William A. Charini1 and Norman L. Letvin2
1,2Harvard Medical School, Beth Israel Deaconess Medical Center, Boston, MA 02158, USA
Macaque species are proving to be crucially important animals for studying AIDS pathogenesis and exploring HIV vaccine strategies. In both of these experimental areas it is important to measure virus-specific CTL responses with precision. This can best be done with knowledge of epitopes and their restricting MHC class I alleles. We now begin categorizing these epitope-specific CTL responses. We only include those epitopes that have been published and those in which the MHC class I alleles that bind these peptides have been characterized. To date, this has only been done in Macaca mulatta(rhesus monkeys), although M. nemestrina (pig-tailed macaques) and M. fascicularis (crab-eating macaques) are frequently used in AIDS experimental work. Both the nucleotide and predicted amino acid sequences of the MHC class I alleles are shown. With the expanding use of SHIV constructs for these studies, we include CTL epitopes of both SIV and HIV-1 envelopes. We hope to maintain an up-to-date listing by adding newly defined epitopes as they become available. Please contact us with contributions at:
Bette Korber William A. Charini Norman L. Letvin phone: 505-665-4453 617-667-0250 617-667-2766 fax: 505-665-3493 617-667-8210 617-667-8210 email: btk@t10.lanl.gov wcharini@bidmc.harvard.edu nletvin@bidmc.harvard.edu
Viral protein Epitope Restricting MHC GenBank Acc. No. Reference class I alleler SIVmac Gag CTPYDINQM Mamu-A*01 U50836 Miller, et al. 1991 Allen et al., 1998 in press SIVmac Env YNLTMKCR Mamu-A*02 U50837 Watanabe, et al. 1994 EITPIGLAP Mamu-B*01 U42837 Yasutomi, et al. 1995 HIV-1 Env KPCVKLTP Mamu-B*12 Voss, et al. 1996 NNLLRAIEA Mamu-A*08 Voss, et al. 1996
Alignment of Sequences of Restricting Alleles
The nucleotide sequences of cDNA clones of the five MHC class I
restricting alleles of rhesus monkey ( Macaca mulatta) described in Table~1
are shown aligned below with their predicted amino acid sequences.
CONSENSUS CTCCTCCTGgTGCTCTC?GGGgCcCTGgcCCTGACccAGACCtGGGCcGGCTCgCACTCCaTGAgGTATTTCtaC 74 Mamu-A*01 -----------------A----------T-------------C----G-----T----------A---------- 75 Mamu-A*02 -----------------A-----------------T------C----A-----T--------------------- 75 Mamu-A*08 -----------------C-----G------------------------------------T-------------- 75 Mamu-B*01 ---------C-------G------------------------------------------------------C-- 75 Mamu-B*12 ---------C-------G---A-----T--------G-----------------------------------AG- 75 -> leader peptide -> alpha 1 domain CONSENSUS _L__L__L__v__L__S__G__a__L__a__L__T__q__T__w__A__G__S__H__S__m__r__Y__F__y_ 25 Mamu-A*01 ----------------------------V--------------R--------------------K---------- 25 Mamu-A*02 -------------------------------------------R------------------------------- 25 Mamu-A*08 -------------------------------------------------------------L------------- 25 Mamu-B*01 ----------L--------------------------------------------------------------H- 25 Mamu-B*12 ----------L-----------T-----S--------E-----------------------------------S- 25 CONSENSUS ACc?CcgTGTCCCGGCCCGGCCGCgGGgAgCCCcGcTtcaTCgccGTgGGCTACGTGGACGACACGCAGTTCGTG 148 Mamu-A*01 ---T--A--------------------C----------------------------------------------- 150 Mamu-A*02 ---T--A-----------------T-------------------------------------------------- 150 Mamu-A*08 ---G-----------------------C--------------T-------------------------------- 150 Mamu-B*01 --GC--------------------------------------T-------------------------------- 150 Mamu-B*12 ---G-A------------------C----A---T-G-ATC---AA--C--------------------------- 150 CONSENSUS _T__?__v__S__R__P__G__R__g__e__P__r__f__i__?__V__G__Y__V__D__D__T__Q__F__V_ 48 Mamu-A*01 ----S--M--------------------Q--------------A------------------------------- 50 Mamu-A*02 ----S--M-----------------W-----------------A------------------------------- 50 Mamu-A*08 ----A-----------------------Q--------------S------------------------------- 50 Mamu-B*01 ----P--------------------------------------S------------------------------- 50 Mamu-B*12 ----A--------------------R--------W--Y--L--E------------------------------- 50 CONSENSUS CGgTTCGACAGCGACGCCGaGAGtC?gAGgatGGAGCCGCGGGCGCCgTGGgTgGAGCAGGAGGGgCCgGAGTAT 222 Mamu-A*01 -------------------C---C-AA--------------------------------------T--------- 225 Mamu-A*02 -------------------C---C-A---------------------------------------T--A------ 225 Mamu-A*08 -------------------------C---AGA------------------------------------------- 225 Mamu-B*01 --A----------------------G---------------------C---A-A--------------------- 225 Mamu-B*12 -------------------------C------------------------------------------------- 225 CONSENSUS _R__F__D__S__D__A__e__S__?__R__m__E__P__R__A__P__W__v__E__Q__E__G__P__E__Y_ 72 Mamu-A*01 -------------------A-----Q------------------------------------------------- 75 Mamu-A*02 -------------------A-----Q------------------------------------------------- 75 Mamu-A*08 -------------------------P-----E------------------------------------------- 75 Mamu-B*01 -------------------------R--------------------------I---------------------- 75 Mamu-B*12 -------------------------P------------------------------------------------- 75 CONSENSUS TGGGAccgGgAgACACGGAac??cAAGgcCgagaCACAGAatgaCCgAGtGaaCCTGcgGAaCctGCgC?GCTAC 294 Mamu-A*01 ---------------------ATG---A---------------C--C--------------C-----T-C----- 300 Mamu-A*02 ---------------------ATG-------------------C--C----------------------G----- 300 Mamu-A*08 ---------A-C-------T-TA--------C---------CT------A-GG-----A----------G----- 300 Mamu-B*01 --------------------GGC-----G-A-CG------C--------A-----------T-GC--T-A----- 300 Mamu-B*12 -----AGA-C-----------GC-------A-CG------C-----------G----G-----------C----- 300 CONSENSUS _W__d__r__e__T__R__n__?__K__a__?__t__Q__n__?__r__v__n__L__r__n__l__r__?__Y_ 93 Mamu-A*01 ----------------------M-----T--E-----------A--P--------------T-----L--R---- 100 Mamu-A*02 ----------------------M--------E-----------A--P-----------------------G---- 100 Mamu-A*08 ----------N--------I--Y--------A-----------Y-----E--G-----Q-----------G---- 100 Mamu-B*01 -------------------K--A-----G--N--A-----T--D-----E-----------I--A--L--S---- 100 Mamu-B*12 ----E--E--Q-----------A--------N--A-----T--D--------S-----G-----------R---- 100 CONSENSUS TACAACCAGAGCGAGgccGGGTCtCACACc?tCCAGA?gATGtacGGCTGCGACCTGGGgCCcGACGGGCGCCTC 367 Mamu-A*01 ------------------------------C------G----GTT------------------------------ 375 Mamu-A*02 ------------------------------A------G------------------------G------------ 375 Mamu-A*08 -----------------------C------TA-----C------------------------A------------ 375 Mamu-B*01 ---------------A-G------------C------T----C----------------A--------------- 375 Mamu-B*12 ----------------GG-----------TG------TA--------------------A--------------- 375 -> alpha 2 domain CONSENSUS _Y__N__Q__S__E__a__G__S__H__T__?__Q__?__M__y__G__C__D__L__G__P__D__G__R__L_ 116 Mamu-A*01 -------------------------------L-----R-----V------------------------------- 125 Mamu-A*02 -------------------------------I-----R------------------------------------- 125 Mamu-A*08 -------------------------------Y-----T------------------------------------- 125 Mamu-B*01 ----------------T--------------L-----M-----H------------------------------- 125 Mamu-B*12 ----------------G--------------V-----I------------------------------------- 125 CONSENSUS CTCCGCGGGTAT?AcCAGt?CGCCTACGACGGCAaGGATTACATCGCCCTGAACGAGGACCTGCgCTCCTGGACc 440 Mamu-A*01 ------------G-A----A------------------------------------------------------- 450 Mamu-A*02 ------------C------C------------------------------------------------------- 450 Mamu-A*08 ------------G------C--------------G---------------------------------------G 450 Mamu-B*01 ------------T-----CG--------------------------------------------A---------- 450 Mamu-B*12 ------------C------T------------------------------------------------------- 450 CONSENSUS _L__R__G__Y__?__Q__?__A__Y__D__G__k__D__Y__I__A__L__N__E__D__L__r__S__W__T_ 139 Mamu-A*01 -------------E-----Y------------------------------------------------------- 150 Mamu-A*02 -------------H-----S------------------------------------------------------- 150 Mamu-A*08 -------------D-----S--------------R---------------------------------------- 150 Mamu-B*01 -------------Y-----R--------------------------------------------H---------- 150 Mamu-B*12 -------------H-----F------------------------------------------------------- 150 CONSENSUS GCcGCGGACaTGGCgGCTCAGAACACCCAGCGcAAGTGGGAGGcGGcgggTgagGCgGAGcgg?tcAGAgCCTAc 514 Mamu-A*01 --G------G---------------------------------------A--T-------A-CA-G--------- 525 Mamu-A*02 -------------------------------------------------------------A-CA----A----- 525 Mamu-A*08 --A-----------T------------------------------------------------T-----A----- 525 Mamu-B*01 ---------T----------------------G--------------C----T---A----A-CGG--------- 525 Mamu-B*12 -------------------------------------------G--ACC--T-T---------T----------T 525 CONSENSUS _A__A__D__m__A__A__Q__N__T__Q__R__K__W__E__a__a__g__?__A__E__?__?__R__a__Y_ 161 Mamu-A*01 ----------V--------------------------------------D--V--------S--M---------- 175 Mamu-A*02 ----------------------------------------------------E--------Q--H-----T---- 175 Mamu-A*08 ----------------------------------------------------E--------R--F-----T---- 175 Mamu-B*01 ----------L-----------------------------------------V--------Q--R---------- 175 Mamu-B*12 -------------------------------------------G--D--R--Y--------R--F---------- 175 CONSENSUS CTGGAGGGCcaGTGCgTGGAGTGGCTCCgCAGATAcCTGGAGAAcGGGAAGGAGACgCTGCAGCGCgCGGAcCCC 589 Mamu-A*01 ----------------------------C---------------G---------------------A-------- 600 Mamu-A*02 ---------G-----C----------------------------------------------------------- 600 Mamu-A*08 ---------G-------------------------T--------------------------------------- 600 Mamu-B*01 ----------G---------------------------------G-----------A------------------ 600 Mamu-B*12 ----------G------------------------------------------------------------T--- 600 alpha 3 domain -> CONSENSUS _L__E__G__?__C__v__E__W__L__r__R__Y__L__E__n__G__K__E__T__L__Q__R__a__D__P_ 185 Mamu-A*01 ----------Q-----------------P--------------K-----------------------T------- 200 Mamu-A*02 ----------E-----L---------------------------------------------------------- 200 Mamu-A*08 ----------E---------------------------------------------------------------- 200 Mamu-B*01 ----------R--------------------------------K------------------------------- 200 Mamu-B*12 ----------R---------------------------------------------------------------- 200 CONSENSUS CCcAAGACACAcGTGACCCACCACCCcGTCTCTgACCAtGAGgCCAcCCTGAGGTGCTGGGCCCTGGGCTTCTAC 664 Mamu-A*01 -----------T--------------------------------------------------------------- 675 Mamu-A*02 -----------T--------------------------A------------------------------------ 675 Mamu-A*08 --------------------------------------------------------------------------- 675 Mamu-B*01 --A-----------------------T---------------A---T---------------------------- 675 Mamu-B*12 --A------------------------------A----------------------------------------- 675 CONSENSUS _P__K__T__H__V__T__H__H__P__V__S__d__h__E__a__t__L__R__C__W__A__L__G__F__Y_ 210 Mamu-A*01 --------------------------------------------------------------------------- 225 Mamu-A*02 -------------------------------------Q------------------------------------- 225 Mamu-A*08 --------------------------------------------------------------------------- 225 Mamu-B*01 -------------------------------------------T--I---------------------------- 225 Mamu-B*12 ----------------------------------N---------------------------------------- 225 CONSENSUS CCTGcGGAGATCACACTGACCTGGCAGCGGGATGGgGAGGACCAAACTCAGGACACgGAGCTcGTGGAGACCAGG 739 Mamu-A*01 --------------------------------------------------------------------------- 750 Mamu-A*02 -----------------------------------A--------------------------------------- 750 Mamu-A*08 --------------------------------------------------------------------------- 750 Mamu-B*01 --------------------------------------------------------T-----T------------ 750 Mamu-B*12 ----T---------------------------------------------------C------------------ 750 CONSENSUS _P__a__E__I__T__L__T__W__Q__R__D__G__E__D__Q__T__Q__D__T__E__L__V__E__T__R_ 235 Mamu-A*01 --------------------------------------------------------------------------- 250 Mamu-A*02 --------------------------------------------------------------------------- 250 Mamu-A*08 --------------------------------------------------------------------------- 250 Mamu-B*01 --------------------------------------------------------------------------- 250 Mamu-B*12 ----V---------------------------------------------------------------------- 250 CONSENSUS CCtGcAGGaGAtGGAACCTTCCAGAAgTGGGcgGCTGTGGTGGTGCCTTCTGGAgAgGAGCAGAGATACACcTGt 814 Mamu-A*01 --------------------------------------------------------------------------- 825 Mamu-A*02 --------G--C------------------------------------------A-------------------- 825 Mamu-A*08 ------------------------------------------------------A-------------------- 825 Mamu-B*01 --A-G---------------------A----GA-----------------------A--------------G--C 825 Mamu-B*12 --A-G--------------------------GA-----------------------A--------------G--C 825 CONSENSUS _P__a__G__D__G__T__F__Q__K__W__a__A__V__V__V__P__S__G__e__E__Q__R__Y__T__C_ 260 Mamu-A*01 --------------------------------------------------------------------------- 275 Mamu-A*02 -------------------------------------------------------K------------------- 275 Mamu-A*08 -------------------------------------------------------K------------------- 275 Mamu-B*01 ----G--------------------------G------------------------------------------- 275 Mamu-B*12 ----G--------------------------G------------------------------------------- 275 CONSENSUS CATGTGCAGCATGAGGGtCTGCc?gAGCCCCtCACCCTGAgATGGGAGCCgTcTTCCCAGTCCACCATCCcCATc 888 Mamu-A*01 -----------------------CA------A--------A-----------T---------------------G 900 Mamu-A*02 ----------------------GT----------------------------------------------T---- 900 Mamu-A*08 -----------------------C--------------------------------------------------G 900 Mamu-B*01 -----------------G-----G--------------------------A------------------------ 900 Mamu-B*12 -----------------G-----A--------------------------A------------------------ 900 -> transmembrane domain CONSENSUS _H__V__Q__H__E__G__L__p__e__P__l__T__L__r__W__E__P__s__S__Q__S__T__I__p__i_ 285 Mamu-A*01 -------------------------K-----H--------K-----------F--------------------M- 300 Mamu-A*02 ----------------------R-----------------------------------------------L---- 300 Mamu-A*08 -------------------------------------------------------------------------M- 300 Mamu-B*01 --------------------------------------------------------------------------- 300 Mamu-B*12 --------------------------------------------------------------------------- 300 CONSENSUS GTGGGCATCaTTGCTGGCCTGGtTctCCTtGgAgtTGTGGTCActGGAGCTGTGgTcGCTGCTGTGATgTGGAGG 963 Mamu-A*01 ----------------------------------C---------------------T------------------ 975 Mamu-A*02 ---------------------------------A---------T----------A-T-----------A------ 975 Mamu-A*08 ----------------------------------C---------------------------------------- 975 Mamu-B*01 ---------G------------C-G----A-C------------C------------------------------ 975 Mamu-B*12 ---------G------------C-GG---A-C------------C------------------------------ 975 CONSENSUS _V__G__I__i__A__G__L__v__l__L__g__?__V__V__t__G__A__V__v__A__A__V__m__W__R_ 309 Mamu-A*01 ----------------------------------A---------------------------------------- 325 Mamu-A*02 ----------------------------------I--------I-----------I-----------I------- 325 Mamu-A*08 ----------------------------------A---------------------------------------- 325 Mamu-B*01 ----------V-----------A--V-----A--V---------------------------------------- 325 Mamu-B*12 ----------V-----------A--G-----A--V---------------------------------------- 325 CONSENSUS AGGAAGAGCTCAGaTaGAAAAGGAGGGAGCTACTCTCAGGCTGCaagCAgtGACAGTGCCCAGGGCTCTGATGTG 1038 Mamu-A*01 --------------------------------------------------------------------------- 1050 Mamu-A*02 --------------------------------------------------------------------------- 1050 Mamu-A*08 --------------------------------------------------------------------------- 1050 Mamu-B*01 -------------G-G----------------------------GTC---C------------------------ 1050 Mamu-B*12 -------------G-G----------------------------GTC--AC------------------------ 1050 -> cytoplasmic domain CONSENSUS _R__K__S__S__d__r__K__G__G__S__Y__S__Q__A__A__S__s__D__S__A__Q__G__S__D__V_ 334 Mamu-A*01 --------------------------------------------------------------------------- 350 Mamu-A*02 --------------------------------------------------------------------------- 350 Mamu-A*08 --------------------------------------------------------------------------- 350 Mamu-B*01 -------------G--G---------------------------------------------------------- 350 Mamu-B*12 -------------G--G--------------------------------N------------------------- 350 CONSENSUS TCTCTCACGGCTTgtAAAGTGTGA 1062 Mamu-A*01 ------------------------ 1074 Mamu-A*02 ------------------------ 1074 Mamu-A*08 ------------------------ 1074 Mamu-B*01 -------------AA 1065 Mamu-B*12 --------------A 1065 CONSENSUS _S__L__T__A__c__K__V__$_ 341 Mamu-A*01 ------------------------ 357 Mamu-A*02 ------------------------ 357 Mamu-A*08 ------------------------ 357 Mamu-B*01 -------------$- 354 Mamu-B*12 -------------$- 354References