Japour AJ, Welles S, McIntosh K, Colson A, Chung P, Lockman S, Davenny K, Fowler MG, Hansen IC, Kalish L, Moye J, Rick K, Zorilla C, Pitt J; International Conference on AIDS.
Int Conf AIDS. 1996 Jul 7-12; 11: 370 (abstract no. Tu.C.2606).
Beth Israel Hospital, Boston, MA, USA. Fax: 617-667-5541. E-mail: tjapour@bih.harvard.edu.
Objective: To determine the prevalence of mutations conferring ZDVR among women treated with ZDV during pregnancy and among HIV-infected infants born to women treated with ZDV during pregnancy. Methods: Viral isolates at delivery from 48 women treated with ZDV during pregnancy and from the 9 HIV-infected infants born to these mothers were analyzed for the presence of HIV reverse transcriptase (RT) mutations associated with ZDVR. RNA was extracted and reverse transcribed; cDNA was amplified using nested PCR. The DNA was sequenced using dye-primer chemistry (ABI). The 6 mutations of interest were M41L, D67N, K70R, L210W, T215Y, K219Q. Results: The HIV RT was successfully amplified in 45/48 (94%) of the women and 9/9 (100%) of the infants. Thus far we have sequenced DNA from 14 women and 6 infants. Three of 14 (21%) women were found to carry a mutation at one or more of the six codons of interest: K70R alone (n=2) and M41L and T215/y(n=1). Viruses from an index mother infant pair in this cohort had previously been found to carry ZDVR mutations. Sequential isolates of the index mother and infant were analyzed: (table: see text) Conclusions: ZDVR mutations were prevalent among mothers in the WITS cohort who were treated with ZDV during pregnancy. The occurrence of maternal virus with a single mutation at codon 70 suggests that one should screen for this mutation in addition to the 41/125 codons. Sequential analysis of HIV isolates from one mother showed the evolution of the HIV quasi-species of T/S at codon 215 to Y during gestation.
Publication Types:
Keywords:
- Acquired Immunodeficiency Syndrome
- Anti-HIV Agents
- Base Sequence
- Codon
- Disease Transmission, Vertical
- Female
- HIV
- HIV Infections
- HIV Seropositivity
- Humans
- Infant
- Mutation
- Pregnancy
- Prevalence
- Zidovudine
- genetics
- reverse transcriptase, Human immunodeficiency virus 1
- transmission
Other ID:
UI: 102218688
From Meeting Abstracts