Product Pathways - Tyrosine Kinase/ Adaptors
Phospho-VEGF Receptor 2 (Tyr996) Antibody #2474
Applications | Reactivity | Sensitivity | MW (kDa) | Source |
---|---|---|---|---|
W | H M | Transfected Only | 230 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
Species cross-reactivity is determined by Western blot.
Protocols
- 2474:
- Western Blotting
Specificity / Sensitivity
Phospho-VEGF Receptor 2 (Tyr996) Antibody detects transfected VEGFR 2 only when phosphorylated at tyrosine 996. The antibody cross-reacts with tyrosine-phosphorylated PDGF receptor.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Tyr996 of human VEGF receptor 2. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
![Western Blotting](images/2474_wb_ka_080821.jpg)
Western blot analysis of extracts from CKR/PAE cells expressing chimeric receptors containing human CSF-1 extracellular binding domain/mouse VEGF receptor 2 intracellular domains (Rahimi, N. et al. (2000) J. Biol. Chem. 275, 16986-16992), using Phospho-VEGF Receptor 2 (Tyr996) Antibody (upper) or VEGF receptor 2 antibody (lower).
Background
Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059) (2). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, GRB2, PI-3 kinase, Nck and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). The phosphorylation of Tyr1212 provides a docking site for Grb2 binding and phospho-Tyr1175 binds with the p85 subunit of PI-3 kinase and PLCγ, as well as Shb (1,4,5). Signaling from VEGFR2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and sprouting, as well as survival of cultured endothelial cells in vitro and angiogenesis in vivo (6-8).
- Meyer, M. et al. (1999) EMBO J. 18, 363-374.
- Dougher-Vermazen, M. et al. (1994) Biochem. Biophys. Res. Commun. 205, 728-738.
- Kroll, J. and Waltenberger, J. (1997) J. Biol. Chem. 272, 32521-32527.
- Takahashi, T. et al. (2001) EMBO J. 20, 2768-2778.
- Holmqvist, K. et al. (2004) J. Biol. Chem. 279, 22267-22275.
- Karkkainen, M.J. and Petrova, T. (2000) Oncogene 19, 5598-5605.
- Rahimi, N. et al. (2000) J. Biol. Chem. 275, 16986-16992.
- Claesson-Welsh, L. (2003) Biochem. Soc. Trans. 31, 20-24.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 2904 VEGF Receptor 2 Control Proteins
- 9943 Vascular Endothelial Growth Factor (VEGF)
- 2478 Phospho-VEGF Receptor 2 (Tyr1175) (19A10) Rabbit mAb
- 2479 VEGF Receptor 2 (55B11) Rabbit mAb
- 4991 Phospho-VEGF Receptor 2 (Tyr951) (15D2) Rabbit mAb
- 2477 Phospho-VEGF Receptor 2 (Tyr1212) (11A3) Rabbit mAb
- 2471 Phospho-VEGF Receptor 2 (Tyr951) Antibody
- 2472 VEGF Receptor 2 Antibody
- 2476 Phospho-VEGF Receptor 2 (Tyr951) (7H11) Mouse mAb
- 2893 VEGF Receptor 1 Antibody
- 2638 VEGF Receptor 3 (C28G5) Rabbit mAb
- 2485 VEGF Receptor 3 Antibody
- 2463 VEGF-B Antibody
- 2445 VEGF-C Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.