2005 Annual Report 4d.Progress report. This report serves to document research conducted under a National Pork Board Grant to USDA ARS BARC APDL and University of Iowa scientists. Additional details of research can be found in the report from the parent project 1265-32000-064-00D: Strategies to Control Swine Parasites Affecting Food Safety. Understanding disease and vaccine responses requires improved antibody reagents for pigs. Current pig antibody reagents can distinguish the broad classes of immunoglobulins (Igs), the IgGs from IgM and IgA; however, there are no IgG subclass reagents, nor reagents for IgE, the Ig associated with allergies and asthma. This National Pork Board Grant (1265-32000-064-10R) to USDA ARS BARC APDL and University of Iowa scientists will provide funding to develop, for all swine researchers, monoclonal antibodies (mAb) reactive with the different IgG subclass proteins and with IgE. These reagents will give researchers better ways to define, and ultimately target, the level of specific antibody (Ig) responses to infectious disease and for vaccine studies. The anti-Ig mAb will help determine which swine Ig subclass genes and proteins are most important for specific immune functions, e.g., clearing bacterial and parasitic infections or neutralizing virus load. 7.List your most important publications in the popular press and presentations to organizations and articles written about your work. (NOTE: List your peer reviewed publications below). J.K. Lunney. Immune Tools Measure Pig Disease, Vaccine Responses. National Hog Farmer Magazine (Popular Press); December, 15, 2004. p.13-14.