Pacific Southwest

Title: Using single strand conformational polymorphisms (SSCP) to identify Phytophthora species in Oregon forests affected by sudden oak death

Author: Hansen, E.; Hesse, C.; Reeser, P.; Sutton, W.; Winton, L.

Date: 2006

Source: In: Frankel, Susan J.; Shea, Patrick J.; and Haverty, Michael I., tech. coords. Proceedings of the sudden oak death second science symposium: the state of our knowledge. Gen. Tech. Rep. PSW-GTR-196. Albany, CA: Pacific Southwest Research Station, Forest Service, U.S. Department of Agriculture: 141-142

Station ID: GTR-PSW-196

Description: Phytophthora species are abundant in streams, widespread in soils and occasionally found in diseased plants in the tanoak forests of southwestern Oregon. It is time-consuming and expensive to identify hundreds of isolates to species using morphology or internal transribed spacer (ITS) sequencing. We modified a published Phytophthora single strand conformation polymorphism (SSCP) protocol (Kong and others 2003) to use fluorescent-labeling chemistry and an additional marker locus to allow quantitative matching of unknown isolates.

Keywords: Phytophthora ramorum, Phytophthora species identification, sudden oak death

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Citation

Hansen, E.; Hesse, C.; Reeser, P.; Sutton, W.; Winton, L.  2006.  Using single strand conformational polymorphisms (SSCP) to identify Phytophthora species in Oregon forests affected by sudden oak death.   In: Frankel, Susan J.; Shea, Patrick J.; and Haverty, Michael I., tech. coords. Proceedings of the sudden oak death second science symposium: the state of our knowledge. Gen. Tech. Rep. PSW-GTR-196. Albany, CA: Pacific Southwest Research Station, Forest Service, U.S. Department of Agriculture: 141-142.