National Toxicology Program

CASRN: 16071-86-6

Formula: C31-H18-CU-N6-O9-S.2NA

Synonyms/Common Names:

• BROWN 95, C.I. DIRECT
• (5-((4'-((2,6-DIHYDROXY-3-((2-HYDROXY-5-SULFOPHENYL)-AZO)(1,1'-BIPHENYL)-4-YL)AZO)-2-HYDROXYBENZOATO(4-))-CUPRATE(2-)DIS0DIUM SALT (9CI)

Known Uses:

DYE FOR TEXTILES, LEATHER, PLASTICS, PAPER

Short-Term Toxicity

• 14-Day (Dosed-Feed)  (C54568) Completed 
  • NTIS No. PB280204  (Peer Review 03/06/1978)
    Citation: 13-Week Subchronic Toxicity Studies of Direct Blue 6, Direct Black 38, and Direct Brown 95 Dyes
  • Rats: F344/N; Mice: B6C3F1
  • Dose: R: 0,190,375,750,1500,3000, MM: 0,750,1500,3000,6000,12500, FM: 0,375,750,1500,3000,6000 PPM/10 PER GROUP
• 13-Week (Dosed-Feed)  (C54568) Report Complete 
  • NTIS No. PB280204  (Peer Review 03/06/1978)
    Citation: 13-Week Subchronic Toxicity Studies of Direct Blue 6, Direct Black 38, and Direct Brown 95 Dyes
  • Rats: F344/N; Mice: B6C3F1
  • Dose: R: 0,190,375,750,1500,3000, MM: 0,750,1500,3000,6000,12500, FM: 0,375,750,1500,3000,6000 PPM/10 PER GROUP

Genetic Toxicology

• In Vitro Cytogenetics (CA/SCE)  (754489) Completed 
  • Citation: Galloway, S., Armstrong, M., Reuben, C., Colman, S., Brown, B., Cannon, C., Bloom, A., Nakamura, F., Ahmed, M., Duk, S., Rimpo, J., Margolin, B., Resnick, M., Anderson, B., and Zeiger, E. Chromosome aberration and sister chromatid exchanges in Chinese hamster ovary cells: Evaluations of 108 chemicals. Environ. Molec. Mutagen. Vol. 10 (Suppl 10) (1987) 1-176
  • Chromosome Aberation Negative 
  • Sister Chromatid Exchange Inconclusive 
• Drosophila  (205697) Completed 
  • Citation: Yoon, J.S., Mason, J.M., Valencia, R., Woodruff, R.C., and Zimmering, S. Chemical mutagenesis testing in Drosophila: IV. Results of 45 coded compounds tested for the National Toxicology Program. Environ. Mutagen. Vol. 7 (1985) 349-367
  • Reciprocal Translocation/Sex-Linked Recessive Lethal Negative 
• Mouse Lymphoma  (162126) Completed 
  •  Positive 
• Salmonella  (306945) Completed 
  • Citation: Mortelmans, K., Haworth, S., Lawlor, T., Speck, W., Tainer, B., and Zeiger, E. Salmonella mutagenicity tests. II. Results from the testing of 270 chemicals Environ. Mutagen. Vol. 8 (Suppl 7) (1986) 1-119
  •  Weakly Positive 
• Salmonella  (870768) Completed 
  • Citation: Mortelmans, K., Haworth, S., Lawlor, T., Speck, W., Tainer, B., and Zeiger, E. Salmonella mutagenicity tests. II. Results from the testing of 270 chemicals Environ. Mutagen. Vol. 8 (Suppl 7) (1986) 1-119
  •  Negative 
• Salmonella Reduction  (599630) Completed 
  • Citation: Reid, T., Morton K., and King, C. Mutagenicity of azo dyes following metabolism by different reductive/oxidative systems. Environ. Mutagen. Vol. 6 (1984) 705-717
  •  Positive 

Organ Systems Toxicity

• GD 6-15 Conventional Teratology (Subcutaneous Injection)  (T0139) Completed 
  • F Mice: Swiss
  • Dose: 0, 20, 40, 60, 80, 100 MG/KG/DAY