Harlan Animal Colonies Health Evaluation Summary: 09/24/07
Contract N01-AG-7-2113
RATS Barrier 202B
| Animal Facility | Strain | No.Sex | Age | Testing Laboratory/Dates |
|---|
| Location: Indianapolis, IN | F344/NHsd | 2M/1F | 5 months | University of Missouri |
| Barrier/Building: 202B | | 1M/2F | 10 months | MURADIL, Columbia, MO |
| | | | | |
| | | | | Receipt Date: 7/23/07 |
| Ship Date: 7/22/07 | | | | Report Date: 9/14/07 |
Evaluation
| NIA rats submitted for this health monitoring were obtained from NIA-NIH aging colonies maintained in barriers by Harlan. All rats examined were considered to be in a good state of health. |
Serology
| Agent | Assay | No. Pos./No. Tested | Comments |
|---|
| Sendai | MFI | 0/6 | |
| RCV/SDAV | MFI | 0/6 | |
| Parvoviruses | MFI | 0/6 | |
| KRV | MFI | 0/6 | |
| H-1 | MFI | 0/6 | |
| PVM | MFI | 0/6 | |
| M. pulmonis | MFI | 0/6 | |
| Reo 3 | MFI | 0/1 | |
| TMEV GDVII | MFI | 0/1 | |
| MAD | MFI | 0/1 | |
| LCM | MFI | 0/1 | |
| Hantaan | MFI | 0/1 | |
Bacteriology
| Agent | No. Pos./No. Tested | Comments |
|---|
| Mycoplasma pulmonis | 0/6 | |
| Salmonella spp. | 0/6 | Nasopharynx/lung/cecum/uterine cultures |
| Pasturella pneumotropica | 0/6 | |
| Pasturella spp. | 0/6 | |
| Bordetella bronchiseptica | 0/6 | |
| Pseudomonas aeruginosa | 0/6 | |
| Citrobacter rodentium | 0/6 | |
| Corynebaterium kutscheri | 0/6 | |
| Klebsiella pneumoniae | 0/6 | |
| Klebsiella oxytoca | 0/6 | The bacteria isolated from the nasopharynx and cecum are opportunistic or normal flora of the rat. |
| Staphylococcus aureus | 0/6 |
| Staphylococcus pneumoniae | 0/6 | |
| Streptococcus sp. Group B beta | 0/6 | |
PCR
| | No. Pos./No.Tested | Comments |
|---|
| Mycoplasma pulmonis | 0/6 | |
| Heliobacter hepaticus | 0/6 | |
Parasitology
| Type and Test | Results | Comments |
|---|
| Ectoparasites | No. Pos./No. Tested | External parasites were not observed in any rat. |
Test: Direct pelage
| 0/6 |
| Endoparasites | No. Pos./No. Tested | Enteric and urinary bladder parasites were not observed in any rat. |
Test(s): Direct, Gross, and Microscopic (intestines & bladder)
| 0/6 |
Pathology
| Gross: | None Significant | Overall frequency of lesions in these rats is low. |
|---|
| Histologic: | | |
|---|
| Lung lymphoid hyperplasia | 6/6 | Lesions are incidental and/or consistent with normal aging process and are not indicative of infection with murine adventitious pathogens. |
| Harderian gland lymphoid infiltrate | 2/6 |
| Nephrocalcinosis | 3/6 |
| Interstitial pneumonia | 1/6 |
| Myocardial degeneration | 3/6 | |
A few other incidental lesions were also noted. | | |
Availability
Rats obtained from NIA-NIH aging colonies maintained in a maximum security barrier by Harlan in Indianapolis, Indiana. The combined census of these colonies, at the time of the referenced monitoring, is tabulated below: |
Retired Breeder - Male (9-20 months of age) | 179 | |
Virgin - Male (1-31 months of age) | 4,990 | |
Virgin - Female (1-31 months of age)
| 2,709
| |
Total Census:
| 7,876 | |
For information, contact:
Dr. Karla A. Stevens
HARLAN SPRAGUE DAWLEY, INC.
8520 Allison Pointe, #400
Indianapolis, IN 46250
Phone: (317) 806-6060
Fax: (317) 806-6073
Signed by Karla A. Stevens, D.V.M., Principal Investigator
Page last updated Feb 19, 2009
