Product Pathways - Neuroscience
Phospho-delta-Opioid Receptor (Ser363) Antibody #3641
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IF-F IF-IC | H M | Transfected Only | 50 to 65 | Rabbit |
Applications Key:
W=Western Blotting
IF-F=Immunofluorescence (Frozen)
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
M=Mouse
Species cross-reactivity is determined by Western blot.
Protocols
Specificity / Sensitivity
Phospho-delta-Opioid Receptor (Ser363) Antibody detects transfected delta-opioid receptor only when phosphorylated at serine 363.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH coupled) corresponding to residues around serine 363 of human delta-opioid receptor. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from flag-tagged delta-opioid receptor stably transfected 293 cells untreated (lane 1), treated with 0.1 µM DADLE (lane 2), 1 µM MENK (lane 3) or 1 µM DPDPE (lane 4) for 30 minutes each, using Phospho-delta-Opioid Receptor (Ser363) Antibody (upper) or flag-tagged antibody (lower).
IF-IC
Immunofluorescent staining of flag-tagged delta-opioid receptor stably transfected 293 cells untreated (A) or treated with 0.1 µM DADLE (B, C, D), using Phospho-delta-Opioid Receptor (Ser363) Antibody alone (A, B) or the same antibody preincubated with the corresponding nonphospho-antigenic peptide (C) or phospho-antigenic peptide (D).
Background
Phosphorylation is an essential signal involved in desensitization and internalization of G-protein-coupled receptors (GPCRs) (1). The opioid receptors, like other GPCRs, are typically phosphorylated at multiple sites upon agonist binding (2,3). However, little is known about the specific role of each phosphorylation site in GPCR desensitization and internalization or about how the kinetics of phosphate addition affects these important processes. Phosphorylation of the delta-opioid receptor (DOR) is hierarchical and involves at least two sites: threonine 358 and serine 363 (4). Serine 363 appears to be the primary phosphorylation site induced by the DOR-specific agonist DPDPE (4). Phosphorylation of DOR at serine 363 correlates with receptor internalization (4).
- Claing, A. et al. (2002) Prog. Neurobiol. 66, 61-79.
- Arden, J. R. et al. (1995) J. Neurochem. 65, 1636-1645.
- Pei, G. et al. (1995) Mol. Pharmacol. 48, 173-177.
- Maestri-El Kouhen, O. et al. (2000) J. Biol. Chem. 275, 36659-36664.
Application References
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
