Product Pathways - Akt Signaling
Phospho-Tuberin/TSC2 (Thr1462) (5B12) Rabbit mAb #3617
Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
---|---|---|---|---|
W IP | H M R | Endogenous | 200 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by Western blot.
Protocols
Specificity / Sensitivity
Phospho-Tuberin/TSC2 (Thr1462) (5B12) Rabbit mAb detects endogenous levels of tuberin only when phosphorylated at threonine 1462.
Source / Purification
Monoclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Thr1462 of human tuberin.
Background
Tuberin, a product of the tumor suppressor gene TSC2, is important in regulating cell proliferation and tumor development (1). Mutations in either TSC1 or TSC2 are responsible for tuberous sclerosis complex (TSC), an autosomal dominant disorder (2). Tuberin is directly phosphorylated at Thr1462 by Akt/PKB (3). Phosphorylation at Thr1462 and Tyr1571 regulates tuberin-hamartin complexes and tuberin activity (3-5). In addition, tuberin inhibits the mammalian target of rapamycin (mTOR), resulting in inhibition of p70 S6 kinase and activation of eukaryotic initiation factor 4E binding protein 1 (4E-BP1, an inhibitor of translation initiation) and thus, inhibits translation (3,6,7).
- Soucek, T. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 15653-15658.
- Sparagana, S.P. and Roach, E.S. (2000) Curr. Opin. Neurol. 13, 115-119.
- Manning, B. D. et al. (2002) Mol. Cell 10, 151-161.
- Aicher, L. D. et al. (2001) J. Biol. Chem. 276, 21017-21021.
- Dan, H. C. et al. (2002) J. Biol. Chem. 277, 35364-35370.
- Goncharova, E.A. et al. (2002) J. Biol. Chem. 277, 30958-30967.
- Inoki, K. et al. (2002) Nat. Cell Biol. 4, 648-657.
Application References
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Companion Products
- 3611 Phospho-Tuberin/TSC2 (Thr1462) Antibody
- 3612 Tuberin/TSC2 Antibody
- 3614 Phospho-Tuberin/TSC2 (Tyr1571) Antibody
- 3615 Phospho-Tuberin/TSC2 (Ser939) Antibody
- 3616 Phospho-Tuberin/TSC2 (Ser1254) Antibody
- 4906 Hamartin/TSC1 Antibody
- 4963 Hamartin/TSC1 (1B2) Mouse mAb
- 6476 SignalSilence® Tuberin/TSC2 siRNA I
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- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.