Biological Pollutants in Ambient Water

Microbiological Measurements

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In September 2008, we completed two reports:

False positive and negative rates for fecal coliforms (EPA Methods 1680 and 1681) in biosolids (30 pp., 158 K)
Validation of enterococci (EPA Methods 1106.1 and 1600) and E. coli (EPA Methods 1103.1 and 1603) in CSO matrices (PDF) (66 pp., 347 K)

Guidelines Establishing Test Procedures for the Analysis of Pollutants

Analytical Methods for Biological Pollutants in Wastewater and Sewage Sludge

September 29, 2007

We issued new methods that use culture-based approaches to detect enterococci and Escherichia coli (E. coli) in wastewater. Additional tests will also identify Salmonella and fecal coliform bacteria in sewage sludge.

Fact Sheet
Final Rule (Federal Register Notice) (March 26, 2007)

Number	Method Title
1103.1	Escherichia coli (E. coli) in Water by Membrane Filtration Using membrane-Thermotolerant Escherichia coli Agar (mTEC) (PDF) (45 pp., 659 K)
1106.1	Enterococci in Water by Membrane Filtration Using membrane-Enterococcus-Esculin Iron Agar (mE-EIA) (PDF) (42 pp., 586 K)
1600	Membrane Filter Test Method for Enterococci in Water (PDF) (42 pp., 335 K)
1603	Escherichia coli (E. coli) in Water by Membrane Filtration Using Modified membrane-Thermotolerant Escherichia coli Agar (Modified mTEC) (PDF) (42 pp., 326 K)
1680	Fecal Coliforms in Sewage Sludge (Biosolids) by Multiple-Tube Fermentation using Lauryl Tryptose Broth (LTB) and EC Medium (PDF) (49 pp, 225 K)
1681	Fecal Coliforms in Sewage Sludge (Biosolids) by Multiple-Tube Fermentation using A-1 medium (PDF) (51 pp, 242 K)
1682	Salmonella in Sewage Sludge (Biosolids) by Modified Semisolid Rappaport-Vassiliadis (MSRV) Medium (PDF) (48 pp, 284 K)

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Other Biosolids Methods

Note: Many of these methods are drafts.

Other CWA Methods of Interest *not* Currently Approved for use at 40 CFR 136
Number	Method Title
200.7	Trace Elements in Water, Solids, and Biosolids by Inductively Coupled Plasma-Atomic Emission Spectrometry Revision 5.0 (PDF) (156 K, 68 pp.; EPA 821-R-01-010)
200.9	Trace Elements in Water, Solids, and Biosolids by Stabilized Temperature Graphite Furnace Atomic Absorption Spectrometry Revision 3.0 (PDF) (117 K, 51 pp.; EPA 821-R-01-011)
1656A	Organo-Halide Pesticides in Wastewater, Soil, Sludge, Sediment and Tissue by GC/HSD Revision A (PDF) (150 K, 58 pp.; EPA 821-R-00-017)
1657A	Organo-Halide Pesticides in Wastewater, Soil, Sludge, Sediment and Tissue by GC/FPD Revision A (PDF) (141 K, 52 pp.; EPA 821-R-00-018)
1680, Draft	Fecal Coliforms in Biosolids by Multiple-Tube Fermentation Procedures Draft, October 2002 (PDF) (202 K, 37 pp.; EPA 821-R-02-026)
1682, Draft	Salmonella spp. in Biosolids by Enrichment, Selection and Biochemical Characterization Draft, August 1998 (PDF) (99 K, 31 pp.; EPA 821-R-98-004)
1683, Draft	Specific Oxygen Uptake Rate in Biosolids Draft, January 2001 (PDF) (62 K, 23 pp.; EPA 821-R-01-014)
1684, Draft	Total, Fixed, and Volatile Solids in Water, Solids, and Biosolids Draft, January 2001 (PDF) (41 K, 16 pp.; EPA 821-R-01-015)
1685, Draft	Nitrate/Nitrite-N in Water and Biosolids by Automated Photometry Draft, January 2001 (PDF) (67 K, 28 pp.; EPA 821-R-01-016)
1686, Draft	Nitrate/Nitrite-N in Water and Biosolids by Manual Colorimetry Draft, January 2001 (PDF) (62 K, 26 pp.; EPA 821-R-01-005)
1687, Draft	Total Kjeldahl Nitrogen in Water and Biosolids by Automated Colorimetry with Preliminary Distillation/Digestion Draft, January 2001 (PDF) (68 K, 29 pp.; EPA 821-R-01-004)
1688, Draft	Total Kjeldahl Nitrogen in Water and Biosolids by Automated Colorimetry with Preliminary Semi-automatic Digestion Draft, January 2001 (PDF) (67 K, 28 pp.; EPA 821-R-01-007)
1689, Draft	Ammonia-N in Water and Biosolids by Ion-Selective Electrode Potentiometry with Preliminary Distillation Draft, January 2001 (PDF) (67 K, 25 pp.; EPA 821-R-01-012)
1690, Draft	Ammonia-N in Water and Biosolids by Automated Colorimetry with Preliminary Distillation Draft, January 2001 (PDF) (65 K, 27 pp.; EPA 821-R-01-009)