shRNA Info
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shRNA Construct
Design rationale
- miR30 flanking sequences of 125 nt on
each end of the hairpin and the miR30 loop increase Drosha and Dicer processing of the expressed hairpin.
- shRNA clones contain 22 sense and 22
anti-sense nucleotide base
pairs. The following criteria were used to select sequences:
- Matched to only exonic (coding) regions of targeted gene
- Contained >3 mismatches to any other gene
- Where possible, shRNAs showed sequence identity between the human and mouse
orthologue of the targeted gene
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