Saag M, Decker D, Campbell S, Crain M, Leuther M, Shaw G; International Conference on AIDS.
Int Conf AIDS. 1990 Jun 20-23; 6: 162 (abstract no. F.A.346).
Univ. of Alabama at Birmingham, Birmingham, AL, USA
OBJECTIVE: To determine the level of plasma viremia in HIV infected children and adults and correlate the presence of viremia to clinical disease status. METHODS: Sixty-six adult and 11 children infected with HIV were identified and clinically evaluated. Peripheral blood was collected in ACD tubes, centrifuged, and the plasma component passed through a 1.2mu filter, serially diluted, cocultivated with 10(6) PHA stimulated donor lymphocytes for 28 days, and sampled twice weekly for p24 Ag. RESULTS: Plasma viremia was detected in 19/64 adults and 5/11 children with a mean titer of 10(-2.8) (range 10(0)-10(-6). Among the adults, 0/17 asymptomatic, 4/33 ARC, and 15/16 AIDS patients were viremic. When examined versus (CD4 count), 0/16 (greater than 600), 1/21 (400-600), 4/13 (200-400), and 14/16 (less than 200) patients were viremic. Among the children, 0/4 P-O versus 5/7 symptomatic children were viremic. No association between CD4 counts and viremia was noted in children. Evaluation of p24 Ag versus viremia revealed: TABULAR DATA, SEE ABSTRACT VOLUME. Among viremic patients subsequently treated with AZT, 8/8 demonstrated a decrease in virus titer of 10(-1) to 10(-4) from pretreatment titers. CONCLUSIONS: (1) Free HIV may exist in plasma and can be quantified; (2) a striking correlation exists between stage of disease, CD4 count, and viremia in adults; (3) children with symptomatic disease are likely to be viremic regardless of CD4 count; (4) a positive correlation exists between viremia and p24 antigenemia; (5) plasma viremia may be especially useful in the evaluation of antiretroviral compounds.
Publication Types:
Keywords:
- AIDS Vaccines
- AIDS-Related Complex
- Acquired Immunodeficiency Syndrome
- Adult
- Anti-HIV Agents
- CD4 Lymphocyte Count
- Child
- HIV
- HIV Antibodies
- HIV Antigens
- HIV Core Protein p24
- HIV Infections
- HIV Seropositivity
- Humans
- Plasma
- Viremia
- Zidovudine
- immunology
Other ID:
UI: 102184252
From Meeting Abstracts