Quantitative Mass Spectrometry

Mass Spectrometry Group

Quantitative Mass Spectrometry incorporates collaborative projects in which the Mass Spectrometry Group provides quantitative information, typically about, small molecules, by GC/MS, LC/MS and LC/MS/MS or a combination.

The Mass Spectrometry Group has extensive experience in focused metabonomics. Focused metabonomics refers to the analysis of specific targeted metabolic products, in contrast to studies that attempt to identify changes in all metabolites produced by a system, often without identifying specific components. The majority of these studies have focused on eicosanoid and prostaglandin metabolites from blood, tissue and urine.

For example, these types of project include the isoprostane analyses undertaken as part of the NIEHS-led study of biomarkers of oxidative stress. As part of an on-going collaboration with Darryl Zeldin (LRB), the MS Group has successfully quantitated hydroxyeicostetraenoic acids (HETEs) and epoxyeicosatrienoic acids (EETs) in a variety of sample types, including lung tissue and bronchoalveolar lavage fluid, kidney tissue and urine, heart tissue, gastrointestinal tissues, liver tissue and brain tissue.

In another collaboration, we are collaborating on a program projects involving the University of North Carolina and Roswell Institute (James Mohler) on Interference with the Androgen Receptor and its Ligands in Recurrent Prostate Cancer.