Beginning in the summer of 2003, a survey of Antietam's fish will be conducted in cooperation with Frostburg State University. The survey goals include determining the distributions and densities of fish in the park, establishing a baseline for continued health and population monitoring, obtaining an idea of the health of the streams based on fish populations and collecting preliminary water chemistry and physical habitat data. The Park Service plans on using the information collected as part of a larger effort to help develop a management plan for aquatic resources in the region. Up until now, only the following species have been documented in the park:

Brown Bullhead
American Eel
Central Stoneroller
White Sucker
Checkered Sculpin
Spotfin Shiner
Carp Greenside
Darter Tessellated
Darter Northern
Hogsucker Green
Sunfish Bluegill
Common Shiner
Smallmouth Bass
Largemouth Bass
Redhorse Sucker
Rosyface Shiner
Rainbow Trout
Yellow Perch
Bluntnose Minnow
Blacknose Dace
Longnose Dace
Brown Trout
Brook Trout
Creek Chub