Product Pathways - Cytoskeletal Signaling
Phospho-Troponin I (Cardiac) (Ser23/24) Antibody #4004
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R | Endogenous | 28 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by Western blot.
Protocols
- 4004:
- Western Blotting
Specificity / Sensitivity
Phospho-Troponin I (Cardiac) (Ser23/24) Antibody detects endogenous levels of cardiac troponin I only when phosphorylated at Ser23/24.This antibody does not cross-react with phosphorylated skeletal muscle troponin I.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser23/24 of human troponin I (cardiac). Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from mouse myocytes, untreated or stimulated with isoproterenol, using Phospho-Troponin I (Cardiac) (Ser23/24) Antibody (upper) or Troponin I Antibody #4002 (lower).
Background
Troponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration. Troponin consists of three subunits, the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI) (1). In response to beta-adrenergic stimulation of the heart, Ser23 and Ser24 of TnI (cardiac) are phosphorylated by PKA and PKC. This phosphorylation stimulates a conformational change of the regulatory domain of TnC, reduces the association between TnI and TnC, and decreases myofilament Ca2+ sensitivity by reducing the Ca2+ binding affinity of TnC (1-3).
- Ward, D.G. et al. (2002) J. Biol. Chem. 277, 41795-41801.
- Noland, T.A. et al. (1995) J. Biol. Chem. 270, 25445-25454.
- Gaponenko, V. et al. (1999) J. Biol. Chem. 274, 16681-16684.
Application References
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Companion Products
- 4002 Troponin I Antibody
- 9621 Phospho-(Ser/Thr) PKA Substrate Antibody
- 2261 Phospho-(Ser) PKC Substrate Antibody
- 9921 Phospho-PKC Antibody Sampler Kit
- 9371 Phospho-PKC (pan) (βII Ser660) Antibody
- 9374 Phospho-PKCdelta (Thr505) Antibody
- 9375 Phospho-PKCalpha/beta II (Thr638/641) Antibody
- 9376 Phospho-PKCδ/θ (Ser643/676) Antibody
- 9379 Phospho-PKC (pan) (gamma Thr514) Antibody
- 2055 Phospho-PKCdelta (Tyr311) Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
