Product Pathways - MAPK Signaling
Phospho-SEK1/MKK4 (Ser257/Thr261) Antibody #9156
Applications | Reactivity | Sensitivity | MW (kDa) | Source |
---|---|---|---|---|
W | H M R Dm (X) (Z) | Endogenous | 44 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Dm=D. melanogaster
X=Xenopus
Z=Zebrafish
Species cross-reactivity is determined by Western blot.
Protocols
- 9156:
- Western Blotting
Specificity / Sensitivity
Phospho-SEK1/MKK4 (Ser257/Thr261) Antibody detects endogenous levels of SEK1/MKK4 phosphorylated at serine 257/threonine 261 . This antibody does not cross-react with the corresponding phosphorylated residues of MEK1, MEK2 or MKK3.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH coupled) corresponding to residues surrounding Ser257/Thr261 of human SEK1/MKK4. Antibodies are purified by protein A and peptide affinity chromatography.
Background
SAPK/Erk kinase (SEK1), also known as MKK4 or Jun kinase kinase (JNKK), activates the MAP kinase homologues SAPK and JNK in response to various cellular stresses and inflamatory cytokines (1-3). Activation of SEK1 occurs through MEKK phosphorylation of serine and threonine residues at positions 257 and 261, respectively. Like MEK, SEK is a dual-specificity protein kinase that phosphorylates SAPK/JNK at a conserved T*PY* site in its activation loop (4). Phosphorylation by Akt at Ser80 inhibits SEK1 and suppresses stress-activated signal transduction (5).
- Davis, R.J. (1994) Trends Biochem. Sci. 19, 470-473.
- Sanchez, I. et al. (1994) Nature 372, 794-798.
- Yan, M. et al. (1994) Nature 372, 798-800.
- Kyriakis, J.M. et al. (1994) Nature 369, 156-160.
- Park, H. et al. (2002) J. Biol. Chem. 277, 2573-2578.
Application References
- Zapata, H.J. et al. (2007) J Virol 81, 977-90. This article references the use of Phospho-SEK1/MKK4 (Ser257/Thr261) Antibody in the following applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 3346 SEK1 (5C10) Rabbit mAb
- 4514 Phospho-SEK1/MKK4 (Ser257) (C36C11) Rabbit mAb
- 9151 Phospho-SEK1/MKK4 (Thr261) Antibody
- 9152 SEK1/MKK4 Antibody
- 9155 Phospho-SEK1/MKK4 (Ser80) Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.