Anti-Smad2, phospho-specific (Ser465/467)
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | IF, WB, IH(P) | Rabbit | Affinity Purified | Polyclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: Q15796 # Transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase. SMAD2 is a receptor-regulated SMAD (R-SMAD). May act as a tumor suppressor in colorectal carcinoma.
SIZE: 467 amino acids; 52306 Da
SUBUNIT: Found in a complex with SMAD3 and TRIM33 upon addition of TGF-beta. Interacts with SMAD3 and TRIM33. Interacts with SARA (SMAD anchor for receptor activation); may form trimers with the SMAD4 co-SMAD. Interacts wi ... see more »
SIZE: 467 amino acids; 52306 Da
SUBUNIT: Found in a complex with SMAD3 and TRIM33 upon addition of TGF-beta. Interacts with SMAD3 and TRIM33. Interacts with SARA (SMAD anchor for receptor activation); may form trimers with the SMAD4 co-SMAD. Interacts wi ... see more »
Entrez Gene Number:
Entrez Gene Summary
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiatio
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Description:
Anti-Smad2, phospho-specific (Ser465/467)
Background Information:
SMAD2 or Mothers against decapentaplegic homolog 2 is a polypeptide that, as its name describes, is a homolog of the Drosophila gene: "Mothers against decepentaplegic". It belongs to the SMAD family of proteins, which belong to the TGFβ superfamily of modulators. Like many other TGFβ family members SMAD2 is involved in cell signalling. SMAD2 modulates signals of activin and TGFβ's. It interacts with SMAD anchor for receptor activation (SARA). The binding of ligands causes the phosphorylation of the SMAD2 protein and the dissociation from SARA and the association with SMAD4. It is subsequently transferred to the nucleus where it forms complexes with other proteins and acts as a transcription factor. SMAD2 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase.
Specificity:
By western blot, the antibody recognizes a band migrating at approximately 58 kDa corresponding to Smad2 only when dually phosphorylated at Ser465 and Ser467. The antibody may also detect phosphorylated Smad3 (Ser423 and Ser425). This antibody does not cross-react with other Smad-related proteins.
Key Applications:
- Immunofluorescence
- Western Blotting
- Immunohistochemistry (Paraffin)
Application Notes:
Western blotting: 1:1,000. For best results, incubate membrane with diluted antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C overnight with gentle agitation.
Immunohistochemistry (paraffin): 1:2000
Immunocytochemistry (IF): 1:500
Optimal working dilutions must be determined by the end user.
Immunohistochemistry (paraffin): 1:2000
Immunocytochemistry (IF): 1:500
Optimal working dilutions must be determined by the end user.
Species Reactivity:
- Human
- Mouse
- Rat
Immunogen:
KLH-conjugated, synthetic phospho-peptide corresponding to residues surrounding Ser465/467 of human Smad2. The antibody is predicted to react with chicken, rat, Xenopus, and zebrafish due to 100% sequence similarity.
Epitope:
phospho-specific (Ser465/467)
Modifications:
Phosphorylation
Molecular Weight:
58 kDa
Control:
Positive Control: TGFbeta treated HepG2 cells
Presentation:
Protein A and peptide affinity Purified immunoglobulin. Liquid in 10 mM sodium HEPES, pH 7.5, 150 mM NaCl, 100 µg/mL BSA, with 50% glycerol.
Storage Conditions:
Maintain at -20°C for up to 12 months from date of receipt. Do not aliquot.
Gene Symbol:
- SMAD2
- hMAD-2
- JV18
- hSMAD2
- MGC22139
- MADH2
- MGC34440
- MADR2
- JV18-1
Antibody Category:
Nuclear Function
Antibody Sub-Category:
Transcription Factors
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Descriptive Text:
Concentration:
Variable
Format Code:
APur
Trade Name:
Chemicon (Millipore)
Purification Method:
Immunoaffinity Purified
Format:
Affinity Purified
Antibody Type:
Polyclonal Antibody
Host:
Rabbit