Design and Synthesis of CO2-Soluble Affinity Ligands for Use in CO2 Extraction of Proteins
EPA Grant Number: R824730Title: Design and Synthesis of CO2-Soluble Affinity Ligands for Use in CO2 Extraction of Proteins
Investigators: Beckman, Eric J. , Russell, Alan J.
Institution: University of Pittsburgh - Main Campus
EPA Project Officer: Karn, Barbara
Project Period: October 1, 1995 through September 30, 1998
Project Amount: $150,000
RFA: Technology for a Sustainable Environment (1995)
Research Category: Pollution Prevention/Sustainable Development
Description:
This proposal involves the design of highly CO2-soluble ligands for the extraction of molecules of biological origin. Carbon dioxide is a relatively benign solvent, and one where recovery of solutes from solution is easily accomplished via depressurization. Unfortunately, CO2 is a low dielectric fluid, and thus will not solubilize polar/hydrophilic molecules such as are commonly generated in biotechnical processes. Further, CO2 is a poor solvent for many commonly used extractant molecules, such as sufactants and chelating agents. Thus, this proposal focuses on the design of extractants/ligands particularly for use in CO2, to allow extension of CO2 to processes where its use is currently untenable. This is a jointly funded NSF/EPA project. In the EPA part of the project, we are focusing on the design of CO2-soluble chiral mobile phase agents (CMPA's) for the separation of enantiomers of ibuprofen and naproxen. A well-designed CMPA interacts specifically with one enantiomer of a racemic mixture, and thus allows separation, often times via salt formation and thus precipitation. Our goal is to combine the selectivity of a CMPA with the pressure-sensitive phase behavior inherent to CO2-based solutions to increase selectivity in a CMPA-moderated separation. Publications and Presentations:Publications have been submitted on this project: View all 10 publications for this project
Journal Articles:Journal Articles have been submitted on this project: View all 2 journal articles for this project
Supplemental Keywords:Carbon-dioxide-soluble affinity ligands; biological extraction; separation of enantiomer; racemic target molecules; chiral mobile phase ligands; carbon-dioxide-philic tails; fluoroalkyl and fluoroether. , Sustainable Industry/Business, Scientific Discipline, RFA, Technology for Sustainable Environment, Sustainable Environment, Ecological Risk Assessment, cleaner production/pollution prevention, Environmental Chemistry, cleaner production, ligands, environmentally benign solvents, depressurization, proteins, bilogical extraction, chiral mobile phase agents, carbon dioxide extraction
Progress and Final Reports:
Final Report