|
Biological Characteristics
Species
|
Hyla
regilla (sometimes listed as Pseudacris regilla) is a small frog measuring from ¾ to 2
inches with a distinctive black or dark brown eye stripe and notable toe
pads (Stebbins, 1985). A darker triangle is apparent between the eyes
(Wright and Wright, 1995). The dorsal colors vary from brown to green,
as well as combinations of brown and green, with a series of dark spots
that may be more or less distinctive depending on the color phase (Stebbins,
1985). Ventral color is cream colored and leg area may be yellowish to
orange (Wright and Wright, 1995). Males have dusky, wrinkled throats (Behler
and King, 2000). Eyes from the pupil rim behind and in front is broken
by black; above the rim is lemon yellow, while the rest of the iris is
raw sienna or antique brown (Wright and Wright, 1995). |
Nesting
and Status in Estuarine and Coastal Areas |
The
habitat of the Pacific treefrog varies from sea level to mountains
(11,600 ft) and includes grassland, chaparral, woodland, forest, desert,
oases and farmland (Stebbins, 1985). Pacific treefrogs breed from
November to July in marshes, lakes, ponds, roadside ditches, reservoirs
and slow streams (Stebbins, 1985). The brown and yellowish
eggs, in small, loose, irregular masses (10-70 eggs) are laid in quiet
water beneath or sometimes at the surface, attached to vegetation; a
total of 500 to 750 eggs can be deposited from a single female (Wright
and Wright, 1995). Tadpoles transform after 50 to 80 days (Wright and
Wright, 1995). Lifespan is unknown for the Pacific treefrog, but 2 years
are required from hatching to maturity (Stebbins, 1951). |
Abundance
and Range |
The
Pacific treefrog is probably the most abundant frog found in California
(Wright and Wright, 1995). Pacific treefrogs can be found from Mt.
Scriven and McBride in British Columbia all the way south to the tip of
Baja California, and from the Pacific coast eastward to western Montana
and eastern Nevada (Stebbins, 1985). These are the only native frogs
known to inhabit the Channel Islands off the coast of southern
California. |
Site
Fidelity |
Although
this frog frequents ponds, springs, streams, irrigation canals and other
bodies of water, it has been found as far as one-half mile from water (Stebbins,
1951). |
Ease
of Census |
Moderate
effort is required to expose and census egg masses. Census of breeding
frogs entering and exiting pools or of calling frogs in the breeding
season is relatively easy. |
Feeding
Habits |
Pacific
treefrogs eat a variety of small aquatic and terrestrial invertebrates.
An examination of stomach contents of 18 individuals revealed a diet
primarily of small leaf hoppers (jassids)
and various dipterans
(including midges, small crane flies, and muscoid flies), but also
included ants, spiders (Erigone),
parasitic hymenopterans,
and small beetles (Stebbins, 1951). |
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|
I.
|
Organochlorine Contaminants |
A.
|
Concentrations
in Adults and Juveniles |
1.
|
Tadpoles
were collected from two sites in California during June and July 1996 (Datta
et al., 1998). The following are the composite concentrations (ng/g wet
weight) found: Sycamore
Creek, Southern Sierra Nevada mountains, n = 31, June 22, (PCBs
= 12 / DDE = 9.6); Sycamore
Creek, Southern Sierra Nevada mountains, n = 44, July 9, (PCBs
= 23.8 / DDE = 9.3); UC
Davis, n=32, July 2, (PCBs
= 229.3 / DDE = 258 ). |
2. |
Composite
samples of adults (n=48) and tadpoles (n=34) were collected at various
California sites (Sparling et al., 2001). The following concentrations (ng/g)
are listed as (mean / percent detected ):
Coastal California (reference site): Endosulfans
(1.8 / 28%); Lassen: Endosulfans
(1.0 / 12%); Tahoe: SDDT
(1 / 18), Endosulfans (4.5
/ 82%); SHCH
(0.8 / 5%); Yosemite: SDDT
(2.2 / 40), Endosulfans
(2.1 / 67%); SHCH
(0.9 / 11%); Sequoia:
SDDT
(6.9 / 30), Endosulfans
(3.1 / 67%); SHCH
(0.9 / 10%). Among all the pesticides tested SDDT
and endosulfans had the
highest frequency of occurrence across locations. For adults and
tadpoles collectively the difference among locations was significant.
The contaminant HCH
was only found in adults. |
3. |
Pooled
tadpole samples from the Sierra Nevada mountains in California were
analyzed for SPCB
and Stoxaphene
(Angermann et al., 2002). The
following mean concentrations are listed in order of (SPCB
ng/g, toxaphene ng/g):
Auburn Slate Rec Area (243.75, 15.62); French Creek (12.26, 3.88);
DeLong Creek (33.91, 10.03);
California Youth Authority Pond (12.02, 7.96);
Alpha Diggings (14.51, 8.6);
Big Meadow (22.15, 2.37); Secret
Diggings (22.97, 5.76); Miguel
Meadow (6.75, 3.81); Camp
Spaulding Pond (35.28, 9.15); Loch
Lleven Pond (18.98 / NA); Crane
Flat Meadow (10.31, 2.05); Pacific
Crest Trail at I-80 (NA / 4.72); Siesta
Lake (22.77, NA); Margery
Pond (20.89, NA); Crooked
Meadow (14.70, NA); Kaiser
Pass Meadow (1.57, 2.88); Pear
Lake (5.69, 2.02); Pond at
Bennettville (7.97, 3.35); Pond
at Tioga Pass (4.36, 2.28); Sixty Lakes Basin (2.55,
1.47); Mt. Conness
Meadow (4.21, 2.23). Both toxaphene
and PCB concentrations generally diminished with increasing
elevations. Neither latitude nor Central Valley (a source of pesticides)
to site distance correlated with PCB
or toxaphene residues in
multiple stepwise regression analysis, yet both were significant when
regressed independently against S toxaphene residue concentrations. A comparison of sites
designated within east-facing catchment basins with sites designated
within west-facing (towards the central valley) catchment basins yielded
significant different mean concentrations of both SPCB
and S
toxaphene supporting a rain-shadow model of residue
disposition in mountainous zones. West
facing sites at the northern end of the sampling range, in the northern
Sierra Nevada mountains, had relatively high proportions of low and
moderately chlorinated congeners compared to sites in the central Sierra
Nevada mountains and at the southern end of the sampling range. |
B.
|
Concentrations
in Eggs |
1. |
Egg
masses (n = 8) were collected from Upper Meadow in the northern Sierra
Nevada mountains, California on July 11, 1996 contained 9.1 ng/g wet
weight PCBs (Datta et al.,
1998). |
II.
|
Cholinesterase-Inhibiting Pesticides |
1. |
Pacific
treefrog tadpoles were collected from two sites in California during
June and July 1996 (Datta et al., 1998). Mean concentrations of chlorpyrifos
(ng/g wet weight) were 10.4 (June, n=31) and 17.4 (July, n=44) at
Sycamore Creek, Southern Sierra Nevada mountains, and 9.2 (July, n=32)
at UC Davis. Egg masses
collected from the Upper Meadow in the northern Sierra Nevada mountains
contained trace amount of chlorpyrifos
(July, n=8). |
2. |
Tongues
and brain tissue from adult Pacific treefrogs collected from various
sites in California were compared for cholinesterase
(ChE) activity (nmol substrate hydrolyzed/min/g tissue) after
exposure to diazinon (9 mg/g)
for 5 days in a laboratory setting (Sparling et al., 2001). The mean ChE
activities were as follows: Tongues control group (0.934);
Tongues diazanon
exposed group (0.572); Brain control group (5.723); Brain diazanon
exposed group (5.95). Whole
body composites of tadpoles (n=170) taken from 23 sites in California
were analyzed for ChE
activity and compared by development and by site as follows: Tadpoles
with joined hind limbs (2.38); tadpoles with limb bud (1.69); tadpoles
with no limb (1.69). Tadpole
ChE was depressed at both
Lake Tahoe and Sequoia compared with coastal sites and was less at
Sequoia than at Lake Tahoe. Composites
of adults (n=48) and tadpoles (n=34) were collected from various
California sites. The following concentrations (ng/g) are listed as
(mean / percent detected): Coastal
California (reference site): Chloropyrifos
(1 / 9%); Lassen: Chloropyrifos
(1 / 29%); Tahoe: Chloropyrifos (1.5 / 22%), Diazanon
(2 / 11%); Yosemite:
Chloropyrifos (14 /
12%), Diazanon (3 / 53%);
Sequoia: Chloropyrifos
(8 / 16%), Diazanon (2 /
9%). More than 50% of tadpoles and adults at Yosemite Park had
measurable concentrations of chlorpyrifos
or diazinon, both potent ChE
inhibitors, while the
reference site had only 9% with measurable concentrations of these
compounds. |
III.
|
Trace Elements, Metals, and Metalloids |
|
No
residue data available |
IV.
|
Petroleum |
|
No
residue data available |
V. |
Other |
1.
|
Tadpoles
were collected from two sites in California during June and July 1996 (Datta
et al., 1998). Mean concentrations of chlorothalonil
(ng/g wet weight) were 47.7 (June, n=31) and 33.3 (July, n=44) Sycamore
Creek, Southern Sierra Nevada mountains, and 12.7 (July l, n=32) at UC
Davis. Egg masses collected
from the Upper Meadow in the northern Sierra Nevada mountains contained
4.5 ng/g chlorothalonil
(July, n=8). |
|
|
Pacific
Treefrog Contaminant Response Data |
|
I.
|
Organochlorine Contaminants |
|
No
response data available |
II.
|
Cholinesterase-Inhibiting Pesticides |
1. |
Three-week-old
tadpoles hatched in the laboratory from egg masses collected from ponds
near Concord, California were exposed to field application
concentrations of five organophosphorus pesticides (Johnson, 1980).
After 24 exposure periods, tadpoles were tested for thermal stress by
increasing ambient water temperature until spasms were noted. The
following data are listed as (concentration in ppb, average
temperature at start of spasm in degrees Centigrade, number in sample):
Chloropyrifos (25, 35.3,
n=36), (50, 34.1, n=30); Methyl parathion (25, 35.7, n=27), (50, 34.3, n=34), (100,
33.6, n=36); Temephos
(25, 36.2, n=39), (50, 35.5, n=37);
Fenthion (25, 36.9, n=42), (50, 36.7, n=35), (100, 35.7,
n=45); Malathion (25, 36.9, n=28), (50, 36.4, n=31), (100, 36.4,
n=35), (500, 36.1, n=27). The thermal tolerance was significantly
lowered by all chemicals and concentrations tested. The most active
toxicants were chloropyrifos and
methyl parathion, while malathion
was found to be the least toxic chemical to the tadpoles. Mortality was
observed with exposure to chloropyrifos
(6 dead at 50 ppb) and methyl
parathion (1 dead at 50 ppb). |
III.
|
Trace Elements, Metals, and Metalloids |
|
No
response data available |
IV.
|
Petroleum |
|
No
response data available |
V. |
Other |
1. |
Embryos
(stage 12) produced from egg masses collected within the Willamette
Valley in Oregon were exposed (4 and 10 day tests) to increasing
concentrations of ammonium compounds
and sodium nitrate (NaNO3)
(Schuytema and Nebecker, 1999). During the 10 day test, in addition to a
control set of embryos exposed only to well water, sets of embryos were
also exposed to a sodium chloride (NaCl)
solution with a Cl equivalent to a 100 mg/L ammonium
chloride (NH4Cl)
solution and to a sulfate solution
with an SO4 equivalent to a 100 mg/L ammonium
sulfate ((NH4)2SO4)
solution. The mortality rates (increasing concentrations in mg/L / %
embryos that died) were as follows:
Four day test :
Ammonium nitrate (NH4NO3),
(0.3 mg/L / 0 %, 3.3
/ 0, 6.9 / 0,
13.3 / 0, 25.1 / 0, 50.9
/ 80, 101.2 / 100); NH4Cl
(0.2 / 3.3, 2.8
/ 0, 7.3 / 0, 12.8 / 0,
24.9 / 6.7, 49.7 /
23.3, 102.9 / 96.7); (NH4)2SO4
(0.04 / 0,
2.6 / 0, 6.1 / 0,
11.7 / 0, 23.1 / 0, 45.4 / 0, 91.5 / 43.3);
NaNO3 (0.1
/ 0, 24.8 / 0,
56.7 / 0, 111 / 0,
230.4 / 16.7, 470.4 / 43.3, 979.2
/ 56.7, 2,716 / 100).
Ten day test: NH4NO3,
(0.3 mg/L / 0 %, 3.3
/ 0, 6.9 / 0, 13.3 / 6.7,
25.1 / 46.7, 50.9 / 100,
101.2 / 100); NH4Cl (0.2
/ 3.3, 2.8 / 0, 7.3 / 0,
12.8 / 0, 24.9 /
23.3, 49.7 / 100,
102.9 / 100); (NH4)2SO4
(0.04 / 0,
2.6 / 0, 6.1 / 0,
11.7 / 3.3, 23.1 /
3.3, 45.4 / 93.3, 91.5 / 100);
NaNO3 (0.1
/ 0, 24.8 / 0,
56.7 / 0, 111 / 0,
230.4 / 20, 470.4 /
46.7, 979.2 / 63.3, 2,716
/ 100). Embryos were
smaller and exhibited less spontaneous movement than controls after 2 to
3 days exposure to 50-100 mg/L (NH4NO3)or
1000 mg/L NaNO3,
and embryos appeared to be immobilized by 1000 mg/L NaNO3. No embryos died when Cl
and SO4
were held at the same levels as test solutions of ammonium or chloride
sulfate at 100 mg/L
concentrations. No differences were found in length or weight between
embryos exposed to these ions or to a well water control. The
LC50 (median lethal concentration) values (mg/L) for these embryos (4
day test / 10 day test) were: NH4NO3
(41.1 / 25); NH4Cl (60.3
/ 30.3); (NH4)2SO4
(>102.9 / 32.4); NaNO3
(643 / 578). The LOAEL (lowest observed adverse effect level)
and NOAEL (no observed adverse effect level) values (mg/L) using the 10
day exposure results (LOAEL / NOAEL) were:
NH4NO3
(6.9 / 3.3); NH4Cl (24.9
/ 12.8); (NH4)2SO4
(11.7 / 6.1); NaNO3
(111 / 56.7). |
|
|
Angermann,
J.E., G.M. Fellers, and F. Matsumura. 2002. Polychlorinated biphenyls
and toxaphene in Pacific Tree Frog tadpoles (Hyla
regilla) from the California Sierra Nevada, USA. Environ.
Toxicol. Chem. 21:2209-2215. |
|
Behler,
J.L. and F.W. King. 2000. National
Audubon Society Field Guide to North American Reptiles and Amphibians.
Alfred A. Knopf. New York. 743 pp. |
|
Datta,
S., L. Hansen, L. McConnell, J. Baker, J. LeNoir, and J.N. Selber. 1998.
Pesticides and PCB contaminants in fish and tadpoles from the Kaweah
River Basin, California. Bull. Environ. Contam. Toxicol. 60:829-836. |
|
Johnson,
C.R. 1980. The effects of five organophosphorus insecticides on thermal
stress in tadpoles of the Pacific treefrog, Hyla
regilla. Zoological Journal of the Linnean Society
69:143-147. |
|
Schuytema,
G.S. and A.V. Nebecker. 1999. Comparative effects of ammonium and
nitrate compounds on Pacific treefrog and African clawed frog embryos.
Arch. Environ. Contam. Toxicol. 36:200-206. |
|
Sparling,
D.W., G.M. Fellers, and L.L. McConnell. 2001. Pesticides and amphibian
population declines in California, USA.
Environ. Toxicol. Chem. 20:1591-1595. |
|
Stebbins,
R.C. 1951. Amphibians of Western
North America. University of California Press, Berkeley. 539
pp. |
|
Stebbins,
R.C. 1985. Peterson Field Guide to
Western Reptiles and Amphibians. Houghton Mifflin Co. Boston.
336 pp. |
|
Wright,
A.H. and A.A. Wright. 1995. Handbook
of Frogs and Toads of the United States and Canada. Comstock
Publishing Associates. Ithaca, NY. 640 pp. |