Pacific Southwest

Title: Analyzing genetic diversity in conifers...isozyme resolution by starch gel electrophoresis

Author: Conkle, M. Thompson

Date: 1972

Source: Res. Note PSW-RN-264. Berkeley, CA: U.S. Department of Agriculture, Forest Service, Pacific Southwest Forest and Range Experiment Station. 6 p

Station ID: RN-PSW-264

Description: Enzymes in forest tree materials can be resolved by starch gel electrophoresis. A gel stab is prepared in a mold assembled from glass and plastic. Wicks containing an aqueous extract of macerated plant material are inserted in the gel and processed. The gel is sliced, stained, examined, and photographed. Isozyme bands produced by differential migration of enzymes indicate genetic segregation and recombination. This technique is familiar in other uses, but the procedure described here is specifically adapted to conifers.

Keywords: coniferae; natural variation; genetic diversity; chemical composition; enzyme analysis; electrophoresis; techniques and procedures

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Citation

Conkle, M. Thompson  1972.  Analyzing genetic diversity in conifers...isozyme resolution by starch gel electrophoresis.   Res. Note PSW-RN-264. Berkeley, CA: U.S. Department of Agriculture, Forest Service, Pacific Southwest Forest and Range Experiment Station. 6 p.