Name: JNK1 (pY185) / JNK2 (pY185) /JNK3 (pY223) Rabbit Antibody
PDF of Datasheet |
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Clone ID: EP1597Y
Antibody Type: Rabbit Monoclonal IgG
Catalog#: 2155-1
Size: 100ul
Price: $ 285
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| A. Western blot analysis on Hek293 cell lysates using anti-JNK3 Phospho (pS223) RabMAb (cat. #2155-1), 1:20,000 dilution. Cells were either (A) untreated (B) treated with UV. |
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Recommended Applications: WB, IP
Species Reactivity: Human, Mouse, Rat
Mol. Weight: 46 kDa
UniProt ID: P53779
Alternative names: Stress-activated protein kinase JNK3, c-Jun N-terminal kinase 3, MAP kinase p49 3F12
Description: C-Jun N-terminal kinases (JNK) are distantly related to mitogen-activated protein kinases (ERKs) and are activated by dual phosphorylation on Tyr and Thr. The JNK protein kinase group consists of three isoforms (JNK1, 2, and 3) and regulates transcription activity of the AP-1 complex, and release and regulation of cytochome c (1). JNK becomes activated in vivo in response to pro-inflammatory cytokines or cellular stresses. Its full activation requires the phosphorylation of a threonine and a tyrosine residue in a Thr-Pro-Tyr motif, which can be catalysed by the protein kinases mitogen-activated protein kinase kinase MKK4 and MKK7 (2). Unlike other JNK isoforms, JNK3 is only expressed in heart and nervous system nervous. JNK3 has been implicated in stress-induced neuronal apoptosis (3). Specificity: A synthetic phospho-peptide corresponding to residues surrounding tyrosine 223 of human JNK3 was used as immunogen. The antibody will detect JNK1 (pY185)/JNK2(pY185)/JNK3 (pY223) phospholyation sites.
Recommended Dilutions:
WB: 1:5000 - 20000
IP: 1:20
Protocols for using Epitomics Rabbit Monoclonal Antibodies
Description References:
| 1. |
Kuan CY, et al. PNAS 100(25):15184-89, 2003 |
| 2. |
Fleming Y, et al. Biochem J 352(Pt1):145-54, 2000 |
| 3. |
Gupta S, et al. EMBO J. 15:2760-2770, 1996. |
Orders:
877-772-2622 (US and Canada)
650-583-6688 (Domestic and International)
orders@epitomics.com
Technical Support: support@epitomics.com
Product MSDS: view page or download PDF
*Determined species reactivity – for other species not listed cross reactivity
may be possible but not tested. |
