Ohlsson-Wilhelm BM, Eyster ME, Frieberg G, Cimoch PJ, Jaworski M, Kulgavin A, Seyedzadeh M, Jackowski J, Reiter WR; International Conference on AIDS.
Int Conf AIDS. 1991 Jun 16-21; 7: 74 (abstract no. TH.A.71).
The Pennsylvania State University, M.S. Hershey Med Ctr., Hershey, PA
OBJECTIVE: To quantify HIV-infected lymphocytes in individuals at different stages of infection. METHODS: Individuals with HIV disease (N=33) receiving antiretroviral therapy were grouped according to CD4 count: Group I (N=18), CD4 counts between 200 and 500/mm(3); and Group II (N=15), CD4 less than 200/mm(3). Addition of a phycoerythrin-labelled, CD4-specific, monoclonal antibody to the standard lymphocyte p24-FCA assay (J. Inf. Dis. 162:1018-1024, 1990), a flow cytometric measure of HIV infection, allowed quantification of lymphocytes with cell-associated HIV-p24 antigen (P24+ lymphocytes) in both the CD4+ and CD4- lymphocyte compartments. TABULAR DATA, SEE ABSTRACT VOLUME. DISCUSSION AND CONCLUSION: Lymphocyte p24-FCA can be used to monitor HIV infection of lymphocyte subsets at different stages of infection. As CD4 counts fall, the percentage of p24+ cells rises, indicating an increasing viral load.
Publication Types:
Keywords:
- Acquired Immunodeficiency Syndrome
- Antigens, CD4
- CD4 Lymphocyte Count
- CD4-Positive T-Lymphocytes
- HIV Core Protein p24
- HIV Infections
- HIV Seropositivity
- Immunologic Techniques
- Lymphocyte Count
- Lymphocytes
- Viral Load
- immunology
- organization & administration
- physiology
Other ID:
UI: 102196815
From Meeting Abstracts