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SAGE Library Info
| New SAGE Library Name: | SAGE_Cartilage_chondrosarcoma_grade_2_CL_UIGE0 |
| Old SAGE Library Name: | |
| Organism: | Homo sapiens |
| Library Keywords: | cartilage, chondrosarcoma, grade ii, cell line, adult, male, short sage |
| #Total tags: | 77,757 |
| #Tags excluding linkers: | 77,757 |
| #Unique tags: | 21,384 |
| Tissue: | cartilage |
| Cell/Histology type: | chondrosarcoma grade 2. |
| Tissue preparation: | cell line |
| Mutations: | N/A |
| Patient age: | 28 years old |
| Sex: | male |
| Other info: | Tissue section of nterior medial aspect of the pelvis from 28 years old male with grade 2 recurrent chondrosarcoma was divided, by gross analysis, into a fibrosis-like sample and was used to obtain mono-dispersed cells. The cell line was established from the cells after culturing in growth medium that contains heat inactivated FBS (Life Technologies Gibco) (12%), DMEM, glucose (4.5 gm/l), HEPES buffer (25 mM) solution and gentamicin sulfate (50 ??g/ml) (Gemini Bioproducts). The cell line had gone through 4 doublings to yield 2 x 107 cells. The cell line was routinely screened for mycoplasma sps. Using a PCR-based ELISA detection assay (Roche). Cell manipulations were always performed on 80-90% confluent cultures for consistency. mRNA was isolated using a direct mRNA extraction kit. |
| Tagging enzyme: | BsmFI |
| Anchoring enzyme: | NlaIII |
| Tissue or cell line supplier: | J. Stevens Dep of Orthopedic Surgery, University of Iowa |
| Library preparer: | S. Malchenko |
| Prepared in lab of: | B. Soares Laboratory (University of Iowa), contributed to the National Cancer Institute Genome Anatomy Project |
| References: | |
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