Gurtler L, Zekeng L, Eberle J, von Brunn A, Kaptue L; International Conference on AIDS.
Int Conf AIDS. 1996 Jul 7-12; 11: 276 (abstract no. Tu.A.2062).
University of Munchen, Munchen, Germany. Fax: 49-89-538 0584.
Objective: To analyze the mutation rate of HIV-O strains in patients during follow up Material and Methods: From two Cameroonian patients in two bleedings after 3 and 18 months, respectively, the HIV could be isolated by PHA stimulation of PBLs. The virus was propagated on PBL and HUT-78 cells, DNA extracted and the V3 loop sequenced by PCR amplified sequencing using the conserved primers 5 cam-env and 3 cam-env. From the nucleic acid sequence the amino acid sequence was delined and aligned to known HIV-O sequences. Results: From the first patient A 3 isolates were obtained that showed in the V3 loop within the cysteine residues 5 (14% of 37) exchanges between isolate 1 and 2 and additional 3 (8% of 37) +2 exchanges between isolates 2 and 3. In isolate 3 one exchange was a remutation of Arg to Lys, the other of Glu to Lys. In patient B after 18 months 6 exchanges were found corresponding to 15% of the 39 amino acids building the loop. Conclusion: Heterogeneity of HIV-O V3 loop sequences in follow up isolates is high and indicates either a high error rate of the reverse transcriptase of HIV-O comparable to HIV-1 and/or a considerable induction of immune selection. Both patients are alive since follow up for now more than 2 years and both have clinical symptoms ranking them to C2 of the CDC/WHO scheme. (table: see text)
Publication Types:
Keywords:
- Acquired Immunodeficiency Syndrome
- Amino Acid Sequence
- Base Sequence
- Genes, env
- HIV Infections
- HIV Seropositivity
- HIV-1
- Humans
- Polymerase Chain Reaction
- genetics
Other ID:
UI: 102218176
From Meeting Abstracts