Product Pathways - MAPK Signaling

Phospho-c-Jun (Ser73) Antibody #9164

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Applications	Reactivity	Sensitivity	MW (kDa)	Source
W IHC-P IF-IC	H M R	Endogenous	48	Rabbit

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by Western blot.

Protocols

9164:

IHC / Paraffin, Immunofluorescence, Western Blotting

Specificity / Sensitivity

Phospho-c-Jun (Ser73) Antibody detects endogenous levels of c-Jun only when activated by phosphorylation at Ser73. This antibody also recognizes JunD phosphorylated at Ser100.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phospho-peptide (KLH-coupled) corresponding to residues around Ser73 of human c-Jun. Antibodies are purified by protein A and peptide affinity chromatography.

Background

c-Jun is a member of the Jun Family containing c-Jun, JunB and JunD, and is a component of the transcription factor AP-1 (activator protein-1). AP-1 is composed of dimers of Fos, Jun and ATF family members and binds to and activates transcription at TRE/AP-1 elements (Reviewed in 1).Extracellular signals including growth factors, chemokines and stress activate AP-1-dependent transcription. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73 through SAPK/JNK (reviewed in 2). Knock-out studies in mice have shown that c-Jun is essential for embryogenesis (3), and subsequent studies have demonstrated roles for c-Jun in various tissues and developmental processes including axon regeneration (4), liver regeneration (5) and T cell development (6).AP-1 regulated genes exert diverse biological functions including cell proliferation, differentiation, and apoptosis, as well as transformation, invasion and metastasis, depending on cell type and context (7-9 ). Other target genes regulate survival as well as hypoxia and angiogenesis (8,10). c-Jun has emerged as a promising therapeutic target for cancer, vascular remodeling, acute inflammation, as well as rheumatoid arthritis (11-13).

1. Jochum, W. et al. (2001) Oncogene 20, 2401-12.
2. Davis, R.J. (2000) Cell 103, 239-52.
3. Hilberg, F. et al. (1993) Nature 365, 179-81.
4. Raivich, G. et al. (2004) Neuron 43, 57-67.
5. Behrens, A. et al. (2002) EMBO J 21, 1782-90.
6. Riera-Sans, L. and Behrens, A. (2007) J Immunol 178, 5690-700.
7. Leppa, S. and Bohmann, D. (1999) Oncogene 18, 6158-62.
8. Shaulian, E. and Karin, M. (2002) Nat Cell Biol 4, E131-6.
9. Weiss, C. and Bohmann, D. (2004) Cell Cycle 3, 111-3.
10. Karamouzis, M.V. et al. (2007) Mol Cancer Res 5, 109-20.
11. Kim, S. and Iwao, H. (2003) J Pharmacol Sci 91, 177-81.
12. Weiss, C. and Bohmann, D. (2004) Cell Cycle 3, 111-3.
13. Dass, C.R. and Choong, P.F. (2008) Pharmazie 63, 411-4.

Application References

• de Ruiter, N. D. et al. (2000) Ras-dependent regulation of c-Jun phosphorylation is mediated by the ral guanine nucleotide exchange factor-Ral pathway. Mol. Cell. Biol. 20, 8480-8488. This article references the use of Phospho-c-Jun (Ser73) Antibody in the following applications: Western Blotting
• Fan, M. et al. (2000) Vinblastine-induced phosphorylation of bcl-2 and bcl-XL is mediated by JNK and occurs in parallel with inactivation of the raf-1/MEK/ERK cascade. J. Biol. Chem. 275, 29980-29985. This article references the use of Phospho-c-Jun (Ser73) Antibody in the following applications: Western Blotting
• Lee, S. A. et al. (2001) Role of ATM in oxidative stress-mediated c-Jun phosphorylation in response to ionizing radiation and CdCl2. J. Biol. Chem. 276, 11783-11790. This article references the use of Phospho-c-Jun (Ser73) Antibody in the following applications: Western Blotting
• Paasinen-Sohns, A. et al. (2000) c-Jun activation-dependent tumorigenic transformation induced paradoxically by overexpression or block of S-adenosylmethionine decarboxylase. J. Cell Biol. 151, 801-810. This article references the use of Phospho-c-Jun (Ser73) Antibody in the following applications: Western Blotting
• Hirai, S. et al. (2006) J Neurosci 26, 11992-2002. This article references the use of Phospho-c-Jun (Ser73) Antibody in the following applications: Western Blotting

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

• 1030 Phospho-c-Jun (Ser73) Blocking Peptide
• 9260 PhosphoPlus® c-Jun (Ser63) II and c-Jun (Ser73) Antibody Kit
• 9261 Phospho-c-Jun (Ser63) II Antibody
• 9165 c-Jun (60A8) Rabbit mAb
• 9263 c-Jun Control Cell Extracts
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 7071 Phototope^®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.