goat polyclonal IgG, 200 µg/ml; also available as rabbit IgG, 200 µg/ml, sc-11769-R
recommended for detection of Ser 423 and Ser 425 dually phosphorylated Smad3 and correspondingly phosphorylated Smad2 of m, r and h origin by WB, IP, IF, IHC(P) and ELISA
blocking peptide, sc-11769 P
TransCruz reagent for Gel Supershift and ChIP applications, sc-11769 X, 200 µg/0.1 ml
Artaza, J.N. et al. 2008 . Myostatin promotes a fibrotic phenotypic switch in multipotent C3H 10T1/2 cells without affecting their differentiation into myofibroblasts. J Endocrinol. . 196: 235-249. View PubMed Entry
Robertson, NJ. et al. 2007. Embryonic stem cell-derived tissues are immunogenic but their inherent immune privilege promotes the induction of tolerance. Proc. Natl. Acad. Sci. USA. 104: 20920-20925. View PubMed Entry
Yanagita, M. et al. 2006. Uterine sensitization-associated gene-1 (USAG-1), a novel BMP antagonist expressed in the kidney, accelerates tubular injury. J. Clin. Invest. 116(1: 70-79. View PubMed Entry
Tobimatsu, T. et al. 2006 . Parathyroid hormone increases beta-catenin levels through Smad3 in mouse osteoblastic cells. Endocrinology. 147(5): 2583-2590. View PubMed Entry
Sun, Y. et al. 2006. Potentiation of Smad-mediated transcriptional activation by the RNA-binding protein RBPMS. Nucleic Acids Res. 34(21): 6314-6326. View PubMed Entry
p-Smad2/3 (Ser 423/425) Data
Click on image to enlarge
p-Smad2/3 (Ser 423/425): sc-11769. Immunoperoxidase staining of human embryonic skin (A) and bone (B) tissues showing nuclear localization. Kindly provided by Dr. Xiao-Mei Cui, University of Southern California.
Western blot analysis of Smad2/3 phosphorylation in untreated (A,C) and TGFβ-treated Mv 1 Lu (B,D) whole cell lysates. Antibodies tested include Smad1/2/3 (H-2): sc-7960 (A,B) and p-Smad2/3 (Ser 433/435)-R (C,D): sc-11769-R.
Western blot analysis of Smad2/3 phosphorylation in untreated (A,C) and TGFβ-treated Mv1Lu (B,D) whole cell lysates. Antibodies tested include Smad1/2/3 (H-2): sc-7960 (A,B) and p-Smad2/3 (Ser 423/425)-R (C,D): sc-11769-R.
