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Allyl Propyl Disulfide
Diallyl Disulfide
Dipropyl Disulfide
Related Information: Chemical Sampling -
Allyl Propyl Disulfide,
Diallyl Disulfide,
Dipropyl Disulfide
|
Method no.: |
PV2086 |
|
Control no.: |
T-PV2086-01-F8305-CH |
|
Matrix: |
Air |
|
Target concentration: |
2 ppm (12 mg/m3) allyl propyl disulfide OSHA TWA PEL |
|
Procedure: |
Samples are collected by drawing a known volume of air through aChromosorb 106 tube.
Samples are desorbed with trichloroethylene and analyzed by gas chromatography using a flame photometric detector (GC-FPD).
|
|
Air volume and sampling rate studied: |
10 liters at 0.2 Lpm |
|
Status of method: |
Partially Validated Method. This method has been only partially evaluated andis presented for information and trial use. |
|
May 1983 |
Mary E. Eide |
|
|
Methods Development Team
Industrial Hygiene Chemistry Division
OSHA Salt Lake Technical Center
Sandy UT 84070-6406 |
1. General Discussion
1.1. Background
1.1.1 History of procedure
The OSHA laboratory recently received some samples collected in toluene impingers requesting allyl propyl disulfide.
A solid sorbent collection method was wanted, so XAD-4, Tenax,and Chromosorb 106 tubes were investigated, and
Chromosorb 106 tubes were found to have the best desorption efficiency. The retention and storage studies with Chromosorb
106 were good.
1.1.2 Potential workplace exposure (Ref. 5.1)
Workers are exposed to allyl propyl disulfide, diallyl disulfide, and dipropyl disulfide in onion and garlic processing plants.
1.1.3 Toxic effects (This section is for information purposes and should not be taken as the basis for OSHA
policy.) (Refs. 5.1-5.7)
The OSHA PEL of 2 ppm for allyl propyl disulfide is based on study of worker exposure in an onion processing plant in 1946 by Feiner et al (Ref.
5.1). They took air samples in gas bags, oxidized the contents, and analyzed for total sulfur dioxide. They assumed the atmosphere sampled was all allyl propyl disulfide and calculated the amount allyl propyl disulfide present based on the amount of sulfur dioxide found. These amounts averaged 3.4 ppm. Since the workers at the plant had eye and skin
problems from exposure, they recommended a PEL of 2 ppm. Grant recommends a PEL of 2-3 ppm for diallyl disulfide based on its presence in cut onion vapor (Ref.
5.2). Onions, when cut, form allyl propyl disulfide, diallyl disulfide, dipropyl disulfide, other disulfides, sulfides, trisulfides, thiosulfinates, sulfenic acids, mercaptans, sulfoxides, sulfates, and thial
oxides (Ref. 5.3). All of these compounds form sulfur dioxide when oxidized and the assumption by Feiner et al that the compound measured was all allyl propyl disulfide, or in the cased of Grant allyl propyl disulfide and diallyl disulfide, may be erroneous. The concentration
of allyl propyl disulfide, diallyl disulfide, and dipropyl disulfide changes with time after the onion is cut, with more found with time (Ref.
5.4). These concentrations are also dependant upon the variety of onion sampled. Some researchers found no allyl propyl disulfide in the vapor from some of the varieties of onion studied (Ref.
5.5). Block et al have suggested that the lachrimatory factor in onions is propanethial-S-oxide, which forms sulfuric acid immediately upon contact with water (Ref.
5.6). Burning of the throat and eyes was observed at
the laboratory when trace levels of allyl propyl disulfide, diallyl disulfide, and dipropyl disulfide were released into the air by washing volumetrics in a dishwasher. The volumetrics had been allowed to dry before washing for five days (Ref.
5.7). This data suggest toxicity studies should be performed using the individual
compounds mentioned and the PEL re-evaluated based on the new data.
1.1.4 Physical properties:
|
|
Allyl propyl
disulfide (Ref. 5.8)
|
Compound: |
H2C=CHCH2S2CH2CH2CH3 |
Molecular formula: |
C6H12S2 |
Synonyms: |
Disulfide, allyl propyl |
Molecular weight: |
148.16 |
Density: |
0.9289 |
Freezing point: |
-15ºC |
Odor: |
onion odor |
Color: |
very pale yellow oil |
CAS: |
2179-59-1 |
IMIS: |
0150 |
RTECS: |
J00350000; 32322 |
|
|
Diallyl disulfide
(Ref. 5.9)
|
Compound: |
H2C=CHCH2S2CH2CH=CH2 |
Molecular formula: |
C6H10S2 |
Synonyms: |
Allyl disulfide; Di-2-propenyldisulfide;
4,5-Dithia-1,7-octadiene |
Molecular weight: |
146.26 |
Density: |
1.01 |
Boiling point: |
79ºC |
Odor: |
garlic odor |
Color: |
pale yellow oil |
CAS: |
2179-57-9 |
IMIS: |
D736 |
Dipropyl disulfide (Ref. 5.10)
|
Compound: |
H3CCH2CH2S2CH2CH2CH3 |
Molecular formula: |
C6H14S2 |
Synonyms: |
Di-n-propyl disulfide; Propyl
disulfide |
Molecular weight: |
150.31 |
Density: |
0.9599 |
Boiling point: |
193.5ºC |
Odor: |
onion odor |
Color: |
pale yellow oil |
CAS: |
629-19-6 |
IMIS: |
D626 |
1.2 Limit defining parameters
1.2.1 The detection limit of the analytical procedure is 1 µg for each of allyl propyl disulfide, diallyl disulfide, and dipropyl disulfide. This is the smallest amount that could be
detected under normal operating conditions.
1.2.2 The overall detection limit is 0.02 ppm for each of allyl propyl disulfide, diallyl disulfide, and dipropyl disulfide. (All ppm amounts in this study are based on a 10 liter air volume.)
1.3 Advantages
1.3.1 The sampling procedure is convenient.
1.3.2 The analytical method is reproducible and
sensitive.
1.3.3 Reanalysis of samples is possible.
1.3.4 It may be possible to analyze other compounds at the same time.
1.3.5 Interferences may be avoided by proper selection of column and GC parameters.
1.4 Disadvantages
none known
2. Sampling procedure
2.1 Apparatus
2.1.1 A calibrated personal sampling pump, the flow of which can be determined within ±5% at the recommended flow.
2.1.2 Chromosorb 106 tubes containing 100 mg adsorbing section with 50 mg backup section, separated by urethane foam plug with silanized glass wool before the adsorbing section and urethane foam at the back of the backup section. The ends are flame sealed and the glass tube containing the adsorbent is 7 cm long with 6 mm O.D. and 4 mm I.D., SKC tubes or equivalent.
2.2 Sampling technique
2.2.1 Open the ends of the Chromosorb 106 tube immediately before sampling.
2.2.2 Connect the Chromosorb 106 tube to the sampling pump with flexible tubing.
2.2.3 Place the tubes in a vertical position to
minimize channeling, with the smaller section towards the pump.
2.2.4 Air being sampled should not pass through anyhose or tubing before entering the Chromosorb 106 tube.
2.2.5 Seal the Chromosorb 106 tube with plastic caps immediately after sampling. Seal each sample lengthwise with OSHA
Form 21 sealing tape.
2.2.6 With each batch of samples, submit at least one blank tube from the same lot used for samples. this tube should be subjected to exactly the same handling as the samples (break ends, seal, & transport) except no air is drawn through it.
2.2.7 Transport the samples (and corresponding paperwork) to the lab for analysis.
2.2.8 Bulks submitted for analysis must be shipped in a separate mailing container from other samples.
2.3 Desorption efficiency
2.3.1 Allyl propyl disulfide
Six tubes were spiked at each loading of 64.25 µg (1.06 ppm), 120.8 µg (1.99 ppm), and 242.4
µg (4.00 ppm) allyl propyl disulfide. They were allowed to equilibrate overnight at room temperature. They were opened, each section placed into a separate 2
mL vial, desorbed with 1 mL of trichloroethylene for 30 minutes with occasional shaking, and were analyzed by GC-FPD. The overall average was
96.83%. (Table 1)
Table 1
Allyl Propyl Disulfide Desorption Efficiency
|
|
|
% Recovered |
Tube# |
64.25 µg |
120.8 µg |
242.4 µg |
|
1 |
96.95 |
90.86 |
90.10 |
2 |
94.01 |
96.47 |
94.41 |
3 |
96.25 |
92.16 |
98.85 |
4 |
95.13 |
96.36 |
99.56 |
5 |
97.64 |
99.29 |
101.0 |
6 |
98.42 |
101.5 |
104 |
average |
96.40 |
96.11 |
97.98 |
overall average |
96.83 |
|
standard deviation |
± 3.69 |
|
|
2.3.2 Diallyl disulfide
Six tubes were spiked at each loading of 65.65 µg (1.10 ppm), 131.3 µg (2.19 ppm), and 262.6
µg
(4.39 ppm) diallyl disulfide. They were allowed to equilibrate overnight at room temperature. they were opened, each section placed into a separate 2
mL vial, desorbed with 1
mL of trichloroethylene for 30 minutes with occasional shaking, and were analyzed by GC-FPD.(Table 2)
Table 2
Diallyl Disulfide Desorption Efficiency
|
|
|
% Recovered |
Tube# |
65.65 µg |
131.3 µg |
262.6 µg |
|
1 |
89.50 |
101.4 |
100.8 |
2 |
86.44 |
101.2 |
91.52 |
3 |
84.79 |
96.48 |
99.49 |
4 |
88.09 |
103.5 |
98.50 |
5 |
84.56 |
102.6 |
102.2 |
6 |
85.54 |
102.8 |
107.0 |
average |
86.49 |
101.3 |
99.93 |
|
2.3.3 Dipropyl disulfide
Six tubes were spiked at each loading of 62.39 µg (1.01 ppm), 124.8 µg (2.03 ppm), and 249.6
µg (4.06 ppm) dipropyl disulfide. They were allowed to equilibrate overnight at room temperature. they were opened, each section placed into a separate 2
mL vial, desorbed with 1
mL of trichloroethylene for 30 minutes with occasional shaking, and were analyzed by GC-FPD.
(Table 3)
Table 3
Dipropyl Disulfide Desorption Efficiency
|
|
|
% Recovered |
Tube# |
62.39 µg |
124.8 µg |
249.6 µg |
|
1 |
85.24 |
95.72 |
97.18 |
2 |
79.73 |
97.54 |
101.3 |
3 |
80.72 |
97.30 |
102.1 |
4 |
81.81 |
94.66 |
101.1 |
5 |
85.45 |
95.72 |
101.9 |
6 |
80.52 |
92.97 |
104.87 |
average |
82.25 |
95.65 |
101.4 |
|
2.4 Retention Efficiency
2.4.1 Allyl propyl disulfide
Since pure allyl propyl disulfide was expensive and difficult to obtain, the lab purchased only a small quantity. This was used up in the desorption studies. A mixture of allyl propyl disulfide, diallyl disulfide and dipropyl disulfide in a ratio of 42.75:10.91:46.34 respectively, was used for the retention and storage studies. Six tubes were liquid spiked with 124.8
µg (2.06 ppm) allyl propyl disulfide, allowed to equilibrate overnight, and had 10 liters humid air (80% RH) pulled through them at 0.1 Lpm. They were opened, desorbed and analyzed by GC-FPD. The retention efficiency averaged 98.95%. There was no allyl propyl
disulfide found on the backup portions of the tubes. (Table 4)
Table 4
Allyl Propyl Disulfide Retention Efficiency
|
Tube # |
% Recovered
'A' |
% Recovered 'B' |
Total |
|
1 |
99.17 |
0.0 |
99.17 |
2 |
97.32 |
0.0 |
97.32 |
3 |
99.80 |
0.0 |
99.80 |
4 |
104.91 |
0.0 |
104.91 |
5 |
95.78 |
0.0 |
95.78 |
6 |
96.70 |
0.0 |
96.70 |
|
|
|
|
|
|
average |
98.95 |
|
2.4.2 Diallyl disulfide
Six tubes were liquid spiked with 131.3 µg (2.19 ppm) diallyl disulfide, allowed to equilibrate overnight, and had 10 liters humid air (80% RH) pulled through them at 0.1 Lpm. They were
opened, desorbed and analyzed by GC-FPD, The retention efficiency averaged 98-32%. There was no diallyl disulfide found on the backup portions of the
tubes. (Table 5)
Table 5
Diallyl Disulfide Retention Efficiency
|
Tube # |
% Recovered 'A' |
% Recovered 'B' |
Total |
|
1 |
94.59 |
0.0 |
94.59 |
2 |
100.37 |
0.0 |
100.37 |
3 |
101.56 |
0.0 |
101.56 |
4 |
97.29 |
0.0 |
97.29 |
5 |
95.56 |
0.0 |
95.56 |
6 |
100.52 |
0.0 |
100.52 |
|
|
|
|
|
|
average |
98.32 |
|
2.4.3. Dipropyl disulfide
Six tubes were liquid spiked with 124.8 µg (2.03 ppm) dipropyl disulfide, allowed to equilibrate overnight, and had 10 liters humid air (80% RH) pulled through them at 0.1 Lpm. They were opened, desorbed and analyzed by GC-FPD. The retention efficiency averaged 99.43%. There was no dipropyl disulfide found on the backup portions of the
tubes. (Table 6)
Table 6
Dipropyl Disulfide Retention Efficiency
|
Tube # |
% Recovered 'A' |
% Recovered 'B' |
Total |
|
1 |
98.32 |
0.0 |
98.32 |
2 |
98.01 |
0.0 |
98.01 |
3 |
101.81 |
0.0 |
101.81 |
4 |
105.68 |
0.0 |
105.68 |
5 |
95.59 |
0.0 |
95.59 |
6 |
97.15 |
0.0 |
97.15 |
|
|
|
|
|
|
average |
99.43 |
|
2.5 Storage
2.5.1 Allyl propyl disulfide
Tubes were spiked with 124.8 µg (2.06 ppm) allyl propyl disulfide, and stored at refrigerated
(0ºC) and room
(24ºC) temperatures until opened and analyzed. The refrigerated temperature recoveries averaged 98.21% and the room temperature recoveries averaged 99.70% for allyl propyl disulfide for the 6 days
stored. (Table 7)
Table 7
Allyl Propyl Disulfide Storage Study
|
|
% Recovered |
Day |
0ºC |
24ºC |
|
4 |
97.20 |
101.8 |
4 |
103.2 |
98.64 |
4 |
98.93 |
95.67 |
6 |
99.57 |
97.64 |
6 |
98.44 |
97.45 |
6 |
100.8 |
98.03 |
|
|
|
average |
99.70 |
98.21 |
|
2.5.2 Diallyl disulfide
Tubes were spiked with 131.3 µg (2.19 ppm) diallyl disulfide, and stored at refrigerated
(0ºC) and room
(24ºC) temperatures until opened and analyzed. The refrigerated temperature recoveries averaged 98.68% and the room temperature recoveries averaged 98.29% for diallyl disulfide for the 12 days
stored. (Table 8)
Table 8
Diallyl Disulfide Storage Study
|
|
% Recovered |
Day |
0ºC |
24ºC |
|
7 |
93.83 |
97.19 |
7 |
90.18 |
93.79 |
7 |
98.98 |
96.09 |
12 |
102.86 |
98.10 |
12 |
103.69 |
103.54 |
12 |
102.54 |
101.00 |
|
|
|
average |
98.68 |
98.68 |
|
2.5.3. Dipropyl disulfide
Tubes were spiked with 124.8 µg (2.03 ppm) dipropyl disulfide, and stored at refrigerated
(0ºC) and room (24ºC) temperatures until opened and analyzed. The refrigerated temperature recoveries averaged 99.01% and the room temperature recoveries averaged 97.28% for dipropyl disulfide for the 9 days
stored. (Table 9)
Table 9
Dipropyl Disulfide Storage Study
|
|
% Recovered |
Day |
0ºC |
24ºC |
|
3 |
99.21 |
99.75 |
3 |
96.49 |
96.71 |
3 |
100.58 |
99.88 |
9 |
97.41 |
96.91 |
9 |
99.42 |
94.21 |
9 |
100.92 |
96.20 |
|
|
|
average |
99.01 |
97.28 |
|
2.6. Air volume and sampling rate studied
2.6.1 The air volume studied was 10 liters.
2.6.2 The sampling rate studied was 0.2 liters per minute.
2.7 Interferences
Suspected interferences should be listed on sample data sheets.
2.8 Safety precautions
2.8.1 Sampling equipment should be placed on an
employee in a manner that does not interfere with work performance or safety.
2.8.2 Safety glasses should be worn at all times in designated areas.
2.8.3 Follow all safety practices that apply to the workplace being sampled.
3. Analytical method
3.1 Apparatus
3.1.1 Gas chromatograph equipped with a flame
photometric detector with a sulfur filter.
3.1.2 GC column capable of separating the analyte from any interferences. The column used in this study was a 10 ft.
× 1/8 inch stainless steel column packed with 20% SP2100 with 0.1% Carbowax 1500 on 80/100 Supelcoport. An alternate column is a 60 meter
DB-1 capillary column with a 1.0 µm film thickness and 0.32 mm I.D.
3.1.3 An electronic integrator or some other suitable method of measuring peak areas.
3.1.4 Two milliliter vials with Teflon-lined caps.
3.1.5 A 10 µL syringe or other convenient size for sample injection.
3.1.6 Pipets for dispensing the desorbing solution. the Glenco 1 mL dispenser was used in this method.
3.1.7 Volumetric flasks - 5 mL and other convenient sizes for preparing standards.
3.2 Reagents
3.2.1 Purified GC grade nitrogen, hydrogen, and air.
3.2.2 Allyl propyl disulfide
3.2.3 Diallyl disulfide, reagent grade
3.2.4 Dipropyl disulfide, reagent grade
3.2.5 Mixture of allyl propyl disulfide, diallyldisulfide, and dipropyl disulfide
3.2.6 Trichloroethylene, reagent grade
3.3 Sample preparation
3.3.1 Sample tubes are opened and the front and backsection of each tube are placed in separate 2 mL vials.
3.3.2 Each section is desorbed with 1 mL of trichloroethylene.
3.3.3 The vials are sealed immediately and allowed to desorb for 30 minutes with occasional shaking.
3.4 Standard preparation
3.4.1 Standards are prepared by diluting a known quantity of allyl propyl disulfide, diallyl disulfide, and dipropyl disulfide with trichloroethylene.
3.4.2 At least two separate stock standards standards should be made.
3.4.3 Dilutions of the stock standards are prepared to bracket the samples. For this study, the standards ranged from 1 to 300
µg/mL of each compound in the trichloroethylene.
3.5 Analysis
3.5.1 Gas chromatograph conditions for 10 ft. × 1/8 inch stainless steel column packed with 20% SP2100 with 0.1% Carbowax 1500 on 80/100 Supelcoport.
Flow rates (mL/min) |
Temperature (ºC) |
|
|
|
|
Nitrogen: |
24 |
Injector: |
160 |
Hydrogen: |
100 |
Detector: |
200 |
Air: |
60 |
Column: |
130 |
Oxygen: |
30 |
|
|
|
|
|
|
Injection size: |
1 µL |
|
|
Chromatogram: |
(see Figure 1) |
|
|
3.5.2 Gas chromatograph conditions for 60 meter DB-1 capillary column with a
1.0µ film thickness and 0.32 mm I.D.
Flow rates (mL/min) |
Temperature (ºC) |
|
|
|
|
Nitrogen(makeup) : |
30 |
Injector: |
240 |
Hydrogen(carrier) : |
2 |
Detector: |
240 |
Air : |
100 |
Column: |
140 |
Hydrogen(detector): |
75 |
|
|
|
|
|
|
Injection size : |
1 µL |
|
|
Chromatogram: |
(see Figure 2) |
|
3.5.3 Peak areas are measured by an integrator or other suitable means.
3.6 Interferences (analytical)
3.6.1 Any compound having the general retention time of the analyte is an interference. Possible interferences should be listed on the sample data sheet. GC parameters should be adjusted if necessary so these interferences will pose no problems.
3.6.2 Retention time data on a single column is not considered proof of chemical identity. Samplesover the target concentration should be confirmed by GC/Mass Spec or other suitable means.
3.7 Calculations
3.7.1 A curve with area counts versus concentration is calculated from the calibration standards.
3.7.2 The area counts for the samples are plotted withthe calibration curve to obtain the concentration of allyl propyl disulfide, diallyl disulfide, and dipropyl disulfide in solution.
3.7.3 To calculate the concentration of analyte in the air sample the following formulas are used:
(µg/mL) (desorption volume)
|
= |
mass of analyte
in sample
|
(desorption efficiency) |
|
|
|
(mass of analyte in sample)
|
= |
number of moles of analyte
|
molecular weight
|
|
|
|
(number of moles)
of analyte
|
(molar volume)
at 25ºC & 760mm
|
= |
volume the analyte will occupy at 25ºC and 760mm |
|
|
(volume analyte occupies) (106)
*
|
= ppm |
(air volume)
|
*all units must cancel.
3.7.4 The above equations can be consolidated to form the following formula. To calculate the ppm of analyte in the sample based on a 10 liter air sample:
(µg/mL)(DV)(24.46)(106)
|
x |
(g)
|
x |
(mg)
|
= ppm |
(10 L) (DE) (MW) |
(1000mg) |
(1000µg) |
µg/mL |
= |
concentration of analyte in sample or standard |
24.46 |
= |
molar volume (liters/mole) at 25ºC and 760 mmHg. |
MW |
= |
molecular weight (g/mole) |
DV |
= |
desorption volume |
10 L |
= |
10 liter air sample |
DE |
= |
desorption efficiency |
3.7.5 This calculation is done for each section of the sampling tube and the results added together.
3.8 Safety precautions
3.8.1 All handling of solvents should be done in a hood.
3.8.2 Avoid skin contact with all chemicals.
3.8.3 Wear safety glasses, gloves and a lab coat at alltimes.
4. Recommendations for further study
The toxic effects of allyl propyl disulfide, diallyl disulfide,and dipropyl disulfide need to be further evaluated. The low levels which cause eye irritation at the OSHA laboratory suggest the PEL may need to be re-evaluated and toxicity
studies be performed at lower levels. These studies should include diallyl disulfide and dipropyl disulfide besides the allyl propyl disulfide. Bolelens et al found allyl propyl disulfide appeared in the vapor only after 120 minutes had elapsed from the time the onions were cut (Ref.
5.4). This suggests the need to further explore the compounds in cut onion vapor. Block et al suggest propanethial-S-oxide as a lacrimatory agent formed in cut onion vapor (Ref. 5.6). This compound is highly unstable and reactive, forming sulfuric acid immediately upon contact with water. This compound should be studied for toxic effects and its relationship with the toxic effects of onion vapor.
Figure 1. An analytical standard of 64.25 µg/mL allyl propyl disulfide, 65.65
µg/mL diallyl disulfide, and 62.39 µg/mL dipropyl disulfide in trichloroethylene, analyzed on a 10 ft.
× 1/8 inch stainless steel column packed with 20% SP2100 with 0.1% Carbowax 1500 on 80/100 Supelcoport. The retention times of the peaks are: trichloroethylene 4.71 min, diallyl disulfide 13.49 min, allyl propyl disulfide 14.55 min, and dipropyl disulfide 15.70 min.
|
Figure 2. An analytical standard of 64-25 µg/mL allyl propyl disulfide-, 65.65
µg/mL diallyl disulfide, and 62.39 µg/mL dipropyl disulfide in trichloroethylene, analyzed on a 60 meter
DB-1 capillary column with a 1.0 µm film thickness and 0.32 mm I.D. The retention times of the peaks are: trichloroethylene 3.37 min, diallyl disulfide 8.06 min, allyl propyl disulfide 8.43 min, and dipropyl disulfide 8.86 min.
|
5. References
5.1 Feiner, B., Burk, W.J., and Baliff, J., J. Ind. Hyg. Toxicol., 1946, p. 278.
5.2 Grant, W.M., "Toxicology of the Eye", 2nd Edition, Charles C. Thomas, Springfield, Illinois, 1974, p. 26.
5.3 Mazza, G., Lemaguer, M., and Hodziyer, D., Can. Inst. Food Sci. Technol. J., 1980, p. 87-96.
5.4.od Chem., 1971, p. 984-991.
5.5 "Advances in Food Research" Vol. 22, Academic Press, New York, 1976, p. 104-107.
5.6 Block, E., Penn, R.E., and Revelle, L.K., J. Am. Chem. Soc., 1979, p. 2200.
5.7 Personal observation by M.E. Eide 5/2/83.
5.8 Alkins, H.B., "Documentation of TLVS11, American Conference of Governmental Hygienists, Cincinnati, OH, 1980, p. 13.
5.9 Toxicology Data Bank, online database from National Library of Medicine.
5.10 Weast, R. Ed., "Handbook of Chemistry and Physics", 62nd Edition, CRC Press, Boca Raton, Florida, 1981, p. C-275.
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