National Toxicology Program

CASRN: 67-66-3

Formula: C-H-CL3

Synonyms/Common Names:

• TRICHLOROMETHANE

Known Uses:

CHEMICAL INTERMEDIATE FOR FLUOROCARBON 22, MILDEWCIDE FOR TOBACCO SEEDLINGS, SOIL FUMIGANT & INSECTICIDE, DRYCLEANING AGENT, CHEMICAL INTERMEDIATE FOR DYES (HSDB 1990)

Short-Term Toxicity

• 14-Day (Gavage)  (C02686) Completed 
  • NTIS No. PB264018  (Peer Review 09/00/1976)
    Citation: Report on the Carcinogenesis Bioassay of Chloroform (CAS No. 67-66-3).
  • Rats: Osborne Mendel; Mice: B6C3F1
• 13-Week (Gavage)  (C02686) Completed 
  • NTIS No. PB264018  (Peer Review 09/00/1976)
    Citation: Report on the Carcinogenesis Bioassay of Chloroform (CAS No. 67-66-3).
  • Rats: Osborne Mendel; Mice: B6C3F1

Long-Term Carcinogenicity

• 2-Year (Gavage)  (C02686) Report Complete 
  • TR-000 (67-66-3) (NTIS No. PB264018)  (Peer Review 09/00/1976)
    Report on the Carcinogenesis Bioassay of Chloroform (CAS No. 67-66-3).
  • Rats: Osborne Mendel; Mice: B6C3F1
  • Carcinogenesis Results
  • Male Rats Positive 
  • Female Rats Negative 
  • Male Mice Positive 
  • Female Mice Positive 
  • Dose: MR:0,90,180 FR:0,125,250 (WK 1-22) 0,90,180 (WK23-SAC) FM:0,7,14 MM:0,4,8 MG/KG/50 PER GROUP

Special Studies

• Absorption Disposition Metabolism Elimination (Intraperitoneal Injection; Dosed-Water)  (S0059) Completed 
  • M Rats: F344/N; M Rats: F344/N

Genetic Toxicology

• Chromosome Aberrations  (142011) Completed 
  • Mice
  • Chromosome Aberation  Negative 
• In Vitro Cytogenetics (CA/SCE)  (564651) Completed 
  • Chromosome Aberation Inconclusive 
  • Sister Chromatid Exchange Negative 
• Mouse Lymphoma (NCI)  (907612) Completed 
  • Citation: Myhr, B.C., and Caspary, W.J. Evaluation of the L5178Y mouse lymphoma cell mutagenesis assay: Intralaboratory results for sixty-three coded chemicals tested at Litton Bionetics, Inc. Environ. Molec. Mutagen. Vol. 12 (Suppl 13) (1988) 103-194
  • Mouse Lymphoma-NCI  Positive 
• Mouse Lymphoma  (994137) Completed 
  • Citation: Mitchell, A.D., Rudd, C.J., and Caspary, W.J. Evaluation of the L5178Y mouse lymphoma cell mutagenesis assay: Intralaboratory results for 63 coded chemicals tested at SRI International. Environ. Molec. Mutagen. Vol. 12 (Suppl 13) (1988) 37-102
  •  Positive 
• Micronucleus  (142011) Completed 
  • Mice: B6C3F1
  • Male Positive 
• Salmonella  (749633) Completed 
  •  Negative 
• Sister Chromatid Exchanges  (142011) Completed 
  • Mice
  • Sister Chromatid  Negative 

Organ Systems Toxicity

• 14 DAYS Immunotoxicity (Gavage)  (IMM92050) Completed 
  • F Mice: B6C3F1
  • Dose: 0, 25, 50, 100, 150, 200, 300, 400, 500, 600, 700, OR 800 MG/KG
• Continuous Breeding (Gavage)  (RACB87045) Completed 
  • NTIS No. PB89-148639
    Citation: Chloroform Reproduction and Fertility Assessment in CD-1 Mice When Administered By Gavage
  • M/F Mice: Swiss
  • Dose: N/A; 0,6.6,15.9,41.2 MG/KG; N/A;0,41.2 MG/KG