Survival of <script type="text/javascript"> var wmNotice = "UNT Web Archive - External links, forms, and search boxes may not function within this collection. Url: http://www.treesearch.fs.fed.us/pubs/30283 time: 20:14:46 Dec 04, 2008"; var wmHideNotice = "hide"; var contextRoot = "https://webarchive.library.unt.edu/eot2008/"; </script> <script type="text/javascript" src="https://webarchive.library.unt.edu/eot2008/js/disclaim.js"></script> <i>Phytophthora ramorum</i> Chlamydospores at high and low temperatures

	This publication was written or produced by the Pacific Southwest Research Station and is in the public domain.

	FS INFO In depth catalog of natural resources publications, including index to Forest Service literature, late 1800's to date.

Publication Information

Title: Survival of Phytophthora ramorum Chlamydospores at high and low temperatures

Author: Tooley,Paul W.; Browning, Marsha

Date: 2008

Source: In: Frankel, Susan J.; Kliejunas, John T.; Palmieri, Katharine M., tech. coords. 2008. Proceedings of the sudden oak death third science symposium. Gen. Tech. Rep. PSW-GTR-214. Albany, CA: U.S. Department of Agriculture, Forest Service, Pacific Southwest Research Station. p. 475

Note: This article is part of a larger document. View the larger document

Station ID: GTR-PSW-214

Description: Chlamydospores were produced as described by Colburn and Shishkoff (Phytopathology 96:S25). Samples (5cc) of chlamydospores in sand inoculum were placed in 15 ml conical plastic test tubes and incubated at selected temperatures for 1, 2, 3, 4, and 7 days. Following incubation, tube contents were resuspended in 0.2 percent water agar and 1 ml was plated onto PARPH selective medium amended with 4 percent clarified V8 juice. Numbers of colonies resulting from germinated chlamydospores were assessed microscopically. High temperature treatments included 30, 35, and 40°C while low temperature treatments included 0, -10, and -20°C. All experiments also included chlamydospores placed at 20°C as positive controls. Near 100 percent survival was observed at temperatures of 0°C and for the 20°C controls for up to 7 days in the low temperature treatments, while almost no survival occurred at -10 or -20°C over the 7 day period. For the high temperature treatments, high levels of chlamydospore germination were observed over the 7 day period at 30°C and for the 20°C controls, while no growth was observed at 40°C. At 35°C, high levels of chlamydospore germination were observed at day 1, but growth declined steadily and was zero by 7 days. These results help define the temperature conditions under which chlamydospores of P. ramorum survive, and provide information to help define treatments aimed at inactivating chlamydospores in soil substrates.

Key Words: Phytophthora ramorum, chlamydospore survival

Publication Notes:

Citation

Tooley,Paul W.; Browning, Marsha 2008. Survival of Phytophthora ramorum Chlamydospores at high and low temperatures In: Frankel, Susan J.; Kliejunas, John T.; Palmieri, Katharine M., tech. coords. 2008. Proceedings of the sudden oak death third science symposium. Gen. Tech. Rep. PSW-GTR-214. Albany, CA: U.S. Department of Agriculture, Forest Service, Pacific Southwest Research Station. p. 475.