Harlan Animal Colonies Health Evaluation Summary: 9/14/07
Contract N01-AG-5-0006
RATS Barrier 218A
| Animal Facility | Strain | No./Sex | Age | Testing Laboratory/Dates |
|---|
| Location: Prattville, AL | BN/BiRijNNiaHsd | 1M/1F | 9-10 months | University of Missouri |
| Barrier/Building: 218A | | 2M/3F | 16-19 months | UMRADIL, Columbia, MO |
| | | | | |
| | | | | Receipt Date: 7/16/07 |
| Ship Date: 7/15/07 | F344 x BN F1 | 3M/4F | 4-5 months | Report Date: 9/11/07 |
Evaluation
| NIA rats submitted for this health monitoring were obtained from NIA-NIH aging colonies maintained in barriers by Harlan Sprague Dawley, Inc. All rats examined were considered to be in a good state of health. |
Serology
| Agent | Assay | No. Pos./No. Tested | Comments |
|---|
| Sendai | MFI | 0/14 | |
| RCV/SDAV | MFI | 0/14 | |
| Parvoviruses | MFI | 0/14 | |
| KRV | MFI | 0/14 | |
| H-1 | MFI | 0/14 | |
| PVM | MFI | 0/14 | |
| M. pulmonis | MFI | 0/14 | |
| Reo 3 | MFI | 0/4 | |
| TMEV GDVII | MFI | 0/4 | |
| MAD | MFI | 0/4 | |
| LCM | MFI | 0/4 | |
| E. cuniculli | MFI | 0/4 | |
| Hantaan | MFI | 0/4 | |
| Tyzzer's | MFI | 0/4 | |
Bacteriology
| Agent | No. Pos./No. Tested | Comments |
|---|
| Salmonella spp. | 0/14 | Nasopharynx/cecum cultures |
| Pasturella pneumotropica | 0/14 | |
| Pseudomonas aeruginosa | 0/0* | *No cetrimide or MacConkey plates were innoculated for the cecum. |
| Corynebaterium kutscheri | 0/14 |
| Klebsiella sp. | 0/4 | |
| Staphylococcus aureus | 0/4 | |
| Streptococcus pneumoniae | 0/14 | |
PCR
| | No. Pos./No.Tested | Comments |
|---|
| Mycoplasma pulmonis | 0/14 | |
| Heliobacter spp | 0/14 | |
Parasitology
| Type and Test | Results | Comments |
|---|
| Ectoparasites | No. Pos./No. Tested | External parasites were not observed in any rat. |
Test: Direct pelage
| 0/14 |
| Endoparasites | No. Pos./No. Tested | |
Test(s): Direct, Gross, and Microscopic
| 0/14 |
Pathology
| Gross: | No Gross Lesions Detected | |
|---|
| Histologic: | | |
|---|
Multifocal interstitial fibrosis with pigmented macrophages - lung | 4/14 | Lesions are incidental and/or consistent with normal aging process and are not indicative of infection with murine adventitious pathogens. |
Peribronchiolar lymphoid hyperplasia - lung | 6/14 |
A few additional incidental lesions were also noted. | | |
Availability
Rats obtained from NIA-NIH aging colonies maintained in one maximum security barrier by Harlan Sprague Dawley, Inc., (Harlan) in Prattville, Alabama. The combined census of these colonies, at the time of the referenced monitoring is tabulated below: |
Virgin Male (1-4 months of age) | 1,513 | |
Virgin Female (1-5 months of age)
| 335
| |
Total Census:
| 1,848 | |
For information, contact:
Dr. Karla Stevens
Harlan Sprague Dawley, Inc.
P.O. Box 29176
Indianapolis, IN 46229
Phone: (317) 894-7521
Fax: (317) 894-4473
Signed by Karla A. Stevens, D.V.M., Principal Investigator
Page last updated Feb 19, 2009
