![[logo] US EPA](https://webarchive.library.unt.edu/eot2008/20090509040751im_/http://www.epa.gov/epafiles/images/logo_epaseal.gif){kind=logo}
Cryptosporidium Image Sequence 1
Play this Sequence
Photo Credit: H.D.A. Lindquist, U.S. EPA
Photo Credit: H.D.A Lindquist, U.S. EPA
Above: Differential interference contrast (DIC) image of Cryptosporidium
parvum oocysts, purified from murine fecal material. Oocysts are spheroidal
objects, 4 to 6 microns in diameter. Oocysts may contain as many as 4
banana shaped sporozoites, and a residual body. In some oocysts the sporozoites
and residuum will be indistinct. Scale bar is 10 microns.
(higher resolution image)
Photo Credit: H.D.A Lindquist, U.S. EPA
Above: Immunofluorescence image of Cryptosporidium parvum
oocysts, purified from murine fecal material. (Same field of view) Oocysts
were stained with commercially available immunofluorescent antibodies.
Oocysts should have an intense apple green fluorescence on the periphery
of their oocyst wall, and measure 4 to 6 microns in diameter. Scale bar
is 10 microns. (higher resolution
image)
Photo Credit: H.D.A Lindquist, U.S. EPA
Above: Fluorescence image of Cryptosporidium parvum oocysts, purified from murine fecal material. (Same field of view.) Oocysts were stained with 4,6-diamidino 2-phenyl-indole dihydrochloride (DAPI). DAPI interacts with nucleic acids and stains the nucleus of each sporozoite within the oocyst. There should be 4 sporozoites each with 1 nucleus, or 4 stained nuclei in each oocyst. Oocysts that appear to have fewer than 4 stained nuclei, may have 4 nuclei with the others not visible in this plane of focus. Oocysts with no nuclei visible, may be dead, may be resistant to DAPI staining, or may be organisms other than C. parvum. (higher resolution image)
Photo Credit: These images are in the public
domain and are thus free of any copyright restrictions. For public domain
images, permission is not required for their use but we do ask that
you credit the EPA and the contributors (found in the Photo Credit line)
whenever an image is used in any publicly distributed media.
View Another Field
Other Mugshots