 2nd Month Total 225 90 Out of 195 negatives in day zero only 90 turned up for follow up. Among the 90 negatives 2* were turned positive in 2nd month. Of the total 225 babies 60% of babies did not turned up for follow up. CONCLUSION: *Qualitative DNA PCR is essential for diagnosis of HIV-1 in infants. *As this technique require expensive equipments central facility can be established where others can utilize it. * adherence to the procedure of the technique.

Publication Types:

• Meeting Abstracts

Keywords:

• Acquired Immunodeficiency Syndrome
• Breast Feeding
• Child
• DNA
• DNA, Viral
• Disease Transmission, Vertical
• HIV
• HIV Antibodies
• HIV Core Protein p24
• HIV Infections
• HIV Seropositivity
• HIV-1
• Humans
• India
• Infant
• Mothers
• Polymerase Chain Reaction
• diagnosis
• immunology
• transmission

Other ID:

• GWAIDS0017494

UI: 102254992

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