RESEARCH INFORMATION BULLETIN NO. 1 DNA Mediated Bacterial Transformation A Bibliography, 1955-61 Syntex Institute for Molecular Biology 3221 Porter Drive Stanford Industrial Park Palo Alto, California DNA Mediated Bacterial Transformation A bibliography compiled by Martha W. West Syntex Institute for Molecular Biology 1 November 1961 An attempt has been made at comprehensive coverage of the literature on bacterial transformation during the period January, 1955-June, 1961. The main criterion for inclusion was that the material dealt with the transforming activity of DNA and/or bacteria in the process of transformation. Wherever possible, abstracts of material not available for examination (marked *) are cited. 1) Abe, M. An intermediate stage of pneumococcus transformation as revealed by the effect of 8-azaguanine. Japan J. Med. Sci. & Biol. 12:441-52, 1959. 2) Abe, M. and Mizuno, D. A contribution to the knowledge of pneumococcus transformation during the period between the incorporation of deoxyribonucleic acid and the appearance of streptomycin resistance. Biochim. et Biophys. Acta 32:464-9, 1959. 3) Alexander, H. E. and Leidy, G. Origin of species in micro- organisms: use of transformation as a tool. Am. J. Diseases I Children 90:860-l, 1955. 4) Anagnostopoulos, C. and Crawford, I. P. Linkage studies by transformation in Bacillus subtilis. Bacterial. Proc. 61:89, 1961. 5) Anagnostopoulos, C. and Crawford, I. P. Transformation studies on the linkage of markers in the tryptophan pathway in Bacillus subtilis. Proc. Nat. Acad. Sci. U.S. 47:378-90, 1961. 6) Anagnostopoulos, C. and Spizizen, J. Requirements for trans- formation in Bacillus subtilis. J. Bacterial. 81:741-6, 1961. 7) Austrian, R. and Bernheimer, H. P. Some biological properties of doubly capsulated pneumococci. J. Clin. Invest. 34:920-l, 1955. 8) Austrian, R., Bernheimer, H. P., Smith, E. E. B. and Mills, G. T. Genetic biochemistry of double encapsulation in pneumococcus. Intern. Congr. Microbial., 7th Congr., Stockholm, 1958. Abstr. p. 48. 9) Austrian, R., Bernheimer, H. P., Smith, E. E. B. and Mills, G. T. Acquisition of new capsular type by pneumococcus, a multi- factor transformation. Cold Spring Harbor Symposia Chant. Biol. 23:99-100, 1958. -2- 10) Austrian, R. and Bernheimer, H. P. Simultaneous production of two capsular polysaccharides by pneumococcus. I. Properties of a pneumococcus manifesting binary capsulation. J. Exp. Med. 110:571-83, 1959. 11) Austrian, R., Bernheimer, H. P., Smith, E. E. B. and Mills, G. T. Simultaneous production of two capsular polysaccharides by pneumococcus. II. The genetic and biochemical basis of binary capsulation. J. Exp. Med. 110:585-602, 1959. 12) Balassa, R. Durch desoxyribonucleinsguren induzierte Veranderungen an Rhizobien. Naturwiss. 42:133, 1955. 13) Balassa, R. In viva induzierte Transformationen bei Rhizobien. Naturwiss. 43:422-3, 1956. 14) *Balassa, R. Induced changes by deoxyribonucleic acids in Rhizobium. Acta Microbial. Acad. Sci. Hung. 4:77-84, 1957. CA 51:12206b, 1957. - 15) *Balassa, R. Some limitations in the formation of deoxyribo- nucleic acid-induced low streptomycin resistance by Rhizobium. Acta Microbial. Acad. Sci. Hung. 4:85-95, 1957. CA 51:12206d, 1957. - 16) Balassa, R. Transformationserscheinungen bei Rhizobien. Intern. Congr. Microbial., 7th Congr., Stockholm, 1958. Abstr., p. 49. 17) Balassa, R. Transformation of a strain of Rhizobium lupine. Nature 188:246-7, 1960. 18) Balows, A., Weaver, R. H. and Humphries, ,T. C. Attempts to demonstrate type formation with Klebsiella pneumoniae. J Bacterial. 69:139-41, 1955. -L 19) Beiser, S. M. and Ellison, S. A. The effect of ultraviolet irradiation on pneumococcus transforming factor. Intern. Congr. Microbial., 7th Congr., Stockholm, 1958. Abstr., p. 51. 20) Beiser, S. M., Pahl, H. B., Rosenkranz, H. S. and Bendich, A. Studies on the fractionation of transforming deoxyribonucleic acid of pneumococcus. I. Recovery and dose-response relations. Biochem. et. Biophys. Acta 34:497-502, 1959. 21) Bellin, J. S. and Oster, G. Photodynamic inactivation of transforming principle. Biochim. et Biophys. Acta 42:533-5, 42:533-5, 1960. 22) Bendich, A., Fresco, J. R., Rosenkranz, H. S. and Beiser, S. M. Fractionation of deoxyribonucleic acid (DNA) by ion exchange. J. Am. Chem. Sot. 77:3671-72, 1955. -3- 23) Bendich, A., Pahl, H. B. and Beiser, S. M. Chromatographic fractionation of deoxyribonucleic acid w~ith special emphasis on the transforming factor of pneumococcus. Cold Spring Harbor Symposia Cuant. Biol. 21:31-48, 1956. 24) Bendich, A., Pahl, H. B. and Brown, G. B. Chromatographic fractionation of E. coli DNA containing 5-Bromouracil-2-C14. Discussion,in Symposium on the Chemical Basis of Heredity, ed. by W. D. McElroy a&Bentley Glass. Baltimore: John Hopkins Press, 1957. Pp. 378-96. 25) Bendich, A, Pahl, H. B., Rosenkranz, H. S. and Rosoff, M. Studies of deoxyribonucleic acids with the acid of anion exchangers. Symposia Sot. Exp. 'BiaL 12:31-48, 1958. 26) Bloch, H., Walter, A. and Yamamura, Y. Failure of deoxyribo- nucleic acid from mycobacteria to induce bacterial trans- formation. Am. Rev. Respirat. Diseases 80:911, 1959. 27) Bracco, R. M., Krauss, M. R., Rot, A. S. and MacLeod, C. M. Transformation reactions between pneumococcus and three strains of streptococci. J. Exp. Med. 106:247-59, 1957. 28) Catlin, B. W. Activities of deoxyribonucleates and of a deoxyribonuclease from meningococcus. Bacterial. Proc. 59: 28-29, 1959. 29) Catlin, B. W. Transformation reactions within and between species of Neisseria. Bacterial. Proc. 60:74, 1960. 30) Catlin, B. W. Transformation of Neisseria meningitidis by deoxyribonucleates from cells and from culture slime. J. Bacterial. 79:579-90, 1960. 31) Catlin, B. culture 608-10, w. Interspecific transformation of Neisseria by slime containing deoxyribonucleate. Science 131: 1960. 32) Catlin, B. w. Affinities among Neisseria as revealed by studies of DNAs and DNases. Bacterial. Proc. 61:90, 1961. 33) Cavalieri, L. F. Paucidisperse deoxyribonucleic acid and its use in the study of genetic determinants. J. Am. Chem. Sot. 79:5319-20, 1957. 34) Cavalieri, L. F. and Rosenberg, B. The interpretation of bacterial transformation data. Proc. Nat. Acad. Sci. U.S. 44:853-7, 1958. 35) Chakrabarti, S. G. and Litwack, G. Apparent transfer of lysozyme resistance by DNA preparations. Bacterial. Proc. 60:75, 1960. 36) 37) 38) 39) 40) 41) 42) 43) 44) 45) 46) 47) 48) 49) -4- CIBA Foundation Symposium. Drug Resistance in Micro-organisms: Mechanisms of Development. Boston: Little, Brown, 1957. Corey, R. R. and Starr, M. P. Genetic transformation of colony type in Xantomenas phaseoli. J. Bacterial. 74:141-5, 1957. Corey, R. R. and Starr, M. P. Genetic transformation of streptomycin resistance in Xanthomonas phaseoli. J- Bacterial. 74:146-50, 1957. DeFillipes, F. M. and Guild, W. R. Irradiation of solutions of transforming DNA. Radiation Research 11:38-53, 1959. Demerec, M., et. al. Bacterial genetics. Carnegie Inst. Wash. Yearbook 56:368-76, 1956-57. Demerec, M., et. al. Bacterial genetics. Carnegie Inst. Wash. Yearbook 57:390-406, 1957-58. Doty, P., Marmur, J. and Sueoka, N. The heterogeneity in properties and functioning of deoxyribonucleic acids. Brook- haven Symposia in Biol. 12:1-16, 1959. Doty, P., Marmur, J. Eigner, J. and Schildkraut, C. Strand separation and specific recombination in deoxyribonucleic acids: physical chemical studies. Proc. Nat. Acad. Sci. 46: 461-76, 1960. Dove, W. F. and Yamane, T. The complete retention of transforming activity after reversal of the interaction of DNA with mercuric ion. Biochem. Biophys. Res. Communs. 3:608-12, 1960. Drew, R. M. y-irradiation of pneumoaoccus deoxyribonucleic acid. Radiation Research 3:116-20, 1955. Drew, R. M. Induction of resistance to A-methopterin in Diplococcus pneumoniae by deoxyribonucleic acid. Nature 179: 1251-2, 1957. Ellison, 0. A. and Beiser, S. A. The ultraviolet inactivation of pneumococcus transforming factor. Radiation Research 12:381-8, 1960. Ephrati-Elizur, E. and Zamenhof, S. Incorporation of 5- bromouracil into transforming principle of Bacillus subtilis and its biological effects. Nature 184:472-3, 1959. Ephrati-Elieur, E., Srinivasan, P. R. and Zamenhof, S. Genetic analysis by means of transformation of histidine linkage groups in Bacillus subtilis. Proc. Nat. Acad. Sci. U.S. 47: 56-63, 1961. -5- 50) 51) 52) 53) 54) 55) 56) 57) 58) 59) 60) 61) 62) Ephrussi-Taylor. Genetic function and molecular structure of DNA. Proc. Intern. Congr. Biochem., 3rd Congr., Brussels, 1955. N-Y.2 Academic, 1956. Pp. 333-9. Ephrussi-Taylor, H. and Lafar et, R. Inactivation, par les rayons X, d'un facteur transformant du pneumocoque. Biochim. et Biophgs. Acta 16:183-97, 1955. Ephrussi-Taylor, Harriett. X-ray inactivation studies on solutions of transforming DNA of pneumococcus, in S.ymosium on the Chemical Basis of Heredity, ed. by W. D. McElroy and Bentley Glass. Baltimore: John Hopkins Press, 1957. Pp 299-320. Ephrussi-Taylor, H. State of transforming deoxyribonucleic acid (DNA) during the first phase of bacterial transformation. Compt. rend. SOC. biol. 154:1951-5, 1960. Fox, M. S. and Hotchkiss, R. D. Phenotypic expression of a selective genetic character introduced by transformation. Congr. intern. biochim,, Re'sume's Communs., 3rd Congr. Brussels, 1955. P. 74. Fox, M. S. Deoxyribonucleic acid (DNA) incorporation by transformed bacteria. Biochem. et Biophys. Acta 26:83-5, 1957. Fox, M. S. and Hotchkiss, R. D. Initiation of bacterial trans- formation. Nature 179:1322-5, 1957. Fox, M. S. and Hotchkiss, R. D. Fate of transforming deoxy- ribonucleate following fixation by transformable bacteria. I. Nature 187:1002-3, 1960. Fox, M. S. Fate of transforming deoxyribonucleate following fixation by transformable bacteria. II. Nature 187:1004-6, 1960. Fuerst, C. R., Jacob, F., and Wollman, E. L. D&termination de liaisons gknQtiques, chez Escherichia coli K-12, a l'aide de radiophosphore. Compt rend. 243:2162-4, 1956. Fukuda, S. and Tsuzuki, K. Interspecific induction of transformation of bacteria with respect to phage-susceptibility. Nature 190:937-8, 1961. Ginoza, W. and Guild, W. R. On the inactivation of transforming DNA by temperatures below the melting point. Proc. Nat. Acad. Sci. U.S. 47:633-9, 1961. Ginoza, W. and Zimm, B. H. Mechanisms of inactivation of deoxyribonucleic acids by heat. 47:639-52, 1961. Proc. Nat. Acad. Sci. w 63) 64) 65) 66) 67) 68) 69) 70) 71) 72) 73) 74) 75) -6- Goodgal, S. H. and Herriott, R. M. Uptake of transforming DNA and its penetration into Hemophilus influenzae. Federation Proc. 15:590A, 1956. Goodgal, S. H. and Herriott, R. M. Studies on transformation of Hemophilus influenzae, in Symposium on the Chemical Basis of Heredity, ed. by W. D. McElroy and Bentley Glass. Baltimore: John Hopkins Press, 1957. Pp. 336-340. Goodgal, S. H., Rubert, C. S. and Herriott, R. M. Photo- reactivation of Hemophilus influenzae transforming factor for streptomycin resistance by an extract of Escherichia coli B. Discussion, in Symposium on the Chemical Basis of Heredity, ed. by W. D. McElroy and Bentley Glass. Baltimore: John Hopkins Press, 1957. Pp. 341-50. Goodgal, S. H. and Herriott, R. M. A study of linked trans- formations in Hemophilus influenzae. Records Genet. Sac. Am. 26:372-3, 1957. - Goodgal, S. H., Rupert, C. S., and Herriott, R. M. Molecular weight and in vitro photorestoration studies of a trans- forming factor for Hemophilus influenxae. Trans. Faraday & 53:257d, 1957. Goodgal, S. H. Expression and segregation in Hemophilus influenzae transformations. Proc. Intern. Congr. Genet., 10th Congr., Montreal, 1958. 1I:lOO. Goodgal, S. H. and Melechen, N. E. Synthesis of transforming DNA in the presence of chloramphenicol. Biochem. Biophys. Research Communs. 3:114-8, 1960. Green, D. MacDonald. A host-specific variation affecting relative frequency of transformation of two markers in pneumococcus. Exp. Cell Research 18:466-80, 1959. Greer, S., Heldemuth, L. and Zamenhof, S. Transformation to auxotrophy in B. subtilis. Federation Proc. 20:255, 1961. Guild, W. R. and DeFillppes, F. M. Ionizing radiation and ultrasonic evidence for a minimum unit of transforming principle DNA. Biochim. et Biophys. Acta 26:241-51, 1957. Guild, W. R. Physical studies of transforming principle. Proc. 1st Natl.Biophys. Conf. New Haven: Yale University Press, 1959. P. 228. Guild, W. R. Radiation sensitivity of transforming DNA with and without oxygen. Radiation Research 11:444-5, 1959. Hall, R. H. and Gale, G. 0. New observations on pneumococcal transformations in viva. Proc. Sac. Exp. Biol. Med. 101: 487-91, 1959. -7- 75) 76) 77) 78) 79) 80) 81) 82) 83) 84) 85) 86) Hall, R. H. and Gale, G. 0. New observations on pneumococcal transformations in viva. Proc. Sac. Exp. Biol. Med. 101: 487-91, 1959. *Hashimoto, K. The transformation of streptomycin resistance in pneumococcus. I. Transformation of streptomycin indif- ference and resistance. Nippon Saikingaku-Zasshi (Japan. J. Bacteriol.1 10:933-8, 1955. & 51:1061b, 1957. *Hashimoto, K. II. Transformation of the streptomycin dependence. Nippon Saikingaku Zasshi (Japan. J. Bacterial.) 10:1049-53, 1955. CA 51:10651f, 1957. - Hashimoto, K. Further studies on the transformation of streptomycin resistance in pneumococci. Japan'J. Microbial. l:l-9, 1957. Herriott, R. M. Formation of heterozygotea by annealing a mixture of transforming DNAs. Proc. Nat. Acad. Sci. U.S. 47: 146-53, 1961. Hotchkiss, R. D. The biological role of the deoxypentose nucleic acids, in The Nucleic Acids, ed. by E. Chargaff and J. N. Davidson. New York: Academic, 1955. 11:443-453. Hotchkiss, Il. D. Bacterial transformation. J. Cellular Comp. Physiol. 45:1-22, 1955. Hotchkiss, R. D. The genetic organization of the deoxyribo- nucleate units functioning in bacterial transformation, in Enzymes: Units of Biological Structure and Function, ed. by 0. H. Gaebler, New Yorkr Academic, 1956. Pp. 119-30. Hotchkiss, R. D. Criteria for quantitative genetic transfor- mation of bacteria, in Symposium on the Chemical Basis of Heredity; ed. by W. D. McElroy and Bentley Glass. Baltimore: John Hopkins Press, 1957. Pp. 321-335. Hotchkiss, R. D. Size limitations governing the incorporation of genetic material in the bacterial transformations and other nonreciprocal recombinations. Symposia Sot. Exp. Biol. 12:49-59, 1958. Hotchkiss, R. D. and Evans, A. H. Analysis of the complex sulfonamide resistance locus of pneumococcus. Cold Spring Harbor Symposia &ant. Biol. 23:85-97, 1958. Hutchinson, P. and Arena, J. Destruction of the activity of deoxyribonucleic acid in irradiated cells. Radiation Research 13:137-47, 1960. -a- 87) Imshenetskii, A. A., Perova, K. Z., Zaitseva, T. A. and Belozerskil ,9 A. N. Transformation of streptom.ycin resistance~.in staphylococci by means of DNA. -Microbiology 28:175-a, 1960. (Tr. from Mikrobiologiya 28:187-90, 1959.) 88) Imshenetskii, A. A. and Perova, K. Z. Transformation carried out by cell-free extracts. (Tr. Microbiology 28:371-374, 1961. from Mikrobiologiya 29:505-11, 1960.) 89) Imshenetskii, A. A. and Perova, K. Z. Transformation in microbes induced by filtrates passed through bacterial filters. Microbiology 29:486-9, 1961. (Tr. from Mikrobiologiya 29:673-8, 1960.) 90) *Jablofiski, L. and Poplawski, S, Deoxyribonucleic acid in Brucella. Preliminary study of transformation. Acta Microbial Polon. 101147-155, 1961. 91) Jackson, S., Macleod, C. M. and Krauss, M. R. Determination of type in capsulated transformants on penumococcus by the genome of non-capsulated donor and recipient strains. J. Exp. Med. 109:429-38, 1959. 92) *Jarai, M. Transformation in streptomyces. Acta Microbial. Acad. Sci. Hung. 8:81-7, 1961. 93) Hogness, D. S. and Kaiser, A. D. The transformation of Escherichia coli with deoxyribonucleic acid isolated from bacteriophage hdg. J. Mol. Biol. 2:392-415, 1960. 94) *Kimura, Y. The streptomycin resistance induced by deoxy- rinonucleic acid from streptomycin-resistant bacteria. II. The induced streptomycin resistance and the R+S variation. Nippon Saikingaku Zasshi (Japan.J. Bacteriol.1 11:199-204, 1956. CA 51:18013b, 1957. - 95) *Kinoshita, K. The streptomycin resistance induced by the deoxyribonucleic acid from streptomycin-resistant bacteria. Nippon Saikingaku Zasshi 10:463-a, 1955. CA 51:6764e, 1957. - 96) Kohiyama, M. and Saito, H. Stimulation of genetic transfor- mation in Bacillus subtilis by biological inactive DNA and polyphosphate. Biochim. 1960. et Biophys. Acta 41:180-l, 97) Kohiyama, M. Genetic research on Bacillus subtilis. rend. sot. biol. 154:690-2, 1960. Compt. 98) Kohiyama, M., Saito, H. and Ikeda, Y. An hypothesis on genetic transformation in Bacillus subtilis. Compt. rend. sot. biol. 154:1893-4, 1960. -9- ,,I 99) *Kohoutov&, M. The effect of monovalent cations on the trn,nsformxti on of type specificity in pneumococci. Cesko- ~k~3;:44-53, 1958. 77 ______ - --_..- -- .~. slov. Mikrobiol BA 32:4111~, 1720. - 100) Kohoutova, M. Effect of univalent cations on type specifi- city transformation in pneumococci. Nature 182:188-9, 1958. 101) *Kohoutova, M. The sodium and potassium concentration in rabbit anti-R pneumococcal sera and its relationship to type-specific transformation. Folia Mikrobiol. 4:130-2, 1959. BA 35:13824; 1960. - 102) Kohoutov&, M. Mechanism of the transformation of poly- saccharide capsule in pneumococcus. Nature 190:1171-3, 1961. 103) Lacks, S. and Hotchkiss, R. D. Genetic material determining and enzyme activity in pneumococcus. Biochim. et Bioph.ys. Acta 39:508-18, 1960. 104) Latarjet, R. Effects of radiation and peroxides on viral and bacterial functions linked to DNA specificity, in Ciba Foundation Symposium on Ionizing Radiation and Cell Metabolism. Boston: Little, Brown, 1956. Pp. 275-99. 105) Latarjet, R. Photorestauration, katalase-restauration and "pox"-effect bei bakterien und bakteriophagen. Strahlen- therapie 101:580-98, 1956. 106) *Latarjet, R. and Cherrier, N. Intern. Congr. Photobiol., 2nd Congr., Turin, 1957. P. 131. 107) Latarjet, R., Rebeyrotte, N. and Demerseman, P. Action of organic peroxides on a transforming factor of pneumococcus, in Organic Peroxides in Radiobiology, ed. b,y M. Haissinskv, New York: Pergamon, 1958. Pp. 61-72. 108) Latarjet, R., Rebeyrotte, N. and Demerseman, P. Action de peroxydes organigues sur un facteur transformant du pneumocogue. Actions chim. et biol. radiations 4:61-72, 1958. 109) Latarjet, R. Radiobiological studies on transforming nucleic acids. Strahlentherapie 110:443-50, 1959. 110) Latarjet, R., Ephrussi-Taylor, H. and Rebeyrotte, N. On the target size of a transforming factor based on x-ray inactivation. Radiation Research Suppl. 1:417-30, 1959. 111) Leidy, G., Hahn, E. and Alexander, H. E. On the specificity of the desoxyribonucleic acid which induces streptomycin resistance in Hemophilus. J. Exp. Med. 104:305-20, 1956. - 10 - il2) Leidy, G., Hahn, E. and Alexander, H. E. Interspecific transformation in Hemophilus; a possible index of relationship between H. influenzae and H. aegyptius. Proc. Sot. Exp. Biol. Med. 102:86-S, 1959. 113) *Lejeune, J. The structure of hereditary substance. Rev. M&d. Liege 13:533-41, 1958. 114) Lerman, L. S. Chromatographic fractionation of the transforming principle of the pneumococcus. Biochim. et Biophys. Acta 18:132-4, 1955. 115) Lerman, L. S. and Tolmach, L. J. Kinetic studies of genetic transformation in pneumococcus. Proc. 1st. Natl. Biophys. Conf., 1957. New Haven: Yale University Press, 1959. P. 436. 116) Lerman, L. S. and Tolmach, L. J. Genetic transformation. I. Cellular incorporation of deoxyribonucleic acid (DNA) accompanying transformation in pneumococcus. Biochim. et Biophys. Acta 26:68-82, 1957. 117) Lerman, L. S. Estimation of the critical DNA segment required for genetic transformation. Intern. Congr. Genet. Proc. 10th Congr., Montreal, 1958 2:164. 118) Lerman, L. S. and Tolmach, L. S. Genetic transformation. II. The significance of damage to the DNA molecule. Biochim. et Biophys. Acta 33:371-87, 1959. 119) *Lindegren, C. C. The biological function of deoxyribonucleic acid. J. Theoretical Biol. 1:107-19, 1961. BA 36:54675, 1961. - 120) Litman, R. M. and Ephrussi-Taylor, H. 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Proc. 61:90, 1961. 133) Nester, E. W. and Lederberg, J. Linkage of genetic units of Bacillus subtilis in deoxyribonucleic acid (DNA) trans- formation. Proc. Nat. Acad. Sci. U.S. 47:52-5, 1961. 134) *Odaka, T. Nippon Saikingaku Zasshi (Japan. J. Bacterial.) 14: 254, 1959. 135) KOdaka, T. Acquisition of streptomycin resistance in pneumococci by means of transformation reaction. II. Relationship between S-R variation and the transformability. Nippon Saikingaku Zasshi (Japan. J. Bacterial.) 14:266-70, 1959. BA 35:4835, 1960. - 136) *Odaka, T. Acquisition of streptomycin resistance in pneumococci by means of transformation reaction. III. Transformation of dialyzed media and effect of fatty acids. Nippon Saikingaku Zasshi (Japan. J. Bacterial.) 14:796-800, 1959. BA 35135204, 1960. - 137) *Odaka, T. Acquisition of streptomycin resistance in pneumococci by means of transformation reaction. IV. Transformation in partially defined media containing no serum factor, Nippon Saikingaku Zasshi (Japan. J. Bacterial.) 14:965-8, 1959. BA 35r35204, 1960. - - 12 - 138) Odaka, T. and Watanabe, T. Transformation reaction of pneumococci in the absence of serum and the effect of fatty acids. Japan J. Exp. Med. 29:407-16, 1959. 139) Odaka, T. and Watanabe, T. Transformation reaction of pneumococci in the absence of serum factor. Nature 184: 471-2, 1959. 140) *Opara, 2. Selected problems from genetics of bacteria. Med. Doswiadczalna i. Mikrobiol. 11:235-52, 1957. BA 33:46061, 1959. - 141) *Opara, 2. Effect of chloromycin on the transformation of streptomycin resistance in pneumococci. Bull. acad. polon. sci., Ser. sci. biol. 6r471, 1958. CA 53:19025f, 1959. - 142) *Opara, 2. Transformation co-factors of pneumo'coccus R36A. Polska Acad. Nauk, Zeszyty Problemowe Nauki Polskiej 14:41- 9, 1958. CA 53:5386d, 1959. - 143) *Opara-Kubinska, 2. Investigations on bacterial transformations. I. 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