Project Number: 5348-21000-023-01
Project Type: Reimbursable

Start Date: Oct 01, 2004
End Date: Sep 30, 2009

Objective:
1) Evaluate cold tolerance of winter wheat recombinant inbred and near isogenic wheat populations by quantifying cold tolerance per se, and vernalization response. 2) Identify QTL's associated with those traits and develop molecular markers for use in marker-assisted selection for cold tolerance. 3) Fine map the frost resistance gene (Fr2) on chromosome 5A in diploid Triticum monococcum. 4) Characterize the CBF gene family indiploid wheat, determine which CBF genes are linked to Fr2, and compare locations of CBF genes with locations of QTL's for cold tolerance identified above.

Approach:
Methods used include artificial freeze tests, determination of flowering response under increasing daylight regines, linkage analysis using microsatellites, AFLPs, RFLPs, SNPs and candidate gene sequeces, analysis of diploid wheat BAC libraries. Statistical methods used include analysis of variance and maximum likelihood estimation of linkage. Results will provide genetic resources for mapping and cloning cold tolerance genes in wheat and more efficient methods of selection for cold tolerance. Results will also be applicable to other crops (for example winter barley). Documents Reimbursable with NRI. Log 25968. Formerly 5348-21220-003-06R (5/2008).