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SAGE

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Digital Karyotyping

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SAGE Library Info

Library ID

New SAGE Library Name:SAGE_Breast_carcinoma_epithelium_PROCR+_AP_IDC29
Old SAGE Library Name:
Organism:Homo sapiens
Library Keywords:human, breast/mammary gland, antibody purified, cancer, invasive ductal carcinoma er+,pr+,her2-, female, adult, extracted short sage

Tag Info

#Total tags:54,164
#Tags excluding linkers:54,080
#Unique tags:19,041

Tissue Info

Tissue:breast
Cell/Histology type:invasive ductal carcinoma ER+,PR+,HER2-
Tissue preparation:antibody purified
Mutations:null
Patient age:55
Sex:female
Other info:cells purified using PROCR antibody, these cells are CD44+ breast "cancer stem" cells

Library Preparation Info

Tagging enzyme:MmeI
Anchoring enzyme:NlaIII
Tissue or cell line supplier:Andrea Richardson, MD.PhD, BWH
Library preparer:Michail Shipitsin, PhD, DFCI
Prepared in lab of:Polyak lab, DFCI
References:Shipitsin et al. Cancer Cell 2007 11: 259-273.


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