Cryosurvival of Embryos From Dysmorphic Oocytes - Full Text View

Sponsored by:	V.K.V. American Hospital, Istanbul
Information provided by:	V.K.V. American Hospital, Istanbul
ClinicalTrials.gov Identifier:	NCT00521443

Purpose

Various morphological abnormalities of human oocytes were reported to detrimentally affect embryo development. We observed development of frozen thawed embryos derived from oocytes with normal or abnormal morphological features to the blastocyst stage. Cytoplasmic abnormalities of the oocytes were found to negatively affect cryosurvival potential of the embryos.

Condition	Intervention
Cryopreservation Blastocyst	Procedure: Cryopreservation, thawing and blastocyst culture

U.S. FDA Resources

Study Type:	Observational
Study Design:	Natural History, Longitudinal, Defined Population, Prospective Study

Further study details as provided by V.K.V. American Hospital, Istanbul:

Groups/Cohorts	Assigned Interventions
Normal: Control Embryos derived from morphologically normal MII oocytes	Procedure: Cryopreservation, thawing and blastocyst culture Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.
Irregular Shapes: Case Embryos derived from irregularly shaped oocytes.	Procedure: Cryopreservation, thawing and blastocyst culture Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.
Large PVS: Case Embryos derived from oocytes with large perivitelline space.	Procedure: Cryopreservation, thawing and blastocyst culture Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.
Dark Zona: Case Embryos derived from oocytes with dark zona pellucida	Procedure: Cryopreservation, thawing and blastocyst culture Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.
Dark cytoplasm: Case Embryos derived from oocytes with dark cytoplasm	Procedure: Cryopreservation, thawing and blastocyst culture Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.
Vacuolar cytoplasm: Case Embryos derived from oocytes with vacuolated cytoplasm	Procedure: Cryopreservation, thawing and blastocyst culture Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.
Central granulation: Case Embryos derived from oocytes with centrally granulated cytoplasm	Procedure: Cryopreservation, thawing and blastocyst culture Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.
Double extra: Case Embryos derived from oocytes with double extracytoplasmic abnormalities	Procedure: Cryopreservation, thawing and blastocyst culture Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.
Double combined: Case Embryos derived from oocytes with any combination of one extracytoplasmic and one cytoplasmic anomaly	Procedure: Cryopreservation, thawing and blastocyst culture Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.
Double cytoplasmic: Case Embryos derived from oocytes with any two cytoplasmic anomalies	Procedure: Cryopreservation, thawing and blastocyst culture Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.
Triple extra: Case Embryos derived from oocytes with triple extracytoplasmic anomaly	Procedure: Cryopreservation, thawing and blastocyst culture Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.
Triple combined: Case Embryos derived from oocytes with triple combined anomalies	Procedure: Cryopreservation, thawing and blastocyst culture Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.

Eligibility

Ages Eligible for Study:	18 Years to 45 Years
Genders Eligible for Study:	Female

Criteria

Inclusion Criteria:

Human embryos derived from morphologically normal or abnormal mature oocytes that fertilized normally by microinjection procedure.
Embryos were obtained from couples who refused to freeze their supernumerary embryos after fresh embryo transfer in an assisted reproduction treatment cycle.

Exclusion Criteria:

Embryos obtained after microinjection to immature oocytes taht were in vitro matured in culture.
Embryos obtained from abnormally fertilized zygotes.

Contacts and Locations

Please refer to this study by its ClinicalTrials.gov identifier: NCT00521443

Locations

Turkey
VKV American Hospital
Istanbul, Turkey, 34365

Sponsors and Collaborators

V.K.V. American Hospital, Istanbul

Investigators

Study Director:

Basak Balaban, B.Sc.

Assisted Reproduction Unit of the VKV American Hospital of Istanbul

More Information

No publications provided by V.K.V. American Hospital, Istanbul

Additional publications automatically indexed to this study by National Clinical Trials Identifier (NCT ID):

Balaban B, Ata B, Isiklar A, Yakin K, Urman B. Severe cytoplasmic abnormalities of the oocyte decrease cryosurvival and subsequent embryonic development of cryopreserved embryos. Hum Reprod. 2008 Aug;23(8):1778-85. Epub 2008 May 12.

Study ID Numbers:	AH-05/04
Study First Received:	August 24, 2007
Last Updated:	August 24, 2007
ClinicalTrials.gov Identifier:	NCT00521443 History of Changes
Health Authority:	Turkey: Ministry of Health

ClinicalTrials.gov processed this record on May 07, 2009