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SAGE Library Info
| New SAGE Library Name: | SAGE_Cartilage_dedifferentiated_chondrosarcoma_metastasis_CL_UIGX0 |
| Old SAGE Library Name: | |
| Organism: | Homo sapiens |
| Library Keywords: | cartilage, dedifferentiated, chondrosarcoma, metastasis, cell line, adult, female, short sage |
| #Total tags: | 88,439 |
| #Tags excluding linkers: | 88,439 |
| #Unique tags: | 27,818 |
| Tissue: | cartilage |
| Cell/Histology type: | Dedifferentiated chondrosarcoma lung metastasis |
| Tissue preparation: | cell line |
| Mutations: | N/A |
| Patient age: | 45 years old |
| Sex: | female |
| Other info: | Tissue section of lung left lower lobe, diaphragmatic anterior metastatic lesion from 45 years old female with metastatic dedifferentiated chondrosarcoma were used to obtain mono-dispersed cells. The cell line was established from the cells after culturing in RPMI-1640 supplemented with 10% FBS and 0.1% gentamicin sulfate (Gemini Bioproducts). The cell line had gone through 4 doublings to yield 2 x 107 cells. The cell line was routinely screened for mycoplasma sps. Using a PCR-based ELISA detection assay (Roche). Cell manipulations were always performed on 80-90% confluent cultures for consistency. mRNA was isolated using a direct mRNA extraction kit. |
| Tagging enzyme: | BsmFI |
| Anchoring enzyme: | NlaIII |
| Tissue or cell line supplier: | Hendrix, M. Seftor, E. Dep of Anatomy and Cell Biology, Holden Comprehensive Cancer Center (University of Iowa). |
| Library preparer: | S. Malchenko |
| Prepared in lab of: | B. Soares Laboratory (University of Iowa), contributed to the National Cancer Institute Genome Anatomy Project |
| References: | |
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