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Damage to DNA and DNA Monomers Mediated by Nonprotein Biological Complexes of Manganese(II)/(III) and Environmentally Available Oxidants

EPA Grant Number: U915358
Title: Damage to DNA and DNA Monomers Mediated by Nonprotein Biological Complexes of Manganese(II)/(III) and Environmentally Available Oxidants
Investigators: Jameton, Rachel
Institution: University of Utah
EPA Project Officer: Boddie, Georgette
Project Period: July 1, 1998 through June 1, 2001
Project Amount: $74,062
RFA: STAR Graduate Fellowships (1998)
Research Category: Academic Fellowships , Fellowship - Chemistry and Materials Science , Engineering and Environmental Chemistry

Description:

Objective:

The objective of this research project is to elucidate the role of nonprotein, biologically relevant manganese(II)/(III) complexes in DNA damage and/or protection when exposed to: (1) an intracellular oxidant (hydrogen peroxide); and (2) an environmentally available oxidant system (sulfite/oxygen).

Approach:

Manganese(II)-DNA reactivity in the presence of hydrogen peroxide and carbonate, in phosphate or pyrophosphate buffer, was examined by polyacrylamide gel electrophoresis (PAGE). Manganese(II)-DNA reactivity in the presence of sulfite also was examined by PAGE. Radicals generated in the system without DNA were detected by electron spin resonance (ESR) trapping experiments. To identify the DNA moiety at which the reaction occurs, the reaction of DNA monomers was investigated by ESR spin trapping, 31P nuclear magnetic resonance, and electrospray mass spectrometry.

Supplemental Keywords:

fellowship, DNA monomers, DNA, DNA damage, polyacrylamide gel electrophoresis, PAGE, manganese (II)/(III) complexes, oxidants. , Toxics, Scientific Discipline, Health, Toxicology, EPCRA, Disease & Cumulative Effects, HAPS, Analytical Chemistry, Health Risk Assessment, Environmental Microbiology, Environmental Chemistry, Chemistry and Materials Science, Contaminant Candidate List, molecular mechanisms, Manganese, tricarbonyl methylcyclopentadienyl, manganese toxicity, Manganese Compounds

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The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.

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