Clone ID: E377
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 1532-1
Size: 100ul
Price: $ 285
Recommended Applications: WB, ICC
Species Reactivity: Human, Mouse, Rat
Mol. Weight: 74 kDa
UniProt ID: P17600
Alternative names: Synapsin I, Brain protein 4.1
Description: Synapsin I is a member of neuron-specific phosphoproteins that associate with synaptic vesicles (1). Three mammalians synapsins have been identified, Synapsin I, II and III. Generated by differential splicing, Synapsin Ia and Synapsin Ib differ in their Carboxyl termini (2). Synapsin I also functions in binding synaptic vesicles to several elements of the cytoskeleton including actin filaments, microtubules and spectrin. (3) Fairly abundant protein of the brain (0.4%), Synapsin I is best known to be CaM kinase II substrate in addition to cAMP-dependent protein kinase. (4) Phosphorylation at site Ser 566 and Ser 603 by CaM kinase II will cause a 5 fold decrease in the affinity of synaptic vesicles binding (5). Specificity: A synthetic phospho-peptide corresponding to residues surrounding Ser553 of human Synapsin was used as immunogen. The antibody only detects Synapsin phosphorylated on Serine 553.
Recommended Dilutions:
WB: 1:10,000-50,000
ICC: 1:50-100
Protocols for using Epitomics Rabbit Monoclonal Antibodies
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| A. Western blot analysis on mouse brain lysates using anti-Synapsin (pS553) RabMAb (catalog #1532-1), dilution 1:50,000 |
B. Immunofluorescent staining of PC-12 cells using anti-Synapsin (pS553) RabMAb (catalog #1532-1) |
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| C. Dot Blot analysis on immunogen phospho-peptide (A) and non-phospho peptide (B) using anti-Synapsin (pS553) RabMAb (cat. # 1532-1) dilution 1:500 |
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Description References:
| 1. |
Greengard P, Valtorta F, Czernik AJ, Benfenati F (1993) Science 259:780 |
| 2. |
S |
| 3. |
Bahler M, Greengard P (1987) Nature 326:704 |
| 4. |
Huttner WB, DeGennaro LJ, Greengard P (1981) J Biol Chem 256:1482 |
| 5. |
Schiebler, W., Jahn, R., Doucet, J-P Rothlein, J., and Greengard, P. (1986) J. Biol Chem. 261, 8383-8390 |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
