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Primer Information
Each unique primer pair corresponds to one UniSTS ID that is unique and stable over time.
"PCR product size" displays a summary of all size data submitted from data sources.
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Cross References
The Gene connection is established when any of the following conditions are met:
The UniGene connection is made based on e-PCR results on mRNAs and ESTs.
The dbSNP connection is made when SNP is located within the STSs.
RHDB, MGI, RGD and GDB links are made based on data obtained from these external databases.
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Mapping Information
All mapping information available for a given STS is shown.
Publications and data sources for each map are listed in UniSTS home page.
The "Sequence" map indicates an STS map on the sequenced genome that was computed using e-PCR program. The e-PCR program was run with following setting: hash of 12 nt, 3 discontiguous words, 1 mismatch allowed, 1 gap allowed, margin of 200 bp (W=12 F=3 N=1 G=1 M=200). When an STS hit more than 2 locations on mapped contigs or hit more than 5 locations on unmapped contigs, it was considered repetitive and excluded from annotation on Sequence map, and warning was issued in UniSTS under organism section as"Warning! This marker matched more than 2 locations on genome by e-PCR and therefore excluded from annotation."
Clicking the "Map Viewer" button opens a graphical display showing the position of the marker on the chromosome using the Map Viewer.
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Electronic PCR Result
The e-PCR program is run allowing one mismatch, one gap in each primer alignment, and a size difference of +/- 50 bp between expected size and actual size (N = 1, G = 1 M = 50).
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