Proteogenomics, and Other Non-Traditional Applications of Mass Spectrometry |
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| Launch in standalone player | |
| Air date: | Friday, January 09, 2009, 10:00:00 AM |
| Category: | Proteomics |
| Runtime: | 75 minutes |
| NLM Title: | Proteogenomics, etc. [electronic resource] / Vineet Bafna. |
| Author: | Bafna, Vineet. National Institutes of Health (U.S.) |
| Publisher: | [Bethesda, Md. : National Institutes of Health, 2009] |
| Abstract: | (CIT): In this talk, we will discuss some novel uses of mass spectrometric data. First, we apply MS2 data for eukaryotic gene discovery. To do this, we must overcome a number of challenges, including computational bottlenecks, confidence assignment to peptide identification, and identification of spliced-peptides, (peptides that cross splice junctions). Our proteogenomic survey of Arabidopsis resulted in the identification of over 800 novel genes, which will be included in TAIR 9 release. Second, we apply mass spectrometry data for antibody sequencing using a novel technique, 'genome-anchoring'. Our method allows the unambiguous reconstruction of mouse antibody sequence using tandem MS2 data. Finally, we describe a novel computational method for computing the relative abundance of a protein relative to another, contradicting the apparent truism that protein quantification is limited to measuring relative expression of a single protein. |
| Subjects: | Antibodies--genetics Computational Biology Genomics Mass Spectrometry Peptide Mapping Proteomics |
| Publication Types: | Government Publications Lectures |
| NLM Classification: | QU 58.5 |
| NLM ID: | 101496152 |
| CIT File ID: | 14847 |
| CIT Live ID: | 7343 |
| Permanent link: | http://videocast.nih.gov/launch.asp?14847 |