National Toxicology Program

Alternative Ocular Safety Testing References: The Bovine Corneal Opacity and Permeability (BCOP) Test Method

References cited in Background Review Document

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• Bailey PT, Freeman JJ, Phillips RD, Merrill JC. 2004. Validation of the BCOP assay as a predictor of ocular irritation of various petrochemical products. Poster presentation at the Society of Toxicology 2004 meeting.
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• ICCVAM. 2006a. Background Review Document: Current Status of In Vitro Test Methods for Identifying Ocular Corrosives and Severe Irritants: Bovine Corneal Opacity and Permeability Test Method. NIH Publication No: 06-4512. Research Triangle Park: National Toxicology Program.
• ICCVAM. 2006b. ICCVAM Test Method Evaluation Report: In Vitro Ocular Toxicity Methods for Identifying Severe Irritants and Corrosives. NIH Publication No: 07-4517. Research Triangle Park: National Toxicology Program.
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• Southee JA. 1998. Evaluation of the Prevalidation Process. Part 2, final report. Volume 2. The Bovine Corneal Opacity and Permeability (BCOP) Assay. European Community Contract No. 11279-95-10F 1ED ISP GB.
• Swanson JE, Harbell JW. 2000. Evaluating the eye irritancy potential of ethanolic test materials with the bovine corneal opacity and permeability assay. The Toxicologist 54(1):188-189.
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• Van Goethem F, Adriaens E, Alepee N, Straube F, De Wever B, Cappadoro M, Catoire S, Hansen E, Wolf A, and Vanparys P. 2006. Prevalidation of a new in vitro reconstituted human cornea model to assess the eye irritating potential of chemicals. Toxicol in Vitro. 20:1-17.