Pacific Southwest

Title: Molecular markers for identification of P. ramorum and other Phytophthora species from diseased tissue

Author: Martin, Frank N.; Tooley, Paul W.

Date: 2006

Source: In: Frankel, Susan J.; Shea, Patrick J.; and Haverty, Michael I., tech. coords. Proceedings of the sudden oak death second science symposium: the state of our knowledge. Gen. Tech. Rep. PSW-GTR-196. Albany, CA: Pacific Southwest Research Station, Forest Service, U.S. Department of Agriculture: 153-155

Station ID: GTR-PSW-196

Description: Molecular techniques have been developed for detection and identification of P. ramorum and other Phytophthora species that are based on the mitochondrially encoded sequences. One technique uses a Phytophthora genus specific primer to determine if a Phytophthora species is present, followed by a nested amplification with species-specific primers to determine if the pathogen is P. ramorum or one of several other species (such as P. nemorosa or P. pseudosyringe). This procedure also uses a plant primer pair to serve as a positive control ensuring the ability of the template DNA to amplify. These species-specific and plant markers have been modified for use with real-time PCR. A second technique relies on RFLP analysis of the Cox I and II gene cluster to identify isolates of Phytophthora to a species level.

Keywords: detection, identification, mitochondrial DNA, Phytophthora ramorum

View and Print this Publication (25 KB)

Publication Notes: 

• We recommend that you also print this page and attach it to the printout of the article, to retain the full citation information.
• This article was written and prepared by U.S. Government employees on official time, and is therefore in the public domain.
• You may send email to pubrequest@fs.f ed.us to request a hard copy of this publication. (Please specify exactly which publication you are requesting and your mailing address.)

 Get the latest version of the Adobe Acrobat reader or Acrobat Reader for Windows with Search and Accessibility

Citation

Martin, Frank N.; Tooley, Paul W.  2006.  Molecular markers for identification of P. ramorum and other Phytophthora species from diseased tissue.   In: Frankel, Susan J.; Shea, Patrick J.; and Haverty, Michael I., tech. coords. Proceedings of the sudden oak death second science symposium: the state of our knowledge. Gen. Tech. Rep. PSW-GTR-196. Albany, CA: Pacific Southwest Research Station, Forest Service, U.S. Department of Agriculture: 153-155.