National Toxicology Program

CASRN: 88-72-2

Formula: C7-H7-N-O2

Synonyms/Common Names:

• 1-METHYL-2-NITROBENZENE (9CI)
• 2-NITROTOLUENE

Known Uses:

INTERMEDIATE FOR TOLUIDINES, ORGANIC SYNTHESIS OF PESTICIDES, PHARMACEUTICALS, MFR OF DYES, RUBBER CHEMICALS

Short-Term Toxicity

• 1-WEEK (Dosed-Feed)  (C62340C) Completed 
  • TOX-44 (NTIS No. PB96-188321)  (Toxicity Peer Review 08/00/1995)
    Comparative Toxicity and Carcinogenicity Studies ofo-Nitrotoluene (CAS No. 88-72-2) ando-Toluidine Hydrochloride (CAS No. 636-21-5) Administered in Feed to Male F344/N Rats
  • Rats: F344/N
• 2-WEEK (Dosed-Feed)  (C62340) Completed 
  • TOX-23 (NTIS No. PB93-150092)  (Peer Review 11/21/1991)
    Toxicity Studies ofo-,m-, andp- Nitrotoluenes (CAS Nos. 88-72-2, 99-08-1, 99-99-0) Administered in Dosed Feed to F344/N Rats and B6C3F1Mice
  • Rats: F344/N; Mice: B6C3F1
• 26-WEEK (Dosed-Feed)  (C62340C) Report Complete 
  • TOX-44 (NTIS No. PB96-188321)  (Toxicity Peer Review 08/00/1995)
    Comparative Toxicity and Carcinogenicity Studies ofo-Nitrotoluene (CAS No. 88-72-2) ando-Toluidine Hydrochloride (CAS No. 636-21-5) Administered in Feed to Male F344/N Rats
  • Rats: F344/N
  • Dose: MALE R: 0, 0 ALTERED MICROFLORA 20/GRP; 5000 PPM 60/GRP; 5000 PPM ALTERED MICROFLORA 40/GRP
• 13-WEEK (Dosed-Feed)  (C62340) Report Complete 
  • TOX-23 (NTIS No. PB93-150092)  (Peer Review 11/21/1991)
    Toxicity Studies ofo-,m-, andp- Nitrotoluenes (CAS Nos. 88-72-2, 99-08-1, 99-99-0) Administered in Dosed Feed to F344/N Rats and B6C3F1Mice
  • Rats: F344/N; Mice: B6C3F1
  • Dose: R&M: 0, 625, 1250, 2500, 5000, 10000 PPM/10 PER GROUP

Long-Term Carcinogenicity

• 2-YEAR (Dosed-Feed)  (C62340D) Report Complete 
  • TR-504 (NTIS No. PB2002-108715)  (Peer Review 05/03/2001)
    Toxicology and Carcinogenesis Studies ofo-Nitrotoluene (CAS No. 88-72-2) in F344/N Rats and B6C3F1 Mice (Feed Studies)
  • Rats: F344/N; Mice: B6C3F1
  • Carcinogenesis Results
  • Male Rats Clear Evidence 
  • Female Rats Clear Evidence 
  • Male Mice Clear Evidence 
  • Female Mice Clear Evidence 
  • Dose: MR: 0, 625, 1250, 2000, OR 5000 PPM FR: 0, 625, 1250, OR 2000 PPM M: 0, 1250, 2500, OR 5000 PPM; 60/SEX/DOSE

Special Studies

• Absorption Disposition Metabolism Elimination (Gavage)  (S0206) Completed 
  • M Rats: F344/N
  • Dose: 260 MG/KG

Genetic Toxicology

• In Vitro Cytogenetics (CA/SCE)  (573604) Completed 
  • Citation: Galloway, S., Armstrong, M., Reuben, C., Colman, S., Brown, B., Cannon, C., Bloom, A., Nakamura, F., Ahmed, M., Duk, S., Rimpo, J., Margolin, B., Resnick, M., Anderson, B., and Zeiger, E. Chromosome aberration and sister chromatid exchanges in Chinese hamster ovary cells: Evaluations of 108 chemicals. Environ. Molec. Mutagen. Vol. 10 (Suppl 10) (1987) 1-176
  • Chromosome Aberation Negative 
  • Sister Chromatid Exchange Positive 
• Micronucleus  (A25222) Completed 
  • Mice: B6C3F1
  • Male Inconclusive 
  • Female Negative 
• Micronucleus  (A29734) Completed 
  • Mice: B6C3F1
  • Male Negative 
• Micronucleus  (A80173) Completed 
  • Rats: F344
  • Male Negative 
• Salmonella  (192331) Completed 
  • Citation: Haworth, S., Lawlor, T., Mortelmans, K., Speck, W., and Zeiger, E. Salmonella mutagenicity test results for 250 chemicals Environ. Mutagen. Vol. 5 (Suppl 1) (1983) 3-142
  •  Negative 
• Salmonella  (692737) Completed 
  • Citation: Haworth, S., Lawlor, T., Mortelmans, K., Speck, W., and Zeiger, E. Salmonella mutagenicity test results for 250 chemicals Environ. Mutagen. Vol. 5 (Suppl 1) (1983) 3-142
  •  Negative