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SAGE Library Info
| New SAGE Library Name: | SAGE_Prostate_carcinoma_CL_LNCaP-T |
| Old SAGE Library Name: | SAGE_CPDR_LNCaP-T |
| Organism: | Homo sapiens |
| Library Keywords: | prostate, carcinoma, cell line, short SAGE, SAGE, non-normalized |
| #Total tags: | 45,375 |
| #Tags excluding linkers: | 43,542 |
| #Unique tags: | 15,922 |
| Tissue: | prostate |
| Cell/Histology type: | carcinoma |
| Tissue preparation: | cell line |
| Mutations: | null |
| Patient age: | null |
| Sex: | null |
| Other info: | LNCaP cells were maintained in RPMI 1640 supplemented with 10% fetal bovine serum and experiments were performed on cells between passages 20 and 30. For the studies of androgen regulation, charcoal/dextran stripped androgen-free FBS was used. LNCaP cells were cultured first in RPMI 1640 with 10% cFBS for 5 days and then stimulated with an non-metabolizable androgen analog R1881 (DUPONT, Boston, MA) 10-8 M for 24 hours (for generating LNCaP-T library). LNCaP cells identically treated but without R1881 treatment served as control (for generating LNCaP-C library). Cells were harvested at indicated time and polyA+ RNA was double-selected with Fast Track kit (Invitrogene) SAGE libraries were generated according to the procedure provided by Dr. Kinzler (Johns Hopkins University School of Medicine, Baltimore, MD). |
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