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Macroinvertebrate Sampling Component Information

Macroinvertebrate Database Browser
This page was created on 5-10-2009 .
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     MACROINVERTEBRATE LIABILITY DISCLAIMER AND FORMAT DESCRIPTION             

                              Disclaimer                                       

These data have been generated by State agencies under contract with the       
Department of the Interior and the Upper Midwest Environmental Sciences Center 
(UMESC). Although these data have been processed successfully on a computer    
system at the UMESC, no warranty, expressed or implied, is made regarding the  
accuracy or utility of the data on any other system or for general or          
scientific purposes, nor shall the act of distribution constitute any such     
warranty. Error correction is a continuing process, and UMESC corrects data    
data errors routinely when a correct value can be determined. UMESC does not   
delete questionable data when the correct value cannot be determined, but      
attempts to mark such data with an appropriate flag. Users are strongly        
cautioned to check the accuracy of any data obtained and to report suspected   
values to UMESC.  Methods, purposes, and conditions can vary dramatically      
among data-collecting efforts and these data should not be combined with other 
datasets from the UMESC or other sources unless the user fully understands the 
procedures and purposes used to create each set of data and can verify that    
data are comparable across sources.                                            

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                   MACROINVERTEBRATE TREND ANALYSIS DATA                       

Annual macroinvertebrate monitoring on the Upper Mississippi River System      
(UMRS) was implemented in 1992 under the Long Term Resource Monitoring Program 
(LTRMP).  Mayflies (Ephemeridae), fingernail clams (Sphaeriidae), and the      
exotic Corbicula species were selected for monitoring.  Midges (Chironomidae)  
were added to the monitoring scheme in 1993, with the addition of zebra        
mussels (Dreissena polymorpha) in 1995.   Mayflies, fingernail clams, and      
midges, found in the soft-sediment substrate, were chosen because they play an 
important ecological role in the UMRS.  The exotic Corbicula species and zebra 
mussel were chosen for sampling because of possible detrimental impacts they   
may have on the economy and biology of the UMRS.                               

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                                 FOREWARD                                      

Use of LTRMP Trend Data for Macroinvertebrate Monitoring                       

The sampling design for macroinvertebrates is relatively simple; however, all  
potential users of these data are strongly encouraged to read and understand   
the Macroinvertebrate Monitoring section of the LTRMP Procedures Manual (Thiel 
and Sauer 1999), which describes current procedures, before undertaking any    
analysis or interpretation of data.  This document is accessible by request    
from:                                                                          

          Macroinvertebrate Coordinator                                        
          Upper Midwest Environmental Sciences Center                          
          U.S. Geological Survey                                               
          2630 Fanta Reed Road                                                 
          La Crosse, WI  54603                                                 
          USA                                                                  
          Tel: (608) 783-7550, ext. 64                                         
          Fax: (608) 783-8058                                                  

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                                  SUMMARY                                      

LTRMP Macroinvertebrate Sampling Design                                        

The LTRMP conducts annual monitoring of macroinvertebrates in six study areas  
of the Upper Mississippi River System:  Navigation Pools 4, 8, 13 and          
26, and an unimpounded reach near Cape Girardeau, Missouri, on the Upper       
Mississippi River; and La Grange Pool on the Illinois River.  The LTRMP        
conducts highly standardized macroinvertebrate sampling in main channel        
border, side channel, contiguous (to the main channel or side channels)        
backwater, and impounded aquatic areas in each study areas.  Sampling is       
conducted using a standard Ponar grab dredge (Wildlife Supply Company,         
Saginaw, Michigan).                                                            

Sampling is based on a stratified random design and is conducted at            
approximately 125 sites in each study reach.  The aquatic areas serve a        
strata.  Sites include a few locations where benthic samples were collected    
historically.  Each field station is required to conduct macroinvertebrate     
sampling at the same time each year, prior to mayfly emergence.                

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                                REFERENCES                                     

Thiel, P. A., and J. S. Sauer.  1999.  Long Term Resource Monitoring Program   
Procedures:  Macroinvertebrate Monitoring.  U.S. Geological Survey,            
Upper Midwest Environmental Sciences Center, La Crosse, Wisconsin,             
Revised May 1999. LTRMP 95-P002-2 (Revised 1999) 7pp. + Appendixes A-H.        

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                INVERTEBRATE FIELD FORMATS                       


1992 DATA OR EARLIER IS SORTED BY DATE, SAMPLE GRID, UTM EASTING,              
AND UTM NORTHING IN THAT ORDER.                                                
1993 DATA OR LATER IS SORTED BY BARCODE ONLY.                                  

THE "FORMAT" COLUMN CONTAINS THE FOLLOWING FORMAT SPECIFIERS:                  
                       "a": alpha character (always UPPER case);               
                       "b": a blank space;                                     
                   "blank": a missing value;                                   
                       "c": alpha character/symbol/digit;                      
                   "c(xx)": character string where xx represents the maximum   
                            numberof characters allowed;                       
                       "9": any digit, 0 - 9;                                  
              "MM/DD/YYYY": date format;                                       
                   "HH:MI": time format;                                       


Field NameField SizeValue Range
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BARCODE899999999
SAMPLE GRID599999
DATE10MM/DD/YYYY
FIELD STATION NUMBER19
HABITAT CLASS5aaa-a
RIVER MILE5c99.9
SUBSAMPLE TAKEN1c
UTM ZONE(UMESC)299
UTM EASTING (UMESC)6999999
UTM NORTHING (UMESC)79999999
DEPTH499.9
CONTAINERS299
SUMMARY CODE19
GEAR CODE1a
LAB SAMPLE TAKEN1a
% SUBMERSED/FLOATING LEAF VEG (Ponar)19
PREDOMINANT SUBSTRATE19
SHELLS1c
DETRITUS1c
FINE ORGANIC MATERIAL1c
SUBMERSED VEGETATION19
FLOATING LEAF19
EMERGENT VEGETATION19
OPEN WATER19
MAYFLIES49999
FINGERNAIL CLAMS49999
CORBICULA49999
MIDGES49999
ZEBRA MUSSELS49999
DRAGONFLIES DAMSELFLIES1a
STONEFLIES1a
CADDISFLIES1a
AQUATIC FLIES1a
FRESHWATER MUSSELS1a
AQUATIC WORMS LEECHES1a
FRESHWTR SHRIMP CRAYFISH1a
SCUDS1a
SNAILS1a
COMMENTS80a(80)
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HISTORICAL SUMMARY

FIELD NAMEYEARS USED
--------------------------------
BARCODE1993 TO 2004
SAMPLE GRID1992 TO 2004
DATE1992 TO 2004
FIELD STATION NUMBER1992 TO 2004
HABITAT CLASS1992 TO 2004
RIVER MILE1993 TO 2004
SUBSAMPLE TAKEN1994 TO 2004
UTM ZONE (UMESC)1992 TO 2004
UTM EASTING (UMESC)1992 TO 2004
UTM NORTHING (UMESC)1992 TO 2004
DEPTH1992 TO 2004
CONTAINERS1993 TO 2004
SUMMARY CODE1992 TO 2004
GEAR CODE1992 TO 2004
LAB SAMPLE TAKEN1992 TO 2004
%SUBMERSED/FLOATING LEAF VEG (Ponar)1992 TO 2004
PREDOMINANT SUBSTRATE1992 TO 2004
SHELLS1993 TO 2004
DETRITUS1993 TO 2004
FINE ORGANIC MATERIAL1993 TO 2004
SUBMERSED VEGETATION1992 TO 2004
FLOATING LEAF1992 TO 2004
EMERGENT VEGETATION1992 TO 2004
OPEN WATER1992 TO 2004
MAYFLIES1992 TO 2004
FINGERNAIL CLAMS1992 TO 2004
CORBICULA1992 TO 2004
MIDGES1993 TO 2004
ZEBRA MUSSELS1995 TO 2004
DRAGONFLIES DAMSELFLIES1998 TO 2004
STONEFLIES1998 TO 2004
CADDISFLIES1998 TO 2004
AQUATIC FLIES1998 TO 2004
FRESHWATER MUSSELS1998 TO 2004
AQUATIC WORMS LEECHES1998 TO 2004
FRESHWATER SHRIMP CRAYFISH1998 TO 2004
SCUDS1998 TO 2004
SNAILS1998 TO 2004
COMMENTS1992 TO 2004
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FIELD DESCRIPTIONS


BARCODE
Identifying barcode for invertebrate data sheets.

SAMPLE GRID
Sample grid area for a collection site. Grid numbers are unique descriptors
for each sample site location, and are derived from a pre-determined UTM
grid system developed at UMESC.

DATE
The month, day, and year the site was sampled. Format is mm/dd/yyyy.

FIELD STATION NUMBER
Numeric code identifying the field station number. Listed below are the
valid codes and their corresponding field station.
1 = Lake City, MN 4 = Brighton, IL
2 = Onalaska, WI 5 = Jackson, MO
3 = Bellevue, IA 6 = Havana, IL

HABITAT CLASS
Alphabetic code LTRMP habitat class description.
Valid codes are as follows:
BWC = Backwater contiguous
IMP = Impounded
MC = Main channel
MCB = Main channel border
SC = Side channel
TDL = Tributary delta lake (Lake Pepin only)

RIVER MILE
The location of the site to the nearest tenth of a river mile.

SUBSAMPLE TAKEN
A Code describing if a subsample was taken
Valid codes are as follows:
1994 TO 1996 only A = Large, Subsample(268 square centimeteters)
B = Small, Subsample(162 square centimeteters)
N = No, Subsample Taken
1997 - TO PRESENT Y = Yes, Subsample Taken
N = No, Subsample Taken

UTM ZONE (UMESC)
Numeric field to record Global Positioning System (GPS) zone. LTRMP data
resides in either zone 15 or 16.
This zone is derived at UMESC and is then used by the collection crews
to locate where they are to do their sampling.

UTM EASTING (UMESC)
East UTM (Universal Transverse Mercator) coordinates of collection sites.
This coordinate is derived at UMESC and is then used by the collection
crews to locate where they are to do their sampling.

UTM NORTHING (UMESC)
North UTM (Universal Transverse Mercator) coordinates of collection sites.
This coordinate is derived at UMESC and is then used by the collection
crews to locate where they are to do their sampling.

DEPTH
Water depth recorded in meters.

CONTAINERS
The total number of sample containers collected at the site and taken back to
the lab (replaced # containers per replicate A, B, C in 1993).

SUMMARY CODE
A numeric code representing whether or not the sample was kept and the
circumstances surrounding the decision to accept or reject. Valid codes
are as follows:
1 = Sample taken
2 = Sample taken but not kept due to hard substrate
3 = Sample taken but not kept due to detritus, shells, etc.
4 = Other

GEAR CODE
A code representing the gear type used to collect the sample.
(P = Standard Ponar).
(E = Petite Ponar)

LAB SAMPLE TAKEN
Code if the sample was chosen for lab analysis. Valid codes are as
follows:
Y = Yes, sample chosen for lab analysis.
N = No, sample not chosen for lab analysis.

% SUBMERSED AND FLOATING LEAF VEGETATION (Ponar)
This visual observation is a subjective measurement based on
the column of water and sediment the Ponar falls through. A
numeric code is recorded representing the percentage
of submersed and floating-leaf vegetation.
This field will provide data on whether or not invertebrates
associated with vegetation are in the sample and the relationship of
sites with and without vegetation.
0 = 0%
1 = 1%-20%
2 = 21%-50%
3 = 51%-90%
4 = 91%-100%

PREDOMINANT SUBSTRATE
A code to represent the qualitative observation of
sediments based on visual and tactile observations at the site.
Valid codes are as follows:
1 = Hard clay
2 = Silt/Clay
3 = Mostly silt/clay with sand
4 = Mostly sand
5 = Sand
6 = Gravel/rock

SHELLS
A code representing the percentage of dead shells in the sample
wash frame. Valid codes are as follows:
0 = 0%
1 = 1%-20%
2 = 21%-50%
3 = 51%-90%
4 = 91%-100%

DETRITUS
A code representing the percentage of detritus (fibrous
material on the surface and within the sediment) in the sample
wash frame. Valid codes are as follows:
0 = 0%
1 = 1%-20%
2 = 21%-50%
3 = 51%-90%
4 = 91%-100%

FINE ORGANIC MATERIAL
A code indicating if fine organic material (characterized by black
coloration and odor) is present in the sample.
Valid codes are as follows:
Y = Yes, fine organic material present
N = No, fine organic material not present

SUBMERSED VEGETATION
A code representing the percentage of submersed vegetation
within a 15-m radius from the boat. Valid codes are as follows:
0 = 0%
1 = 1%-20%
2 = 21%-50%
3 = 51%-90%
4 = 91%-100%

FLOATING LEAF
A code representing the percentage of floating leaf vegetation
within a 15-m radius from the boat. Valid codes are as follows:
0 = 0%
1 = 1%-20%
2 = 21%-50%
3 = 51%-90%
4 = 91%-100%

EMERGENT VEGETATION
A code representing the percentage of emergent vegetation
within a 15-m radius from the boat. Valid codes are as follows:
0 = 0%
1 = 1%-20%
2 = 21%-50%
3 = 51%-90%
4 = 91%-100%

OPEN WATER
A code representing the percentage of open water
within a 15-m radius from the boat. Valid codes are as follows:
0 = 0%
1 = 1%-20%
2 = 21%-50%
3 = 51%-90%
4 = 91%-100%

MAYFLIES
The number of mayfly larvae found in the collection sample for
the site.

FINGERNAIL CLAMS
The number of fingernail clams found in the collection sample for
the site.

CORBICULA
The number of corbicula found in the collection sample for
the site.

MIDGES
The number of midges found in the collection sample for
the site.

ZEBRA MUSSELS
The number of zebra mussels found in the collection sample for
the site (not started recording until 1995).

DRAGONFLIES DAMSELFLIES
A code representing if dragonflies or damselflies are absent (A) or
present (P) in the sample (not started recording until 1998).

STONEFLIES
A code representing if stoneflies are absent (A) or
present (P) in the sample (not started recording until 1998).

CADDISFLIES
A code representing if caddisflies are absent (A) or
present (P) in the sample (not started recording until 1998).

AQUATIC FLIES
A code representing if aquatic flies are absent (A) or
present (P) in the sample (not started recording until 1998).

FRESHWATER MUSSELS
A code representing if fresh water mussels are absent (A) or
present (P) in the sample (not started recording until 1998).

AQUATIC WORMS LEECHES
A code representing if aquatic worms or leeches are absent (A) or
present (P) in the sample (not started recording until 1998).

FRESHWATER SHRIMP CRAYFISH
A code representing if fresh water shrimp or crayfish are absent (A) or
present (P) in the sample (not started recording until 1998).

SCUDS
A code representing if scuds are absent (A) or
present (P) in the sample (not started recording until 1998).

SNAILS
A code representing if snails are absent (A) or
present (P) in the sample (not started recording until 1998).

COMMENTS
Eighty-character field to record miscellaneous comments and
observations.
Last updated on: January 13, 2005
Contacting the Upper Midwest Environmental Sciences Center
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