Product Pathways - Lymphocyte Signaling
Phospho-Zap-70 (Tyr493) Antibody #2704
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP F | H (M) | Endogenous | 70 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
Species cross-reactivity is determined by Western blot.
Protocols
- 2704:
- Flow, Immunoprecipitation, Western Blotting
Specificity / Sensitivity
Phospho-Zap-70 (Tyr493) Antibody detects endogenous levels of Zap-70 only when phosphorylated at Tyr493.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Tyr493 of human Zap-70. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from Jurkat cells starved for 16 hours and treated with 2 mM H2O2 for 3 minutes, using Phospho-Zap-70 (Tyr493) Antibody (upper) or control Zap-70 Antibody #2702 (lower).
Flow Cytometry
Flow cytometric analysis of Jurkat cells, untreated (blue) or CD3/CD28 treated (green), using Phospho-Zap-70 (Tyr493) Antibody compared to a nonspecific negative control antibody (red).
Background
The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement (1). Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck (2-6). Tyrosine phosphorylation correlates with its increased Zap-70 kinase activity and downstream signaling events. Expression of Zap-70 is correlated with disease progression and survival in patients with chronic lymphocytic leukemia (CLL) (7,8).
Phosphorylation of Tyr493 within the activation loop results in enzymatic activation of Zap-70 (9).
- Chu, D.H. et al. (1998) Immunol. Rev. 165, 167-180.
- Iwashima, M. et al. (1994) Science 263, 1136-1139.
- Neumeister, E.N. et al. (1995) Mol. Cell Biol. 15, 3171-3178.
- Chan, A.C. et al. (1995) EMBO J. 14, 2499-2508.
- Williams, B.L. et al. (1999) EMBO J. 18, 1832-1844.
- Di Bartolo, V. et al. (1999) J. Biol. Chem. 274, 6285-6294.
- Wiestner, A. et al. (2003) Blood 101, 4944-4951.
- Crespo, M. et al. (2003) N. Engl. J. Med. 348, 1764-1775.
- Watts, J.D. et al. (1994) J. Biol. Chem. 269, 29520-29529.
Application References
- Zeyda, M. et al. (2002) LAT Displacement from Lipid Rafts as a Molecular Mechanism for the Inhibition of T Cell Signaling by Polyunsaturated Fatty Acids. J. Biol. Chem. 277, 28418-28423. This article references the use of Phospho-Zap-70 (Tyr493) Antibody in the following applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 2701 Phospho-Zap-70 (Tyr319)/Syk (Tyr352) Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 2705 Zap-70 (99F2) Rabbit mAb
- 2709 Zap-70 (L1E5) Mouse mAb
- 2717 Phospho-Zap-70 (Tyr319)/Syk (Tyr352) (65E4) Rabbit mAb
- 2707 Zap-70 (136F12) Rabbit mAb (Alexa Fluor® 647 Conjugate)
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
